We narrowed to 4,845 results for: AAT

1. pSLIK_hygro_3xflag_NRF2(RR)

   Plasmid

   #136535

   Purpose

   Lentiviral expression vector for an inducible 3xflag-tagged NRF2(a1584c_a1586t_a1589g)

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   NRF2(1584A>C,1586A>T,1589A>G) (NFE2L2 Human)
   

   Use

   Lentiviral; Destinatioin vector for gateway cloni…

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   This plasmid is developed by mutating 3 base pair…

   Promoter

   Available since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-KrasG12D/sgKras/Cre

   Plasmid

   #99851

   Purpose

   Expresses Cre-recombinase, a barcoded HDR template to introduce a G12D mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

   Depositor

   Monte Winslow

   Article

   Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

   Insert

   Kras (Kras Mouse)
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

   Promoter

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. t1-405(del37/GAG)

   Plasmid

   #166129

   Purpose

   Expression of human talin-1 head (aa1-405) in E. coli. Contains 37 amino acid deletion in F1-loop, replaced with Gly-Ala-Gly. N-terminal His6, Xpress-epitopeDLYDDDDK) and enterokinase cleavage site.

   Depositor

   Vesa Hytönen

   Article

   Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13

   Insert

   T1head1-405(del37/GAG) (TLN1 Human)
   

   Use

   Tags

   His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…

   Expression

   Bacterial

   Mutation

   aa1-405, with 37 aa deletion in the F1-loop which…

   Promoter

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEN_TTmiRC2_3xflag_NRF2(RR)

   Plasmid

   #136522

   Purpose

   Used as a donor vector containing N-term 3xFLAG to clone into pSLIK

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   NRF2(1584A>C,1586A>T,1589A>G) (NFE2L2 Human)
   

   Use

   Entry vector for gateway cloning

   Tags

   3x FLAG

   Expression

   Mutation

   This plasmid is developed by mutating 3 base pair…

   Promoter

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. t1-405(151-154AAAA)

   Plasmid

   #166131

   Purpose

   Expression of human talin-1 head (aa1-405) in E. coli. Four substitutions in the F1-loop (aa151-154 all mutated to Al). N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site.

   Depositor

   Vesa Hytönen

   Article

   Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13

   Insert

   T1head1-405(151-154AAAA) (TLN1 Human)
   

   Use

   Tags

   His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…

   Expression

   Bacterial

   Mutation

   aa1-405 only. Contains 4 amino acid substitutions…

   Promoter

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLT3REVIR_MARK3 2573

   Plasmid

   #107233

   Purpose

   shRNA 2573. Do not use Evos or Acurri on these cells. Assess by FACS specifically with Venus and DsRed lazers. Tet-ON-all-in-one

   Depositor

   Alex Kentsis

   Article

   Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

   Insert

   MARK3 shRNA (22 nt) (MARK3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Ac5-STABLE2-RFP-NLS-CycB(1-266)_GFP-E2F1(1-230)_neo

   Plasmid

   #73164

   Purpose

   multi-cistronic Vector for expression for Fly_FUCCI probes in insect cells

   Depositor

   Bruce Edgar

   Article

   Zielke et al Cell Rep. 2014 Apr 24;7(2):588-98. doi:

   Insert

   mRFP-CycB(1-266)-T2A-GFP-E2F1(1-230)-T2A-neoR (E2f1, CycB Fly)
   

   Use

   Tags

   mRFP1, GFP

   Expression

   Insect

   Mutation

   Cyclin B Fragment aa 1-266 & E2F1 Fragment aa…

   Promoter

   Available since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs7903146-guide1

   Plasmid

   #125394

   Purpose

   CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs7903146 (TCF7L2 GWAS locus)

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. PG13-mCherry

   Plasmid

   #195291

   Purpose

   mCherry reporter containing 13 copies of the p53-binding consensus sequence

   Depositor

   Hideyuki Oguro

   Article

   Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PB-TO-hNGN2

    Plasmid

    #172115

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Article

    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

    Inserts

    hNGN2 (NEUROG2 Human)
    puroR-T2A-mycNLS-mTagBFP2
    

    Use

    Tags

    T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and TRE3G

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-PG13-mCherry-EF1a-PuroR-EGFP

    Plasmid

    #195292

    Purpose

    piggyBac transposon vector with mCherry reporter containing 13 copies of the p53-binding consensus sequence (constitutive puromycin resistance and EGFP)

    Depositor

    Hideyuki Oguro

    Article

    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

    Inserts

    mCherry
    PuroR-P2A-EGFP
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-syn-dLight1.3b (AAV9)

    Viral Prep

    #135762-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-syn-dLight1.3b (#135762). In addition to the viral particles, you will also receive purified pAAV-syn-dLight1.3b plasmid DNA. Syn-driven expression of dLight1.3b dopamine sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Promoter

    Syn

    Tags

    None

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

13. pAAV-CAG-dLight1.3b (AAV5)

    Viral Prep

    #125560-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-CAG-dLight1.3b (#125560). In addition to the viral particles, you will also receive purified pAAV-CAG-dLight1.3b plasmid DNA. CAG-driven expression of dLight1.3b dopamine sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Promoter

    CAG

    Tags

    None

    Available since

    Oct. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

14. pAAV-CAG-dLight1.3b (AAV1)

    Viral Prep

    #125560-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-CAG-dLight1.3b (#125560). In addition to the viral particles, you will also receive purified pAAV-CAG-dLight1.3b plasmid DNA. CAG-driven expression of dLight1.3b dopamine sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Promoter

    CAG

    Tags

    None

    Available since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

15. pAAV-CAG-dLight1.3b (AAV9)

    Viral Prep

    #125560-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-CAG-dLight1.3b (#125560). In addition to the viral particles, you will also receive purified pAAV-CAG-dLight1.3b plasmid DNA. CAG-driven expression of dLight1.3b dopamine sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Promoter

    CAG

    Tags

    None

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

16. pAAV-hSyn-dLight1.1 (AAV1)

    Viral Prep

    #111066-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-hSyn-dLight1.1 (#111066). In addition to the viral particles, you will also receive purified pAAV-hSyn-dLight1.1 plasmid DNA. hSyn-driven expression of dLight1.1 dopamine sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Promoter

    Tags

    None

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

17. pAAV-CAG-dLight1.2 (AAV1)

    Viral Prep

    #111069-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-CAG-dLight1.2 (#111069). In addition to the viral particles, you will also receive purified pAAV-CAG-dLight1.2 plasmid DNA. CAG-driven expression of dLight1.2 dopamine sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Promoter

    Tags

    None

    Available since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

18. pAAV-hSyn-dLight1.2 (AAV5)

    Viral Prep

    #111068-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-hSyn-dLight1.2 (#111068). In addition to the viral particles, you will also receive purified pAAV-hSyn-dLight1.2 plasmid DNA. Synapsin-driven expression of dLight1.2 dopamine sensor. This sensor has a slightly increased dynamic range and is better for 2P imaging in vivo. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Promoter

    Tags

    None

    Available since

    Dec. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

19. pAAV-CAG-dLight1.1 (AAV1)

    Viral Prep

    #111067-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-CAG-dLight1.1 (#111067). In addition to the viral particles, you will also receive purified pAAV-CAG-dLight1.1 plasmid DNA. CAG-driven expression of dLight1.1 dopamine sensor. This sensor is recommended for photometry in vivo. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Promoter

    Tags

    None

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

20. pAAV-CAG-dLight1.1 (AAV5)

    Viral Prep

    #111067-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-CAG-dLight1.1 (#111067). In addition to the viral particles, you will also receive purified pAAV-CAG-dLight1.1 plasmid DNA. CAG-driven expression of dLight1.1 dopamine sensor. This sensor is recommended for photometry in vivo. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Promoter

    Tags

    None

    Available since

    Dec. 14, 2018

    Availability

    Academic Institutions and Nonprofits only