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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 79,231 results for: rest

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Showing: 11321 - 11340 of 79231 results

  1. VAMP8-mCherry

    Plasmid
    #92424
    Purpose
    Expression of VAMP8 C-terminally conjugated to mCherry.
    Depositor
    Geert van den Bogaart
    Publication
    Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    VAMP8 (Vamp8 Mouse)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    A36S (Please see Depositor Comments below)
    Promoter
    CMV
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92424-plasmid-map-sequence-id-182516

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  2. pAc5 HA3-eDHFR-T2A-puro

    Plasmid
    #86395
    Purpose
    for C-terminal tagging with eDHFR trimethoprim regulated degron with puro selection
    Depositor
    David Bentley
    Publication
    Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HA3 eDHFR T2A puro
    Use
    CRISPR
    Tags
    HA3 eDHFR T2A puro
    Mutation
    R12Y, G67S and Y100I mutations in E coli DHFR
    Available since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    86395-plasmid-map-sequence-id-170910

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  5. pAAV Syn ChR E90R-T159C 2A tDimer

    Plasmid
    #52495
    Purpose
    Historical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)
    Depositor
    Thomas Oertner
    Publication
    Wietek et al Science. 2014 Mar 27.
    Inserts
    Channelrhodopsin-2
    red fluorescent protein
    Use
    AAV
    Expression
    Mammalian
    Mutation
    E90R, T159C
    Promoter
    human synapsin-1 and ribosomal skip sequence
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    52495-plasmid-map-sequence-id-81430

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  13. K785R Smarca4-sfGFP

    Plasmid
    #107057
    Purpose
    Expression of dominant-negative K785R Smarca4 (Brg1) with C-terminal super-folder GFP fusion.
    Depositor
    Jerry Crabtree, Courtney Hodges
    Publication
    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7
    Insert
    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    Use
    Lentiviral
    Tags
    Super-folder GFP (sfGFP)
    Expression
    Mammalian
    Mutation
    K785R
    Promoter
    CAG
    Available since
    April 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    107057-plasmid-map-sequence-id-201959

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  14. CMV-Cas13Y.1-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA

    Plasmid
    #171381
    Purpose
    Expression vector for encoding a human codon-optimized Cas13Y.1 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.
    Depositor
    Hui Yang
    Publication
    Xu et al Nat Methods. 2021 May;18(5):499-506. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized Cas13Y.1
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    CMV, U6
    Available since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    171381-plasmid-map-sequence-id-337347

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  17. eSpCas9(1.1)-Puro

    Plasmid
    #117686
    Purpose
    The plasmids pSpCas9 (BB)-2A-Puro (Addgene, #48139) and eSpCas9 (1.1) (Addgene, #71814) were combined to express both “enhanced specificity” Cas9 and puromycin resistance protein.
    Depositor
    Luc Leyns
    Publication
    Liu et al Biochem Biophys Res Commun. 2019 Feb 14.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eSpCas9(1.1)
    Use
    CRISPR
    Tags
    PuroR (R166H)
    Expression
    Mammalian
    Mutation
    K848A, K1003A, R1060A
    Promoter
    U6
    Available since
    Feb. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    117686-plasmid-map-sequence-id-236350

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  20. EGFP-Rab8a-QL

    Plasmid
    #86076
    Purpose
    A mammalian expression plasmid encoding Rab8a GTPase-deficient mutant (Rab8a-Q67L) with N-terminus EGFP.
    Depositor
    Lei Lu
    Publication
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hRab8A (RAB8A Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Changed glutamine 67 to leucine
    Promoter
    CMV
    Available since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    86076-plasmid-map-sequence-id-168965

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Showing: 11321 - 11340 of 79231 results