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CS-TRE-BMI1-Ubc-rtTA

Plasmid

#198191

Purpose

Generation of Expandable megakaryocyte cell lines from human iPS cells

Depositor

Hiromitsu Nakauchi

Publication

Nakamura et al Cell Stem Cell. 2014 Apr 3;14(4):535-48.

Insert

hBMI1 (BMI1 Human)

Use

Lentiviral

Expression

Mammalian

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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HA-SOCS1

Plasmid

#174571

Purpose

Human HA-tagged SOCS1 expression (N-term. tag) (CMV prom.)

Depositor

Mathew Garnett

Publication

Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

Insert

HA-SOCS1

Expression

Mammalian

Promoter

CMV

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pEMS1375

Plasmid

#29176

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).

Depositor

Elizabeth Simpson

Publication

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple88 (GFAP Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available since

Nov. 14, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCR-Myc-IKKalpha

Plasmid

#19739

Depositor

Hiroyasu Nakano

Publication

Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

Insert

IkB kinase alpha (Chuk Mouse)

Tags

Myc

Expression

Mammalian

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pCAGGS-Stargazin-mEGFP-iLID

Plasmid

#178523

Purpose

Expresses Stargazin-mEGFP-iLID in mammalian cells

Depositor

Kazuhiro Aoki

Publication

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

Stargazin-mEGFP-iLID

Expression

Mammalian

Promoter

CAG

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCIG PIK3CA E545K

Plasmid

#73055

Purpose

Human PIK3CA E545K

Depositor

Joseph Gleeson

Publication

Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

Insert

PIK3CA (PIK3CA Human)

Expression

Mammalian

Mutation

E545K

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pF CAG luc IRES neo

Plasmid

#98294

Purpose

For constitutive expression of firefly luciferase from the CAG promoter together with an IRES-linked neo resistance gene

Depositor

Brett Stringer

Publication

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Inserts

Firefly luciferase
IRES
Neomycin resistance gene (NeoR)

Use

Lentiviral and Luciferase

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-GFP11(x7)-Actin

Plasmid

#181967

Purpose

Human beta actin tagged with 7 copies of GFP11.

Depositor

Jin Zhang

Publication

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

GFP11(x7)-Actin (ACTB )

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pME-QFGal4-SV40pA

Plasmid

#155118

Purpose

Middle entry vector containing QFGal4

Depositor

Bret Pearson

Publication

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QFGal4

Use

Gateway middle entry vector

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pSb_init containing anti SARS-CoV-2_RBD sybody Sb#42

Plasmid

#153525

Purpose

Expression of the anti SARS-CoV-2_RBD sybody Sb#42

Depositor

Markus Seeger

Publication

Walter et al bioRxiv 2020.04.16.045419

Insert

Sb#42

Expression

Bacterial

Promoter

pBAD

Available since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSH-EFIRES-P-Seipin-miniIAA7-mCherry

Plasmid

#129720

Purpose

AAVS1 safe harbor targeting vector expressing miniIAA7-mCherry tagged Seipin

Depositor

Elina Ikonen

Publication

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

Seipin (BSCL2 Human)

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

miniIAA7-mCherry

Expression

Mammalian

Mutation

silent mutations

Promoter

EF1a

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLQ-Pxyl/tet-cas9-Pj23119-sgRNA

Plasmid

#92121

Purpose

CRISPR-Cas9 based efficient genome editing in S. aureus

Depositor

Sheng Yang

Publication

Liu et al Acta Biochim Biophys Sin (Shanghai). 201

Insert

Cas9-Pxyltet-sgRNA-pj23119

Use

CRISPR

Expression

Bacterial

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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dmBACCS2-IRES-dOrai

Plasmid

#67627

Purpose

Mammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, and dOrai

Depositor

Takao Nakata

Publication

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

dmBACCS2-IRES-dOrai

Expression

Mammalian

Promoter

CMV

Available since

Aug. 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pClneoMyc human MOB1

Plasmid

#37024

Depositor

Yutaka Hata

Publication

Hirabayashi et al Oncogene. 2008 Jul 17;27(31):4281-92. Ep

Insert

MOB1B (MOB1B Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available since

May 23, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
p6604 MSCV-IP N-HAonly PP2CA

Plasmid

#35005

Depositor

Peter Howley

Publication

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

PP2CA (PP2CA Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available since

Feb. 8, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-NUP188

Plasmid

#159893

Purpose

Expresses NUP188 in mammalian cells

Depositor

Kozo Tanaka

Publication

Itoh et al Cancer Sci. 2013 Jul;104(7):871-9. doi:

Insert

NUP188 (NUP188 Human)

Tags

EGFP

Expression

Mammalian

Mutation

To avoid NotI site, first three amino acids are d…

Promoter

CMV

Available since

Oct. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_mitoSNAP

Plasmid

#136623

Purpose

Plasmid encoding outer mitochondrial localization SNAP-Tag

Depositor

Kai Johnsson

Publication

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag

Expression

Mammalian

Promoter

CMV

Available since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pME-tdTomato no stop

Plasmid

#82404

Purpose

Gateway-compatible tdTomato fusion reporter

Depositor

Judith Eisen

Publication

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

tdTomato (lacks stop codon)

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDEST_FUS_G2A_R521H

Plasmid

#26381

Depositor

Thomas Tuschl

Publication

Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

Insert

FUS (FUS Human)

Tags

FLAG-HA

Expression

Mammalian

Mutation

R521H

Available since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
pHPK1212

Plasmid

#8834

Depositor

David Waugh

Publication

Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

Insert

tobacco vein mottling virus protease, catalytic domain

Tags

MBP

Expression

Bacterial

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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