Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 14,060 results for: crispr grnas

Clear keywords
Showing: 11341 - 11360 of 14060 results

  4. pSCL.040

    Plasmid
    #184974
    Purpose
    Test effect of extending a1/a2 on ADE2 editing rates in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 27 v2
    Expression
    Yeast
    Mutation
    ADE2 donor P272stop, a1/a2 length extended to 27 …
    Promoter
    Gal7
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184974-plasmid-map-sequence-id-376705

    Enlarge

  5. pSCL.109

    Plasmid
    #184978
    Purpose
    Test effect of extending a1/a2 on LYP1 editing rates in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 editing ncRNA and gRNA, LYP1 E27X, a1/a2 length: 27 v1
    Expression
    Yeast
    Mutation
    LYP1 donor E27stop, a1/a2 length extended to 27 bp
    Promoter
    Gal7
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184978-plasmid-map-sequence-id-376679

    Enlarge

  6. pSCL.107

    Plasmid
    #184976
    Purpose
    Test effect of extending a1/a2 on CAN1 editing rates in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 editing ncRNA and gRNA, CAN1 G444X, a1/a2 length: 27 v1
    Expression
    Yeast
    Mutation
    CAN1 donor G444stop, a1/a2 length extended to 27 …
    Promoter
    Gal7
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184976-plasmid-map-sequence-id-376676

    Enlarge

  7. pSCL.111

    Plasmid
    #184980
    Purpose
    Test effect of extending a1/a2 on TRP2 editing rates in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 editing ncRNA and gRNA, TRP2 E64X, a1/a2 length: 27 v1
    Expression
    Yeast
    Mutation
    TRP2 donor E64stop, a1/a2 length extended to 27 bp
    Promoter
    Gal7
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184980-plasmid-map-sequence-id-376669

    Enlarge

  8. pSCL.113

    Plasmid
    #184982
    Purpose
    Test effect of extending a1/a2 on FAA1 editing rates in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 editing ncRNA and gRNA, FAA1 P233X, a1/a2 length: 27 v1
    Expression
    Yeast
    Mutation
    FAA1 donor P233stop, a1/a2 length extended to 27 …
    Promoter
    Gal7
    Available Since
    Nov. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    184982-plasmid-map-sequence-id-376532

    Enlarge

  9. pSCL.039

    Plasmid
    #184973
    Purpose
    Test effect of extending a1/a2 on ADE2 editing rates in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 27 v1
    Expression
    Yeast
    Mutation
    ADE2 donor P272stop, a1/a2 length extended to 27 …
    Promoter
    Gal7
    Available Since
    Nov. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    184973-plasmid-map-sequence-id-376529

    Enlarge

  10. pJEC819

    Plasmid
    #190815
    Purpose
    CRISPRa backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.
    Depositor
    James Chappell
    Article
    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    dCas9-XTEN-rpoA
    Use
    CRISPR and Synthetic Biology
    Tags
    XTEN (linker), followed by the N-terminal domain …
    Promoter
    SP30
    Available Since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    190815-plasmid-map-sequence-id-373717

    Enlarge

  11. pX330-BsaIx2-DD-Cas9

    Plasmid
    #75380
    Purpose
    Expresses DD-SpCas9 in mammalian cells and has a cloning site for an sgRNA. The FKBP12 L106P destabilization domain allows for inducible stabilization of Cas9 through the addition of Shield-1
    Depositor
    Michele Calos
    Article
    Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv
    Insert
    DD-SpCas9
    Use
    CRISPR
    Tags
    FKBP12 L106P DD
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    75380-plasmid-map-sequence-id-192102

    Enlarge

  12. pJEC726

    Plasmid
    #190814
    Purpose
    CRISPRi backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.
    Depositor
    James Chappell
    Article
    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    dCas9
    Use
    CRISPR and Synthetic Biology
    Mutation
    S. pyogenes dCas9 codon optimized for Streptomyce…
    Promoter
    SP30
    Available Since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    190814-plasmid-map-sequence-id-373712

    Enlarge

  13. pRhaCAST

    Plasmid
    #211791
    Purpose
    pZLrhaB2plus with Sh-cas12K-tnsBC-tniQ cloned into, which drives by the rhamnose inducible promoter
    Depositor
    Silvia Cardona
    Article
    Yap et al Appl Environ Microbiol. 2024 Jul 24;90(7
    Insert
    ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    Rhamnose-inducible
    Available Since
    June 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    211791-plasmid-map-sequence-id-418380

    Enlarge

  14. pGES201

    Plasmid
    #190197
    Purpose
    For CRISPR-Cas9 mediated gene editing in soybean, soybean elongation factor 1A promoter (pM4)-Cas9, GmU6-sgRNA, Basta selection
    Depositor
    Yuefeng Guan
    Article
    Bai et al Plant Biotechnol J. 2020 Mar;18(3):721-7
    Insert
    spCas9
    Use
    CRISPR
    Tags
    3xFlag
    Expression
    Plant
    Mutation
    plant-codon optimized
    Promoter
    Glycine max elongation factor 1A(pM4)
    Available Since
    Sept. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    190197-plasmid-map-sequence-id-371938

    Enlarge

Showing: 11341 - 11360 of 14060 results