We narrowed to 32,790 results for: fer

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tdTomato-β-actin3'UTR1

Plasmid

#183716

Purpose

tdTomato with fragment nucleotides 1-440 of beta-actin 3'UTR inserted in 3'UTR

Depositor

Jeffrey Gerst

Article

Dasgupta et al Elife. 2023 May 30;12:e83584. doi: 10.75

Insert

tdTomato with fragment (nucleotides 1-440) of mouse Actb 3'UTR

Use

Lentiviral

Promoter

UbiC

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV_Hyg_FLAG-BirA-eGFP-NLS2

Plasmid

#170917

Purpose

Expresses FLAG-BirA-eGFP-NLS2 to be used as control in BioID or FLAG pull down

Depositor

Constance Ciaudo

Article

Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

Insert

FLAG-BirA-eGFP-NLS2

Tags

BirA-FLAG

Expression

Mammalian

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19_HR-BirA-FLAG_Ago2

Plasmid

#170918

Purpose

Encodes BirA-FLAG with left and right homology arms for endogenous tagging of Ago2

Depositor

Constance Ciaudo

Article

Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

Insert

BirA-FLAG

Expression

Mammalian

Promoter

lac

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19_HR-BirA-FLAG_Ago1

Plasmid

#170919

Purpose

Encodes BirA-FLAG with left and right homology arms for endogenous tagging of Ago1

Depositor

Constance Ciaudo

Article

Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

Insert

BirA-FLAG

Expression

Mammalian

Promoter

lac

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-Ub-VPS13D

Plasmid

#176462

Purpose

expressing GFP-Ub marked VPS13D in mammalian cells. GFP-Ub will be cleaved by DUBs to express untagged VPS13D

Depositor

Richard Youle

Article

Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

VPS13D (VPS13D Human)

Tags

GFP-Ub

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-Ub-VPS13D N3521S

Plasmid

#176463

Purpose

expressing VPS13D patient mutant N3521S in mammalian cells

Depositor

Richard Youle

Article

Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

VPS13D (VPS13D Human)

Tags

GFP-Ub

Expression

Mammalian

Mutation

N3521S mutation

Promoter

CMV

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pPLN-hFluc-WT-eGFP-KDEL

Plasmid

#177727

Purpose

Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention and fused to eGFP at the C-terminus

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

hFluc

Tags

KDEL, Prolactin Signal Sequence, and eGFP

Expression

Bacterial and Mammalian

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0911

Plasmid

#177067

Purpose

MoClo Level 1, position 5, transcriptional unit for transient expression of loganic acid O-methyltransferase (MsLAMT) from Mitragyna speciosa driven by 35S promoter

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

loganic acid O-methyltransferase (MsLAMT) from Mitragyna speciosa

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0769

Plasmid

#177059

Purpose

MoClo Level 1, position 5, transcriptional unit for transient expression of loganic acid O-methyltransferase (CrLAMT) from Catharanthus roseus driven by 35S promoter

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

loganic acid O-methyltransferase (CrLAMT) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0123

Plasmid

#177055

Purpose

MoClo Level 1, position 3, transcriptional unit for transient expression of 7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus driven by 35S promoter

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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OA-1010Av2

Plasmid

#176082

Purpose

pBac-10xUAS-L596_g9608-p10-UTR-Opie2-dsRed

Depositor

Omar Akbari

Article

Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

Insert

L596_g9608.t1

Tags

3xHA

Expression

Bacterial

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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OA-1010Bv2

Plasmid

#176083

Purpose

pBac-10xUAS-L596_g25050-p10-UTR-Opie2-dsRed

Depositor

Omar Akbari

Article

Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

Insert

L596_g25050.t1

Tags

3xHA

Expression

Bacterial

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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6xHis-CPL2/pET28a

Plasmid

#172095

Purpose

cDNA of CER11/CPL2 cloned into pET28a for protein expression

Depositor

George Haughn

Article

Shi et al Plant Physiol. 2019 Nov;181(3):901-915.

Insert

CPL2 (CPL2 Mustard Weed)

Tags

6x His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CPL2g/pDONR221

Plasmid

#172093

Purpose

Genomic fragment containing the upstream region and coding region of CER11/CPL2 cloned into pDONR221

Depositor

George Haughn

Article

Shi et al Plant Physiol. 2019 Nov;181(3):901-915.

Insert

CPL2 (CPL2 Mustard Weed)

Use

Gateway entry vector

Promoter

CPL2

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pTVKRSF224

Plasmid

#135004

Purpose

MusF1 prenyltransferases from Nmu PCC 7906

Depositor

Hideo Iwai

Article

Mattila et al ACS Chem Biol. 2019 Dec 20;14(12):2683-2

Insert

MusF1 prenyltransferases

Expression

Bacterial

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pTVKRSF225

Plasmid

#135005

Purpose

MusF2 prenyltransferases from Nmu. PCC 7906

Depositor

Hideo Iwai

Article

Mattila et al ACS Chem Biol. 2019 Dec 20;14(12):2683-2

Insert

MusF2 prenyltransferaseM

Tags

SUMO fusion

Expression

Bacterial

Promoter

T7

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pBHRSF227

Plasmid

#135006

Purpose

MusF1 prenyltransferase from Nostoc sp. UHCC 0398

Depositor

Hideo Iwai

Article

Mattila et al ACS Chem Biol. 2019 Dec 20;14(12):2683-2

Insert

MusF1 prenyltransferase

Tags

SUMO fusion

Expression

Bacterial

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pRV∆G-N*PEST-4Cre

Plasmid

#174381

Purpose

Rabies vector for expressing Cre recombinase and N gene with a stop codon before PEST domain

Depositor

Ian Wickersham

Article

Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

Insert

Cre recombinase

Expression

Mammalian

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pET28-sfGFP-DogTag Loop C

Plasmid

#171781

Purpose

Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Asp173 and Gly174

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

sfGFP-DogTag Loop C

Tags

His6

Expression

Bacterial

Mutation

DogTag and linkers inserted between residues Asp1…

Promoter

T7

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pET28-sfGFP-SpyTag003 Loop B

Plasmid

#171777

Purpose

Expresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Asp102 and Asp103

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

sfGFP-SpyTag003 Loop B

Tags

His6

Expression

Bacterial

Mutation

SpyTag003 and linkers inserted between residues A…

Promoter

T7

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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