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pMSCVpuro-Gcn5

Plasmid

#63706

Purpose

retroviral expression of mouse Gcn5 in mammalian cells

Depositor

Kai Ge

Article

Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

Insert

Gcn5 (Kat2a Mouse)

Use

Retroviral

Tags

None

Expression

Mammalian

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only
pX458-DsRed2

Plasmid

#112219

Purpose

Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and DsRed2

Depositor

Yinming Liang

Article

Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

Insert

DsRed2

Use

CRISPR

Expression

Mammalian

Mutation

DsRed2 contains a silent nucleotide substitution …

Available Since

July 11, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCardinal

Plasmid

#51311

Purpose

Expresses far-red fluorescent protein mCardinal in mammalian cells

Depositor

Michael Lin

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2014

Availability

Academic Institutions and Nonprofits only

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pSLQ1658-dCas9-EGFP

Plasmid

#51023

Purpose

Template for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging (the recipient vector can be TetON 3G promoter system)

Depositor

Bo Huang, Stanley Qi

Article

Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

Insert

dCas9 fuse to EGFP

Use

CRISPR and Retroviral

Tags

EGFP

Expression

Mammalian

Promoter

MSCV LTR promoter

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pTag-BFP-C-h-Rab11a

Plasmid

#79805

Purpose

Express TagBFP labeled human Rab11a (GTPase located on recycling endosomal membranes).

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

Rab11a (RAB11A Human)

Tags

TagBFP

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pCCLc-MND-A0201-SABR-Backbone

Plasmid

#119050

Purpose

3rd gen transfer vector. HLA-A0201 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains a stuffer fragment flanked by BsmBI sites at the epitope cloning site.

Depositor

David Baltimore

Article

Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

MND

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pTag-BFP-C-h-Rab5a-c-Myc

Plasmid

#79801

Purpose

Express TagBFP labeled human Rab5a (GTPase located on early endosomal membranes).

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

Rab5a (RAB5A Human)

Tags

TagBFP and myc

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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scFv-sfGFP-DNMT3A1

Plasmid

#102278

Purpose

The plasmid encodes a single-chain antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a DNA methyltransferase DNMT3A1

Depositor

Margaret Goodell

Article

Huang et al Genome Biol. 2017 Sep 18;18(1):176. doi:

Insert

DNMT3A1 (DNMT3A Human)

Use

Lentiviral

Tags

scFV-sfGFP

Expression

Mammalian

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-EZH2

Plasmid

#28060

Depositor

Mien-Chie Hung

Article

Wei et al Nat Cell Biol. 2011 Jan . 13(1):87-94.

Insert

EZH2 (EZH2 Human)

Tags

GST

Expression

Bacterial

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only

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pBS31-cCARLIN

Plasmid

#160928

Purpose

Contains 10 constitutively expressed guide RNAs and CAG-GFP-CARLIN array sequence for DNA barcoding (when used in combination with Cas9)

Depositor

Fernando Camargo

Article

Bowling et al Cell. 2020 Jun 11;181(6):1410-1422.e27.

Insert

CARLIN gRNAs and CAG-GFP-CARLIN array

Use

Mouse Targeting

Expression

Mammalian

Promoter

U6 promoter for gRNAs; CAG promoter for GFP

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1hSIRT5_HA

Plasmid

#24483

Depositor

Eric Verdin

Article

Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

Insert

Sirtuin 5 (SIRT5 Human)

Tags

HA

Expression

Mammalian

Available Since

April 23, 2010

Availability

Academic Institutions and Nonprofits only

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GFP-JIP4

Plasmid

#164620

Purpose

Expresses JIP4 in mammalian cells with a GFP tag

Depositor

Mark Cookson

Article

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

JIP4 (SPAG9 Human)

Tags

cycle 3 GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG_mRuby2_smFP FLAG

Plasmid

#59760

Purpose

encodes multiple copies of an epitope tag FLAG ( not detected by anti GFP ab)

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

mRuby2_smFP_FLAG

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only

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Hygro-Cas9 donor

Plasmid

#86883

Purpose

Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus with hygromycin selection

Depositor

Danwei Huangfu

Article

Shi et al Cell Stem Cell. 2017 Feb 8. pii: S1934-5

Insert

hSpCas9

Use

CRISPR

Tags

3xFlag, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Available Since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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PE-P3-maize

Plasmid

#185611

Purpose

For plant prime editing in maize plants

Depositor

Jinxiao Yang

Article

Xu et al Nat Plants. 2022 Jan;8(1):45-52. doi: 10

Insert

3XFLAG-4XNLS-M-MLV-nCas9(H840A)-4XNLS

Use

CRISPR

Promoter

ZmUbi

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pH-ABE8e-spG

Plasmid

#200775

Purpose

For plant adenine base editing in rice plants or monocotyledons protoplasts

Depositor

Caixia Gao

Article

Xue et al Nat Biotechnol. 2023 Mar 9. doi: 10.1038

Insert

nCas9-spG(D10A)-TadA8e

Use

CRISPR

Expression

Plant

Mutation

D10A, D1135L, S1136W, G1218K, E1219Q, R1335Q, T13…

Promoter

maize Ubiquitin-1, OsU3

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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GABPA_pcDNA6.2/EmGFP-Bsd

Plasmid

#176970

Purpose

Mammalian expression vector encoding GABPA and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

GABPA (GABPA Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1hSIRT4_HA

Plasmid

#24482

Depositor

Eric Verdin

Article

Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

Insert

Sirtuin 4 (SIRT4 Human)

Tags

HA

Expression

Mammalian

Available Since

April 23, 2010

Availability

Academic Institutions and Nonprofits only

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pEGFP Kindlin2

Plasmid

#105305

Purpose

To study effect of Kindlin 2 on Cell/ECM adhesion

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-HaloSFPQ

Plasmid

#166948

Purpose

Lentiviral plasmid expressing Halo-tagged SFPQ protein from the EF1a promoter

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

SFPQ (SFPQ Human)

Use

Lentiviral

Tags

Halo

Promoter

eF1a

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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