We narrowed to 31,412 results for: PLE
-
Plasmid#114632PurposeAddgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAPDepositorInsertdCas9-omega
UseCRISPRExpressionBacterialPromoterTet promoterAvailable SinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJAT71
Plasmid#204305PurposeExpresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable ie1XP3-DsRed cassette expressed in abdomen and mouthparts.DepositorInsertie1-DsRed
UseCRISPRPromoterie1Available SinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sapphireCKAR(T/A)
Plasmid#118469PurposeNegative-control mutant for sapphireCKAR biosensor.DepositorInsertsapphireCKAR(T/A)
Tags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable SinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
C413-E45: attB4-hr5-ie1p>-attB1r
Plasmid#162930PurposeGateway attB4/attB1r entry clone for transient insect expression using the ie1 promoter from AcMNPVDepositorInsertAcMNPV ie1 promoter with hr5 enhancer
UseSynthetic BiologyAvailable SinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
PM-ABKAR
Plasmid#61506PurposeExpression of AMPK biosensor targeted to the plasma membrane via Lyn kinase targeting sequenceDepositorInsertAMPK Biosensor
TagsLyn kinase sequence (see comments)ExpressionMammalianPromoterCMVAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pC0057 BzoCas13 (B01) His6-TwinStrep-SUMO-BsaI
Plasmid#115207PurposeExpresses BzoCas13b from a SUMO-tagged bacterial expression constructDepositorInsertBzoCas13
TagsSUMOExpressionBacterialAvailable SinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RAB-EKARev(T/A)
Plasmid#118475PurposeNegative-control mutant for RAB-EKARev biosensor.DepositorInsertRAB-EKARev(T/A)
Tags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag TSC2 (T1462A)
Plasmid#14130DepositorAvailable SinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcFLAG RUNX3 (R122C)
Plasmid#203438PurposeMammalian expression of RUNX3DepositorAvailable SinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSW149
Plasmid#122248PurposepTYB2 containing TIF4631 gene, expresses yeast eIF4G1 as an intein-chitin-binding domain fusion for purification of untagged full-length protein, with or without a fluorescent label on the C-terminus.DepositorInsertTIF4631 (TIF4631 Budding Yeast)
TagsIntein-Chitin-binding domainExpressionBacterialPromoterT7Available SinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1 hBRM
Plasmid#1956DepositorAvailable SinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV14-mULK1
Plasmid#24301DepositorInserta novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)
Tags3xFLAGExpressionMammalianAvailable SinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pscALPS-HIV-1 Group P-vpr
Plasmid#115837PurposeEncodes codon optimized 3xFLAG-HIV1 Group P Vpr (HQ179987) and puromycin resistance proteinDepositorInsertHIV1 Group P Vpr (HQ179987)
UseLentiviralTags3x-FLAGPromoterSFFVAvailable SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR MCS L1-L4
Plasmid#62087PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a multiple cloning site. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseMule gateway entry vectorExpressionMammalianAvailable SinceFeb. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
ePPEmax*
Plasmid#205244PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertc-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
UseCRISPRExpressionPlantMutationDetailed in manuscriptAvailable SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-Skp2
Plasmid#19946DepositorAvailable SinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
SPDK3860
Plasmid#199245PurposeModified Tobacco rattle virus (TRV) RNA2 vector for editing of Nicotiana benthamiana Phytoene desaturase (PDS) geneDepositorInsertModified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence
UseT-dna vectorPromoterpPEBVAvailable SinceJune 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
(SIM)5
Plasmid#126947PurposeBacterial expression plasmid for (SIM)5 client to assess SUMO-SIM scaffolds.DepositorInsert5 repeats of SUMO interacting motif (SIM) from PIASx, each separated by (GGS)4 (PIAS2 Human)
ExpressionBacterialPromoterunknownAvailable SinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGCP123-GFP_g1
Plasmid#153518PurposesgRNA to GFP gene (NT) under nisin-inducible nisA promoter, barcodedDepositorInsertPnisA-sgRNA(GFP_g1)_dCas9 scaffold (barcoded)
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-BIRC3-SU
Plasmid#160699PurposeExpresses GFP-BIRC3 with the short 3'UTR in mammalian cellsDepositorInsertGFP-BIRC3 (BIRC3 Human)
ExpressionMammalianAvailable SinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only