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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 11161 - 11180 of 79426 results

  1. pSBBi-GB CaXMT1

    Plasmid
    #139168
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains Coffea arabica xanthosine methyltransferase 1 (CaXMT1); the other side contains GFP and blasticidin resistance genes.
    Depositor
    Neal Devaraj
    Insert
    7-methylxanthosine synthase 1 [Coffea arabica]
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1α
    Availability
    Academic Institutions and Nonprofits only
    139168-plasmid-map-sequence-id-309358

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  3. pGFP FAK Y397F

    Plasmid
    #50516
    Purpose
    This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.
    Depositor
    Kenneth Yamada
    Insert
    protein tyrosine kinase, mutated
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Y397F
    Promoter
    CMV
    Available Since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  4. pCAG-IRES2-p75NTR-GFP

    Plasmid
    #182947
    Purpose
    To express the rat p75NTR neurotrophin receptor. The gene contains an HA tag at the N-Terminal
    Depositor
    Pantelis Tsoulfas
    Insert
    p75NTR-mGFP
    Tags
    HA tag and mGFP
    Expression
    Mammalian
    Mutation
    The leader sequence is from preprotrypin
    Promoter
    CAG
    Available Since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    182947-plasmid-map-sequence-id-360205

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  5. pAWO-SUMO-3C_14M_zTDP2

    Plasmid
    #200512
    Purpose
    Expression of zebrafish TDP2 recombinant protein
    Depositor
    Antony Oliver
    Insert
    TDP2 (tdp2b Zebrafish)
    Tags
    HIS-SUMO
    Expression
    Bacterial
    Mutation
    L136N, A139S, C239T, K240R, K309Q, K314L, T315G, …
    Available Since
    Aug. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    200512-plasmid-map-sequence-id-396440

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  6. pDual_dsCas9_Blast_hs_NC_chr1

    Plasmid
    #214690
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr1
    Use
    CRISPR and Lentiviral
    Available Since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214690-plasmid-map-sequence-id-428775

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  7. pCAG-eCas9-GFP-U6-gRNA

    Plasmid
    #79145
    Purpose
    All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in human pluripotent stem cells
    Depositor
    Jizhong Zou
    Insert
    eSpCas9(1.1)
    Use
    CRISPR
    Tags
    2A-GFP
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    June 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    79145-plasmid-map-sequence-id-182555

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  9. LentiCRISPRv2-mCherry

    Plasmid
    #99154
    Purpose
    Lentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.
    Depositor
    Agata Smogorzewska
    Inserts
    Cas9
    mCherry
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    EFS-NS
    Available Since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    99154-plasmid-map-sequence-id-352449

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  14. pcDNA3.1-Flag

    Plasmid
    #208051
    Purpose
    Empty vector for mammalian expression, insert a Flag tag (with ATG) at XhoI site of pcDNA3.1 (-), xhoI site at 5' of flag tag destroyed, xhoI site at 3' of flag tag retained. XhoI in-frame with Flag
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208051-plasmid-map-sequence-id-418310

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  19. pSMPUW_6.17_Ex_Ef1a-v5-EGFP-SV40-Zeo

    Plasmid
    #128453
    Purpose
    lentiviral expression vector with Ef1a promoter, N-terminal V5 tag, EGFP gene, SV40 promoter, and ZeoResistance gene
    Depositor
    Lindy Barrett
    Insert
    Ef1a promoter, N-terminal V5 tag, EGFP gene, SV40 promoter, and Zeocin Resistance gene
    Use
    Lentiviral
    Available Since
    Oct. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    128453-plasmid-map-sequence-id-261484

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Showing: 11161 - 11180 of 79426 results