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pFETCh_CIC_iso1

Plasmid

#135728

Purpose

Donor vector for 3' FLAG tag of human CIC_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

CIC_iso1 Homology arms (CIC Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmPAN3-W707AG711E-dsRNAres_Y

Plasmid

#148087

Purpose

Insect Expression of DmPAN3-W707AG711E-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN3-W707AG711E-dsRNAres (CG11486 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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MCV-Rep-

Plasmid

#32058

Depositor

Patrick Moore

Article

Feng et al PLoS One. 2011;6(7):e22468. Epub 2011 Ju

Insert

MCV-Rep- genome

Mutation

Single cytosine to adenine (C/A) mutation at 2,07…

Promoter

none from vector. Insert contains native promoter…

Available Since

Oct. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pMIR-Dsred-IRES-His Keap

Plasmid

#62457

Purpose

expresses his tagged human Keap1 protein

Depositor

Yimon Aye

Article

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Keap1 (KEAP1 Human)

Tags

HisTag

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pKH163_pSmart-mNEON-YIPF4

Plasmid

#214982

Purpose

endogenous mneon tag for YIPF4

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

mNEON with YIPF4 homology (YIPF4 Human)

Use

CRISPR

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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HS-DN-Ect2

Plasmid

#105956

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Dzafic et al Cell Rep. 2015 Oct 6;13(1):168-182. doi:

Insert

Ect2 (ECT2 Human)

Tags

EGFP

Expression

Bacterial

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-Akt2(T309A)

Plasmid

#86625

Purpose

Expresses eGFP-tagged human Akt2(T309A) phosphomutant

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt2(T309A) (AKT2 Human)

Expression

Mammalian

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pETSUMO-TtAgocatdead

Plasmid

#210322

Purpose

Bacterial expression of the His- and SUMO-tagged catalytically inactive Argonaute protein of Thermus thermophilus

Depositor

Phillip Zamore

Article

Jolly et al Cell. 2020 Sep 17;182(6):1545-1559.e18.

Insert

TtAgo (TT_P0026 Thermus thermophilus)

Tags

6x His tag and SUMO tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-NIrD-Mefv iso1

Plasmid

#92108

Purpose

inducible expression in eukaryotic cells

Depositor

Feng Shao

Article

Gao et al Proc Natl Acad Sci U S A. 2016 Aug 16;11

Insert

Mefv isoform1 (Mefv Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pPSP6

Plasmid

#48150

Purpose

Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage SP6

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pIRES.Puro.mBeta

Plasmid

#24175

Depositor

Robert Sobol

Article

Jelezcova et al Mutat Res. 2010 Apr 1;686(1-2):57-67. do

Insert

pIRES.Puro.mBeta (Polb Mouse)

Expression

Mammalian

Mutation

x

Available Since

April 9, 2010

Availability

Academic Institutions and Nonprofits only
KHBD00233

Plasmid

#34377

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG7803 (z Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00322

Plasmid

#34447

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG8591 (CTCF Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pET28a(+) 6xHis-TEV-IFIT5

Plasmid

#53560

Purpose

bacterial expression of IFIT5

Depositor

Kathleen Collins

Article

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT5 (IFIT5 Human)

Tags

6xHIS and TEV

Expression

Bacterial

Promoter

T7

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only
pRS415-Cas9-VPR

Plasmid

#163971

Purpose

Trifunctional Cas9-VPR fusion protein plasmid for simultaneous transcriptional activation, transcriptional repression, and genome editing in yeast

Depositor

Jiazhang Lian

Article

Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

Insert

Cas9-VPR

Use

Synthetic Biology

Expression

Yeast

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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AQTFAATHNPWASQA_epitope

Plasmid

#165019

Purpose

Expression of CMV UL86 (1222-1236) fused to CD74

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

CMV UL86 (1222-1236)

Use

Lentiviral

Tags

CD74 at N-term; cathepsin S cleavage site (GRWHTV…

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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KAP3-CT

Plasmid

#51396

Purpose

expression of EGFP-tagged COOH-terminal AA 661–793 KAP3B in mammalian cells

Depositor

Trina Schroer

Article

Brown et al Traffic. 2005 Dec;6(12):1114-24.

Insert

Kinesin-associated protein 3 (KIFAP3 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2014

Availability

Academic Institutions and Nonprofits only
pET28a-HsSENP5

Plasmid

#71468

Purpose

Encodes human full length SENP5 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

Depositor

Jorge Eduardo Azevedo

Article

Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

Insert

SUMO1/sentrin specific peptidase 5 (SENP5 Human)

Tags

Hexahistidine

Expression

Bacterial

Mutation

Gene optimised for E. coli expression (Genscript)

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pENTR1A-3xFlag-M694V-MEFV

Plasmid

#167118

Purpose

Gateway entry plasmid with 3xFlag-pyrin (M694V/FMF variant)

Depositor

Thomas Henry

Article

Chirita et al Methods Mol Biol. 2022;2523:179-195. doi

Insert

MEFV (MEFV Human)

Use

Gateway entry vector

Tags

3xFlag

Mutation

M694V Methionine 694 to valine (FMF mutation)

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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KHBD00311

Plasmid

#34436

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG9786 (hb Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only