We narrowed to 43,849 results for: tro
-
Plasmid#183924PurposeVPR activation domain coupled to catalytically inactive dCas9 for localization; contains a GFP tetramer fpr labeling and as a spacerDepositorInsertdCas9-GFP4-VPR
UseCRISPRTagsNLSExpressionMammalianMutationnonePromoterCMVAvailable SinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGfa28-nLac
Plasmid#53130PurposeExpression of LacZ driven by partial human gfa2 promoter, expression is localized to a subset of astrocytes in mice.DepositorInsertGfa2 (GFAP Human)
UseMouse TargetingTagsLacZ, NLS, and mP1ExpressionMammalianMutationcontains the A and B regions (bp -1757 to -1489) …Promoterhuman Gfa2Available SinceJuly 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0107
Plasmid#177040PurposeMoClo Level 1, position 1, transcriptional unit for transient expression of 1-deoxy-D-xylulose 5-phosphate synthase (CrDXS2) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptideDepositorInsert1-deoxy-D-xylulose 5-phosphate synthase (CrDXS2) from Catharanthus roseus
UseSynthetic BiologyAvailable SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0202
Plasmid#185626PurposeMoClo Level 1, position 5 reverse, transcriptional unit for expression of plant codon optimized Cas9 from Streptococcus pyogenes driven by 35S promoterDepositorInsertSpCas9
UseCRISPR and Synthetic BiologyAvailable SinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAA3
Plasmid#154163PurposeFirefly luciferase under the control of 5 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.DepositorInsertFirefly luciferase
UseLuciferaseMutation20 wt NF-κB sites were replaced with Rel1A-specif…Available SinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX330_rSyn1 sgRNA / hSpCas9
Plasmid#172840PurposeMammalian expression of a sgRNA targeting the intron 21 (last intron) of rSyn1 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 21 of rSyn1 under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB80-SSPB(micro)-GFP-ppKin14VIb(861-1321)
Plasmid#174639PurposeOptogenetic coupling to dimeric moss kinesin-14 for retrograde transport via SSPB(micro)DepositorInsertSSPB(micro)-GFP-ppKinVIb(861-1321)
TagsSSPB(micro)-GFPExpressionMammalianMutationSSPB:Arg73Gln; EGFP: Met1Del; ppKin14VIb(aa861-13…PromoterChicken beta-actinAvailable SinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST myc MOV10 K530A
Plasmid#51719Purposeplasmid expresses myc-tagged MOV10 K530ADepositorAvailable SinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDRF’- AmTryoshka1;3-GS
Plasmid#100967PurposeYeast expression of control vector for fluorescent sensors reporting the activity of ammonium transceptors.DepositorAvailable SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
Opto-casp9_casp9-VfAU1-LOV
Plasmid#131222PurposeExpresses Opto-casp9_casp9-VfAU1-LOV (light-activated caspase9)DepositorInsertOpto-casp9 (casp9-VfAU1-LOV)
ExpressionMammalianPromoterCMVAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only