We narrowed to 25,057 results for: Nes

1. CAG sHRPa-NRX

   Plasmid

   #73143

   Purpose

   Large fragment of split HRP fused to the extracellular terminus of neurexin3B. CAG promoter.

   Depositor

   Alice Ting

   Article

   Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

   Insert

   sHRPa-NRX
   

   Use

   Tags

   2x HA tag and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-PGC1

   Plasmid

   #4

   Depositor

   Bruce Spiegelman

   Article

   Puigserver et al Cell 1998 Mar 20;92(6):829-39.

   Insert

   PGC-1 alpha (Ppargc1a Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 4, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOlyA(E69A)

   Plasmid

   #132392

   Purpose

   Mutant version of OlyA that senses sphingomyelin without or with cholesterol

   Depositor

   Arun Radhakrishnan

   Article

   Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

   Insert

   Ostreolysin A point mutant (E69A)
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Mutations: C62S, E69A, C94S, S151C

   Promoter

   Available Since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Desmoglein2-mCherry

   Plasmid

   #36991

   Depositor

   Kathleen Green

   Article

   Nekrasova et al J Cell Biol. 2011 Dec 26;195(7):1185-203

   Insert

   Dsg2 (DSG2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. 5UAS zfSynaptophysin:GFP-5UAS DsRedExpress

   Plasmid

   #74317

   Purpose

   A fusion of zebrafish synaptophysin to GFP and DsRedExpress under the control of separate UAS cassettes; for dual-labeling of retinal ganglion cell (RGC) axon arbors in zebrafish

   Depositor

   Martin Meyer, Stephen Smith

   Article

   Meyer et al J Neurosci. 2006 Mar 29;26(13):3604-14.

   Inserts

   synaptophysin
   DsRedExpress
   

   Use

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   5UAS

   Available Since

   May 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRCC-N

   Plasmid

   #81192

   Purpose

   Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance

   Depositor

   Eckhard Boles

   Article

   Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

   Inserts

   Cas9
   empty gRNA cassette
   

   Use

   CRISPR

   Tags

   NTS

   Expression

   Yeast

   Mutation

   Please see depositor comments below. and WT - cod…

   Promoter

   ROX3 and SNP52p

   Available Since

   Sept. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCT310

   Plasmid

   #111140

   Purpose

   Bacterial expression of 6His-dCas9-3XFlag

   Depositor

   Robert Tjian

   Article

   Tsui et al Proc Natl Acad Sci U S A. 2018 Mar 20;11

   Insert

   dCas9
   

   Use

   Tags

   3X Flag and 6X His

   Expression

   Bacterial

   Mutation

   D10A - H840A

   Promoter

   T7

   Available Since

   July 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE EF1α dCas9-VP64

   Plasmid

   #50918

   Purpose

   Constitutive dCas9-VP64 lentiviral expression vector

   Depositor

   Scot Wolfe, Rene Maehr

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and VP64

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   EF1alpha

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSCV JMJD3 mutant

   Plasmid

   #21214

   Depositor

   Paul Khavari

   Article

   Sen et al Genes Dev. 2008 Jul 15. 22(14):1865-70.

   Insert

   JMJD3 (KDM6B Human)
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   H1390A

   Promoter

   Available Since

   June 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. mEGFP-RhoA-C1

    Plasmid

    #29674

    Depositor

    Ryohei Yasuda

    Article

    Murakoshi et al Nature. 2011 Apr 7;472(7341):100-4. doi:

    Insert

    RhoA (RHOA Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRU295

    Plasmid

    #167694

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::Cas9i
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFUGW-hGtACR2-EYFP

    Plasmid

    #67877

    Purpose

    Mammalian expression construct of human codon-adapted anion channelrhodopsin 2 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backbone

    Depositor

    John Spudich

    Article

    Govorunova et al Science. 2015 Jun 25. pii: aaa7484.

    Insert

    hGtACR2
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRTC2-puro

    Plasmid

    #190567

    Purpose

    Expresses the full-length human L1 (L1.3) retrotransposon and an engineered neomycin retrotransposition reporter to monitor retrotransposition efficiency in cultured mammalian cells.

    Depositor

    Anthony Furano

    Article

    Cook et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    L1 (L1.3) with reporter cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLX317 GFP

    Plasmid

    #193681

    Purpose

    Constitutive lentiviral expression of GFP

    Depositor

    William Hahn

    Article

    Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBI-p53 R175H/EGFP

    Plasmid

    #16544

    Depositor

    Bert Vogelstein

    Article

    Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R175H

    Promoter

    Available Since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSpyB-GFP

    Plasmid

    #220190

    Purpose

    sgRNA expression vector - pBG35 promoter with GFP in sgRNA site

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    pBG35 promoter with GFP in sgRNA site
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    pBG35

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMU-PMr

    Plasmid

    #61180

    Purpose

    Fluorescent reporter for plasma membrane AtPIP2a-mCherry expressed under AtUBQ10 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    AtPIP2a
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10

    Available Since

    April 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. SpCas9

    Plasmid

    #188489

    Purpose

    Expresses FLAG tagged SpCas9

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. 2C-3XtbGFP-PEST

    Plasmid

    #69072

    Purpose

    Expresses 3XturboGFP-NLS-PEST under MERVL_LTR

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Ishiuchi et al Nat Struct Mol Biol. 2015 Aug 3. doi: 10

    Insert

    turboGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. AB65_pCAG.Cas9-D10A.rBGpA

    Plasmid

    #199256

    Purpose

    Expression construct encoding the SpCas9-D10A nickase

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Insert

    SpCas9.D10A nickase
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CAG promoter

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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