165,803 results

1. pNC-Cam1304-RNAi

   Plasmid

   #193399

   Purpose

   Nimble cloning-compatible vector for plant RNAi

   Depositor

   Pu Yan

   Article

   Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. NP-11-64-1

   Plasmid

   #234359

   Purpose

   Expressing rubisco and prk in Δrpi

   Depositor

   David Savage

   Article

   Prywes et al Nature. 2025 Jan 22. doi: 10.1038/s41586

   Insert

   R.rubrum rubisco
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptac

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-EF1a-hChR2(H134R)-EYFP-WPRE

   Plasmid

   #20942

   Purpose

   3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by EF1a promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   H134R mutation in ChR2

   Promoter

   EF1alpha

   Available Since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. LentiV_Neo_SIK3

   Plasmid

   #108103

   Purpose

   Lentiviral expression plasmid of human SIK3 cDNA with neomycin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

   Insert

   SIK3 (SIK3 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS promoter

   Available Since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. Prab-3::NLS::GFP

   Plasmid

   #68120

   Purpose

   Nuclear Localized GFP for pan-neuronal expression in C. Elegans

   Depositor

   Andrew Leifer

   Article

   Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

   Insert

   GFP
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   rab-3

   Available Since

   Aug. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a Gx-mNG-NL

   Plasmid

   #169106

   Purpose

   Expresses protein G fused to mNeonGreen and NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

   Depositor

   Maarten Merkx

   Article

   Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

   Insert

   protein G - mNeonGreen - NanoLuc
   

   Use

   Tags

   his-tag and strep-tag

   Expression

   Bacterial

   Mutation

   Ala 24 stop

   Promoter

   T7

   Available Since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. FLAG-Skp2

   Plasmid

   #89142

   Purpose

   mammalian expression of human Skp2 with N-terminal FLAG tag

   Depositor

   Elizabeth Wilson

   Article

   Su et al Mol Cell Endocrinol. 2017 Jan 5;439:1-9.

   Insert

   Skp2 (SKP2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-(empty)-TAG

   Plasmid

   #138209

   Purpose

   To facilitate restriction cloning of an RNA of interest with a TGT recognition element in the 3' region

   Depositor

   Neal Devaraj

   Article

   Busby et al Methods Enzymol. 2020;641:373-399. doi:

   Type

   Empty backbone

   Use

   T7 transcription

   Tags

   TAG sequence

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Promoter, T7 Promoter

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET263-pUC57 24xMS2V7

   Plasmid

   #140705

   Purpose

   MS2V7 cloning

   Depositor

   Robert Singer, Evelina Tutucci

   Article

   Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

   Insert

   24xMS2V7
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   24 MS2 loops V7, high-affinity C-variant, inter-s…

   Promoter

   None

   Available Since

   April 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-6xHis-MBP-TEV site-TadA8r

    Plasmid

    #208318

    Purpose

    Overxpression MBP-TadA8r

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA8r
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mutations in TadA are described in the paper

    Promoter

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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