We narrowed to 23,077 results for: ESC
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Plasmid#58099PurposeLocalization: Centrosomes, Excitation: 554, Emission: 581DepositorAvailable SinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only
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WT C-His Ana GvpC in pET28a
Plasmid#85732PurposeExpresses His-tagged GvpC from Anabaena flos-aquae in E.coliDepositorInsertGas vesicle protein C (his-tagged)
TagsHis-tagExpressionBacterialMutationAdded an extra glycine after the methionine at th…PromoterT7Available SinceMarch 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAT003
Plasmid#171628Purpose2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and an EF1-a promoter expressing mNeonGreenDepositorInsertsspyCas9 sgRNA-TRAC
mNeon
UseCRISPR and LentiviralExpressionMammalianPromoterCAG and EF1-aAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-BBS2
Plasmid#218712PurposeExpresses N-terminally EGFP-tagged BBS2 in mammalian cellsDepositorAvailable SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBMN ltDHFR(DD)-YFP
Plasmid#47076PurposeecDHFR based destable domain function at low temperature for regulating protein stability in Caenorhabditis elegansDepositorInsertltDHFR(DD)
UseRetroviralTagsHA and YFPMutationamino acids mutations: R12H, N23S, G67S, V78A, E1…PromoterLTRAvailable SinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHL 1158
Plasmid#53007PurposepLtetO-1:RhyB, pCon:DsrA, pCon:ompC::mCherry, pLlacO-1:sodB::gfpDepositorInsertsRhyB sRNA
DsrA sRNA
ompC
sodB
UseSynthetic BiologyTagsgfp and mCherryExpressionBacterialMutationcontains -56 to +141 relative to start codon and …PromoterpCon, pLlacO-1, and pLtetO-1Available SinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
Cas9-sgRNA-A+B
Plasmid#149370PurposeExpresses SpCas9 and two sgRNA targeting the lenti-CDDR reporter at two distal sitesDepositorInsertsCas9
sgRNA-A
sgRNA-B
Puromycin resistance
UseCRISPRTags3xFLAG, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianPromoterCBh, PGK, and U6Available SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
GST-CHMP4A (1-204) (pGEX2T(TEV))
Plasmid#80632PurposeResidues 1-204 of CHMP4A; Bacterial Expression vector; TEV-cleavable GST tag; Sundquist Lab internal ID: WISP06-198DepositorAvailable SinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDH_Str-KDEL_ST-SBP-EGFP
Plasmid#65267Purposesynchronize trafficking of ST from the ER (RUSH system)DepositorInsertStr-KDEL and truncated sialyltransferase (ST6GAL1 Human)
UseLentiviralTagsEGFP and Streptavidin Binding Protein (SBP)Mutationcontains only amino acids 1 to 43 of STPromoterCMVAvailable SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
011LP-pAAV-TNT4-EmGFP-pA
Plasmid#120280PurposeAAV vector for cardiomyocyte specific expression of Emerald Green Fluorescent Protein using cardiac troponin T promoterDepositorInsertEmGFP
UseAAVTags3XFlag and HAPromoterTNT Cardiac Troponin T promoterAvailable SinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyso-ExRai-AktAR2
Plasmid#184052PurposeLysosome-targeted ExRai-AktAR2.DepositorInsertLyso-ExRai-AktAR2
TagsLysosome-associated membrane protein 1 (LAMP1).ExpressionMammalianPromoterCMVAvailable SinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_NSnoFbox-vhhGFP4
Plasmid#35580DepositorAvailable SinceApril 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFlp-Gm
Plasmid#222335PurposeExpresses Flp and gentamicin to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-gent-pBBR1-ELT3DepositorAvailable SinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO_TOMM20_HaloTag9
Plasmid#169335PurposeExpression of HaloTag9 at the mitochondrial surface of mammalian cellsDepositorInsertTOMM20-HaloTag9 (TOMM20 Human)
TagsHaloTag9ExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RAvailable SinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO GABARAP
Plasmid#218427PurposeExpression of tagless GABARAPDepositorAvailable SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL1-SpCas9
Plasmid#117051PurposeIn vitro expression of S. pyogenes Cas9 from the T7 promoterDepositorInsertS. pyogenes Cas9
ExpressionBacterialPromoterT7Available SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP - FLAG-APEX2- hs Lamin B1
Plasmid#139442PurposeAPEX2-Lamin B1 expression plasmidDepositorInsertLMNB1 (LMNB1 Human)
Tags3xGGGGS linker (between APEX2 and Lamin B1), APEX…ExpressionMammalianPromoterCMVAvailable SinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-myc
Plasmid#109422PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to a myc epitope. VHH-myc also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, myc, HA, BAP and His6 epitope
TagsBAP, HA, His6, T7, and mycExpressionBacterialPromoterT7Available SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
mTagRFP-T-LaminB1-10
Plasmid#58020PurposeLocalization: Nuclear Envelope, Excitation: 555, Emission: 584DepositorAvailable SinceNov. 4, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLPC H-Ras V12
Plasmid#18741DepositorAvailable SinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only