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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,451 results for: grn

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Showing: 11261 - 11280 of 15451 results

  4. Mammalian_SaAID3S

    Plasmid
    #188651
    Purpose
    Expresses SaAID3S and gRNA in mammalian cells
    Depositor
    Keiji Nishida
    Publication
    Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    nSaCas9-AID3S--UGI
    Sa-sgRNA
    Tags
    UGI
    Expression
    Mammalian
    Mutation
    D10A for SaCas9
    Promoter
    EF-1a and H1
    Available since
    Oct. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188651-plasmid-map-sequence-id-375883

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  5. Mammalian_SaAID2S

    Plasmid
    #188652
    Purpose
    Expresses SaAID2S and gRNA in mammalian cells
    Depositor
    Keiji Nishida
    Publication
    Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    AID2S-nSaCas9--UGI
    Sa-sgRNA
    Tags
    UGI
    Expression
    Mammalian
    Mutation
    D10A for SaCas9
    Promoter
    EF-1a and H1
    Available since
    Oct. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188652-plasmid-map-sequence-id-375885

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  6. pSLiP-G2-RacE

    Plasmid
    #188966
    Purpose
    L-Rhamnose inducible catalytically inactive Cas9 with sgRNA and racE gene for bacterial gene knockdown
    Depositor
    Seung-Goo Lee
    Publication
    Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131
    Inserts
    dCas9
    sgRNA: gttagacgctgattacatggactagg
    racE
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    PrhaBAD
    Available since
    Sept. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    188966-plasmid-map-sequence-id-371637

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  7. Phage-PGK-FLAG-HA-T(WT)

    Plasmid
    #181737
    Purpose
    for lentiviral expression of brachyury ORF in mammalian cells
    Depositor
    Charles Lin
    Insert
    T (brachyury)
    Use
    Lentiviral
    Mutation
    a silent mutation in the PAM site of an sgRNAt ta…
    Available since
    Aug. 3, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    181737-plasmid-map-sequence-id-357225

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  8. pX459-puro-hPKAalpha

    Plasmid
    #185379
    Purpose
    For mammalian expression of guide RNA: caccgTTTGAACGAATCAAGACCCT that targets human PKA subunit alpha
    Depositor
    Ayaz Najafov
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PRKACA (PRKACA Human)
    Expression
    Mammalian
    Mutation
    WT
    Available since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    185379-plasmid-map-sequence-id-363450

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  9. pX459-puro-hGSDMD

    Plasmid
    #185377
    Purpose
    For mammalian expression of guide RNA: CGCGCCAGACGCGCCACCCT that targets human GSDMD (Gasdermin D)
    Depositor
    Ayaz Najafov
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GSDMD (GSDMD Human)
    Expression
    Mammalian
    Mutation
    WT
    Available since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    185377-plasmid-map-sequence-id-363446

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  12. cTRE-LSL-GFP-IRES-Cas9-sgCR8

    Plasmid
    #135668
    Purpose
    Introduce Dox/Cre-inducible (TRE promoter followed by lox-stop-lox) Cas9 cDNA plus control-targeting sgRNA into (ES) cells by recombination-mediated cassette exchange
    Depositor
    Florian Karreth
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgCR8
    Use
    Mouse Targeting
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135668-plasmid-map-sequence-id-325838

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  13. pJK429

    Plasmid
    #155376
    Purpose
    PphlF_A4NT in dCRv1, 1x cascade + [dCas9 + PA4-mVenus]
    Depositor
    Pamela Silver
    Publication
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas9 (bacteria)
    PA4-mVenus
    PphlF_A4NT in dCas9 sgRNA oscillator v1
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D10A H840A (catalytically inactive)
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    155376-plasmid-map-sequence-id-308997

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  16. pLKO.puro_shHuR 3UTR

    Plasmid
    #110414
    Purpose
    TRCN0000276186 (Target TTGTTAGTGTACAACTCATTT), silence human ELAVL1 (HuR) gene, puromycin selection
    Depositor
    Sandra Martha Gomes Dias
    Insert
    ELAVL1 (HuR)
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    U6 (RNA PolIII)
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    110414-plasmid-map-sequence-id-213427

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  17. pMY-CD90.2-Rluc_miR

    Plasmid
    #163333
    Purpose
    Retroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a CD90.2 surface marker under control of the LTR promoter.
    Depositor
    Louis Ates, Teunis Geijtenbeek
    Publication
    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.
    Insert
    CD90.2 (Thy1 Mouse)
    Use
    RNAi and Retroviral
    Expression
    Mammalian
    Promoter
    LTR
    Available since
    Jan. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    163333-plasmid-map-sequence-id-322028

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Showing: 11261 - 11280 of 15451 results