We narrowed to 11,615 results for: Vars;

1. pAAV.hSyn.FLEX.iGluSnFR3.v857.IgK-NGR

   Plasmid

   #196226

   Purpose

   Fluorescent reporter for glutamate, third generation, variant 857 in NGR backbone. iGluSnFR3.v857.NGR

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Insert

   pAAV hSyn FLEX iGluSnFR3 v857.NGR
   

   Use

   AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

   Tags

   IgK-chain, Myc epi tag, and NGR TM Domain

   Expression

   Mammalian

   Available Since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-Syn-FLPX-rc [ChrimsonR-tdTomato]

   Plasmid

   #128589

   Purpose

   AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Has Service

   AAV8

   Insert

   ChrimsonR-tdTomato
   

   Use

   AAV

   Tags

   tdTomato (codon diversified version)

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   Syn

   Available Since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. p38 WT O/E (MAPK14)-pcw107-V5

   Plasmid

   #64624

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   MAPK14 (transcript variant 2) (MAPK14 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Promoter

   PGK

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pLenti PGK mKate2-OGT D554N (Puro)

   Plasmid

   #154292

   Purpose

   Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, D554N variant, no Ser/Thr glycosylation)

   Depositor

   Suzanne Walker

   Article

   Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Lentiviral

   Tags

   2x FLAG and mKate2

   Expression

   Mammalian

   Mutation

   D554N mutation (Ser/Thr glycosylation-incompetent)

   Promoter

   PGK

   Available Since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWZL Neo Myr Flag RPS6KA5

   Plasmid

   #20622

   Depositor

   Jean Zhao, William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   RPS6KA5 (RPS6KA5 Human)
   

   Use

   Retroviral

   Tags

   Flag and Myr

   Expression

   Mammalian

   Available Since

   Oct. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

6. LLP340 pEF1a-NLS-scFvGCN4-DNMT3a (mutant)

   Plasmid

   #100944

   Purpose

   Single chain variable fragment antibody GCN4 fused to DNMT3a catalytic mutant, binds SunTag, with 3xTy1 tag

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   scFvGCN4, sfGFP, GB1, DNMT3a catalytic mutant (DNMT3A Synthetic, Human)
   

   Tags

   3xTy1

   Expression

   Mammalian

   Mutation

   F636A, E660A, E725A, R295A

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. R620-M13-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 B.1.1.7

   Plasmid

   #170197

   Purpose

   Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.1.7 variant)

   Depositor

   Dominic Esposito

   Article

   Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

   Insert

   SARS-CoV-2 S (spike) B.1.1.7 (S SARS-CoV-2)
   

   Tags

   His6

   Expression

   Mammalian

   Mutation

   Δ(69-70), Δ144, N501Y, A570D, D614G, P681H, T716I…

   Promoter

   CMV51

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-DIO {mCAR}off-{ChETA-mRuby2}on-W3SL

   Plasmid

   #111390

   Purpose

   AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection), Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)

   Depositor

   Adam Kepecs

   Article

   Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

   Inserts

   mCAR (Cxadr Mouse)
   ChETA-mRuby2
   

   Use

   AAV

   Tags

   Myc and mRuby2

   Expression

   Mammalian

   Promoter

   hSynapsin

   Available Since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-BACH1t_RFP

   Plasmid

   #199214

   Purpose

   BACH1 C-terminus truncated isoform - BACH1t expression plasmid with a RFP reporter on the backbone.

   Depositor

   Gabor Balazsi

   Article

   Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

   Inserts

   BACH1t (BACH1 Human)
   RFP
   

   Expression

   Mammalian

   Promoter

   CMV and PGK

   Available Since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti PGK mKate2-OGT 5N5A (Puro)

    Plasmid

    #154293

    Purpose

    Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, 5N5A variant, no HCF-1 cleavage)

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Lentiviral

    Tags

    2x FLAG and mKate2

    Expression

    Mammalian

    Mutation

    N322A, N356A, N390A, N424A, N458A mutations; HCF-…

    Promoter

    PGK

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV.CAG-FLEX.iAChSnFR-Venus-NULL

    Plasmid

    #137960

    Purpose

    Expresses FLEXed iAChSnFR (non-binding yellow version) under CAG promoter

    Depositor

    Loren Looger

    Article

    Borden et al bioRxiv 2020

    Insert

    iAChSnFR (non-binding yellow variant)
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Mutation

    Y140A binding site mutation

    Promoter

    CAG

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hSyn-DIO {hCAR}off-{ChETA-mRuby2}on-W3SL

    Plasmid

    #111391

    Purpose

    AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)

    Depositor

    Adam Kepecs

    Article

    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

    Inserts

    hCAR (CXADR Human)
    ChETA-mRuby2
    

    Use

    AAV

    Tags

    Myc and mRuby2

    Expression

    Mammalian

    Promoter

    hSynapsin

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCJ209

    Plasmid

    #162682

    Purpose

    Lidless variant of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) replacing Gly251:Thr472 of MHETase with the 7-residue loop (Trp185:Phe191, PETase numbering) of PETase , codon optimized for expression in E. coli K12, with C-terminal His tag.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with the lid removed
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Replacement of G251-T472 with W185-F191 from PETa…

    Promoter

    T7/lac

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. R620-M14-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 B.1.351

    Plasmid

    #170198

    Purpose

    Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.351 variant)

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    SARS-CoV-2 S (spike) B.1.351 (S SARS-CoV-2)
    

    Tags

    His6

    Expression

    Mammalian

    Mutation

    L18F, D80A, D215G, Δ(242-244), R246I, K417N, E484…

    Promoter

    CMV51

    Available Since

    May 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-sACE2v2(732)-IgG1

    Plasmid

    #154105

    Purpose

    Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2 (IgG1-Fc fusion)

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Tags

    Fc region of human IgG1

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease and neck domains …

    Promoter

    CMV

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. ERalpha (Y537S mutant)-pcw107-V5

    Plasmid

    #64634

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    ESR1 (transcript variant 1) (ESR1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Mutation

    Y537S

    Promoter

    PGK

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3-sACE2v2(732)-8h

    Plasmid

    #154102

    Purpose

    Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2 (8his tag)

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Tags

    Short GSG linker and 8his tag

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available Since

    June 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGS-Omicron.BA.5-RBD

    Plasmid

    #213478

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.BA.5 variant

    Depositor

    Priyamvada Acharya

    Article

    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

    Insert

    pCAGGS/Omicron.BA.5 RBD (S )
    

    Tags

    6 His Tag

    Expression

    Mammalian

    Mutation

    G339D,R346T,S371F,S373P,S375F,T376A,D405N,R408S,K…

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS/EG5-RBD

    Plasmid

    #213410

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.EG5 variant

    Depositor

    Priyamvada Acharya

    Article

    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

    Insert

    pCAGGS/EG5-RBD (S )
    

    Tags

    6 His Tag

    Expression

    Mammalian

    Mutation

    G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn-DIO {hCAR}off-{ChETA-EYFP}on-W3SL

    Plasmid

    #111392

    Purpose

    AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON ChETA-EYFP (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)

    Depositor

    Adam Kepecs

    Article

    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

    Inserts

    hCAR (CXADR Human)
    ChETA-EYFP
    

    Use

    AAV

    Tags

    EYFP and Myc

    Expression

    Mammalian

    Promoter

    hSynapsin

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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