We narrowed to 11,615 results for: Vars;
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Plasmid#125403PurposeCRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs182222907 (C2CD4A/B GWAS locus)DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianPromoterhPGK and U6Available SinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4AB-enh1-guide2
Plasmid#125401PurposeCRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs182222907 (C2CD4A/B GWAS locus)DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianPromoterhPGK and U6Available SinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs4237150-guide4
Plasmid#125399PurposeCRISPR-mediated repression of human islet enhancer containing T1D/T2D-associated variant rs4237150 (GLIS3 GWAS locus)DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianPromoterhPGK and U6Available SinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs4237150-guide3
Plasmid#125398PurposeCRISPR-mediated repression of human islet enhancer containing T1D/T2D-associated variant rs4237150 (GLIS3 GWAS locus)DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianPromoterhPGK and U6Available SinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs4237150-guide1
Plasmid#125397PurposeCRISPR-mediated repression of human islet enhancer containing T1D/T2D-associated variant rs4237150 (GLIS3 GWAS locus)DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianPromoterhPGK and U6Available SinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs11257655-guide1
Plasmid#125393PurposeCRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus)DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianPromoterhPGK and U6Available SinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4AB-enh1-guide1
Plasmid#125400PurposeCRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs182222907 (C2CD4A/B GWAS locus)DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianPromoterhPGK and U6Available SinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
P931_CLYBL-CBX3-Cbh-Zeo-TetO-NAIP2
Plasmid#202761PurposeEncodes four RGC specific transcription factors under the control of a TetOn promoter at the CLYBL safe harborDepositorExpressionMammalianPromoterTetOAvailable SinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-N_PWP1:1-501
Plasmid#210974PurposeMammalian expression of full-length human PWP1. N-terminal NanoLuc luciferase tag.DepositorInsertPWP1:M1-S501 (PWP1 Human)
TagsNanoLuc luciferaseExpressionMammalianMutationE165D F448L G449R not annotated variantsPromoterCMVAvailable SinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_PWP1:1-501
Plasmid#211189PurposeMammalian expression of full-length human PWP1. N-terminal HiBiT tag.DepositorInsertPWP1:M1-S501 (PWP1 Human)
TagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationE165D F448L G449R not annotated variantsPromoterCMVAvailable SinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-C_PWP1:1-501
Plasmid#211262PurposeMammalian expression of full-length human PWP1. C-terminal HiBiT tag.DepositorInsertPWP1:M1-S501 (PWP1 Human)
TagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationE165D F448L G449R not annotated variantsPromoterCMVAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-C_PWP1:1-501
Plasmid#210975PurposeMammalian expression of full-length human PWP1. C-terminal NanoLuc luciferase tag.DepositorInsertPWP1:M1-S501 (PWP1 Human)
TagsNanoLuc luciferaseExpressionMammalianMutationE165D F448L G449R not annotated variantsPromoterCMVAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGCS vector kit
Plasmid Kit#1000000107PurposeA series of pCS2+ based Gateway vectors with N- or C-terminal tags (eg Myc, HA, Flag, GST and eGFP), which allow the generation of tagged proteins in a variety of vertebrate organisms.DepositorAvailable SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
FX cloning Kit
Plasmid Kit#1000000039PurposeInsert sequence of interest into a cloning vector and subclone into expression vectors, containing a variety of N- and C-terminal tags; for use in both prokaryotic and eukaryotic systemsDepositorAvailable SinceApril 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
CRISPRecise Kit
Plasmid Kit#1000000235PurposeCRISPRecise is a set of high-fidelity SpCas9 variants that can be used to efficiently edit any SpCas9 target with practically no detectable off-target effects.DepositorAvailable SinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
Platinum Gate TALEN Kit
Plasmid Kit#1000000043PurposeEnables construction of highly-active Platinum TALENs using two-step Golden Gate cloning method. Platinum TALENs have variable TALE repeats with either +136/+63 or +153/+47 TALE scaffolds.DepositorAvailable SinceMay 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
CIDAR MoClo Extension, Volume I
Plasmid Kit#1000000161PurposeThis kit contains 110 plasmids designed to quickly and efficiently build a wide variety of gene expression constructs using the CIDAR-standard MoClo system.DepositorAvailable SinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
EcoFlex MoClo Kit
Plasmid Kit#1000000080PurposeGolden Gate kit for E. coli synthetic biology; consists of a modular library that uses MoClo assembly standard for a variety of uses, e.g. recombinant protein purification, cell-free circuit designDepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
EMMA toolkit
Plasmid Kit#1000000119PurposeUsed for the construction of a variety of mammalian expression vectors in a one-pot, one-step Golden Gate reaction; compatible with automated productionDepositorAvailable SinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
EcoFlex MoClo Extension, Volume I
Plasmid Kit#1000000253PurposeUse the EcoFlex extension kit (volume one) to quickly and efficiently build a wide variety of gene expression constructs using the EcoFlex-standard MoClo system.DepositorAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only