We narrowed to 36,794 results for: KAN

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Lck-FRB-ECFP(W66A)

Plasmid

#67902

Purpose

Expresses a membrane located FRB domain in mammalian cells (the ECFP is not fluorescent)

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

Lck-FRB-ECFP(W66A)

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pOPINK-VCPIP (OTU, aa 25-561)

Plasmid

#61583

Purpose

Expresses human VCPIP (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

VCPIP (VCPIP1 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-24 and aa 562-1222.

Promoter

T7

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-PLK4-S285A/T289A-3xFLAG

Plasmid

#69841

Purpose

This plasmid encodes PLK4 isoform 1 carrying S285A/T289A mutations with an N-terminal EGFP tag and C-terminal 3xFLAG tag

Depositor

Michel Bornens

Article

Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

Insert

PLK4 (PLK4 Human)

Tags

3xFLAG tag and EGFP

Expression

Mammalian

Mutation

S285A/T289A

Promoter

CMV

Available Since

Dec. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pRL1124

Plasmid

#70690

Purpose

provides avaiM, eco47iiM, and ecot22iM that encode specific methylases to enable transfer of plasmids from E. coli to Anabaena sp. strain PCC 7120 and other strains. lacks a mobilization function.

Depositor

Peter Wolk

Article

Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

Inserts

avaiM
eco47iiM
ecot22iM

Use

Bacterial shuttle vector

Available Since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pRL2960

Plasmid

#70694

Purpose

pDU1-E. coli shuttle vector (Km-r) with extended polylinker

Depositor

Peter Wolk

Article

Wolk et al Arch Microbiol. 2007 Dec;188(6):551-63.

Type

Empty backbone

Use

Bacterial shuttle vector

Available Since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP N1 Ub-CRT

Plasmid

#69619

Purpose

Express cytoplasmic mature CRT.

Depositor

Marta Hallak

Article

Goitea et al J Biol Chem. 2015 Jun 26;290(26):16403-1

Insert

Ubiquitina-Calreticulin (mature)

Tags

EGFP and Ubiquitin

Expression

Mammalian

Mutation

Deletion of signal peptide of calreticulin at the…

Promoter

CMV

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pL157A-EGFP

Plasmid

#68850

Purpose

mutated OPTN was inserted into pEGFP-N1 backbone

Depositor

Beatrice Yue

Article

Turturro et al Springerplus. 2014 Feb 19;3:99. doi: 10.

Insert

optineurin (OPTN Human)

Tags

EGFP

Expression

Mammalian

Mutation

L157A, mutation has not been linked to any diseas…

Promoter

CMV

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only
pECFP-C1-rGAT

Plasmid

#70108

Purpose

mammalian expression of rat GAT with N terminal CFP fusion

Depositor

Harald Sitte

Article

Scholze et al J Biol Chem. 2002 Nov 15;277(46):43682-9

Insert

GAT (Slc6a1 Rat)

Tags

ECFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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EBFP2-Cx43

Plasmid

#69020

Purpose

Expresses rat Cx43 (Gja1 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.

Depositor

David Spray

Article

Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

Insert

Cx43 (Gja1 Rat)

Tags

EBFP2

Promoter

CMV

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pVITRO1-SS-113

Plasmid

#68737

Purpose

ZF9-TF with mCherry marker

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Inserts

ZF9-VP64
mCherry

Tags

NLS and flag

Expression

Mammalian

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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4YWQ

Plasmid

#69953

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

RC3H1:YTC019-B07:C232791 (RC3H1 Human)

Tags

His and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO

Available Since

Oct. 7, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pDSHA-ARL8A-Myc

Plasmid

#67361

Purpose

Mammalian expression of hArl8 with C-term Myc tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8A (ARL8A Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARL8B

Plasmid

#67334

Purpose

hArl8b with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8B (ARL8B Human)

Use

Gateway Cloning

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-SAR1

Plasmid

#67344

Purpose

hSar1 with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SAR1 (SAR1A Human)

Use

Gateway Cloning

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
GK Rab3A Q81L p201

Plasmid

#44698

Depositor

Geri Kreitzer

Insert

Rab3A (RAB3A Human)

Use

Gateway Cloning

Mutation

Q81L

Available Since

Sept. 22, 2015

Availability

Academic Institutions and Nonprofits only
pBALU1

Plasmid

#69316

Purpose

Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in the 2nd intron of gfp

Depositor

Oliver Hobert

Article

Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/

Insert

GFPint-FgF

Expression

Bacterial

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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dmBACCS2NS-IRES-dOrai

Plasmid

#67628

Purpose

Mammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, and dOrai

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

dmBACCS2NS-IRES-dOrai

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2015

Availability

Academic Institutions and Nonprofits only

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attB-mCh-Poly(A)

Plasmid

#65525

Purpose

Plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attB-mCherry

Use

Mouse Targeting; Bxb1

Expression

Mammalian

Available Since

Aug. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pC210

Plasmid

#65950

Purpose

Weak Repressor plasmid for two-strain system

Depositor

Matthew Bennett

Article

Chen et al Science. 2015 Aug 28;349(6251):986-9. do

Insert

CinI

Tags

LAA tagged

Expression

Bacterial

Promoter

Prhl/lac-w

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pC165

Plasmid

#65949

Purpose

P1 activator plasmid for two-strain system

Depositor

Matthew Bennett

Article

Chen et al Science. 2015 Aug 28;349(6251):986-9. do

Insert

RhlI (rhlI )

Tags

LAA tagged

Expression

Bacterial

Promoter

pLLac*

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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