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pCAGGS-3HA-Pik3r1[114-313]

Plasmid

#246249

Purpose

Rab4 GAP

Depositor

Ryohei Yasuda

Article

Wang et al Elife. 2025 Sep 23;13:RP103879. doi: 10.

Insert

3HA-Pik3r1[114-313] (Pik3r1 Rat)

Tags

3HA

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-3HA-Rab3gap1

Plasmid

#246278

Purpose

Rab10 GAP

Depositor

Ryohei Yasuda

Article

Wang et al Elife. 2025 Sep 23;13:RP103879. doi: 10.

Insert

3HA-Rab3gap1 (Rab3gap1 Mouse)

Tags

3HA

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_2_064_Synthetic_promoter_08

Plasmid

#235817

Purpose

Synthetic promoter 08 based on Chlamydomonas rrn16 promoter

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

Synthetic promoter 08

Use

Synthetic Biology

Mutation

none

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_3IEE_023_IEE5

Plasmid

#235869

Purpose

tscA-chlN IEE5 Chlamydomonas reinhardtii

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

IEE5

Use

Synthetic Biology

Mutation

none

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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hVAMP3(1-77) WT

Plasmid

#206028

Purpose

Expresses the SNARE motif of wildtype human VAMP3 in bacteria

Depositor

Geert van den Bogaart

Article

Chen et al J Cell Sci. 2025 Sep 22:jcs.264139. doi:

Insert

Vesicle-associated membrane protein 3 (VAMP3 Human)

Tags

his-tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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hVAMP3(1-77) S44A

Plasmid

#206029

Purpose

Expresses the SNARE motif of human VAMP3 mutant S44A in bacteria

Depositor

Geert van den Bogaart

Article

Chen et al J Cell Sci. 2025 Sep 22:jcs.264139. doi:

Insert

Vesicle-associated membrane protein 3 (VAMP3 Human)

Tags

his-tag

Expression

Bacterial

Mutation

changes serine 44 to alanine

Promoter

T7

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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hVAMP3(1-77) S44E

Plasmid

#206030

Purpose

Expresses the SNARE motif of human VAMP3 mutant S44E in bacteria

Depositor

Geert van den Bogaart

Article

Chen et al J Cell Sci. 2025 Sep 22:jcs.264139. doi:

Insert

Vesicle-associated membrane protein 3 (VAMP3 Human)

Tags

his-tag

Expression

Bacterial

Mutation

changes serine 44 to glutamic acid

Promoter

T7

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pET15b His SUMO mNeon Δ37 PPM1H

Plasmid

#240065

Purpose

Expresses His SUMO mNeon (inserted in loop1) Δ37 PPM1H in bacterial cells

Depositor

Suzanne Pfeffer

Article

Adhikari et al J Biol Chem. 2025 Sep 3:110679. doi: 10.

Insert

His SUMO mNeon (loop 1) Δ37 PPM1H (PPM1H Human)

Expression

Bacterial

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.1 mouse NT

Plasmid

#242421

Purpose

Expression of a non-targeting shRNA in mouse cells

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

Non-targeting shRNA

Expression

Mammalian

Promoter

U6

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLX-TRV2-vCHLI

Plasmid

#239842

Purpose

Derivative of pLX-TRV2, a tobacco rattle virus (TRV) vector, designed for the expression of a 32-nt insert to silence CHLI homolog genes in Nicotiana benthamiana and Solanum aethiopicum

Depositor

Fabio Pasin

Article

Garcia et al Plant Biotechnol J. 2025 Nov;23(11):4930

Insert

vCHLI

Use

RNAi

Expression

Plant

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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SpRY-IP

Plasmid

#235994

Purpose

Expresses Cas9-SpRY in mammalian cells

Depositor

Tobias Cantz

Article

Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

SpRY

Use

CRISPR

Expression

Mammalian

Promoter

EF-1a

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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iSM-IP

Plasmid

#235990

Purpose

Expresses iSM (iSpyMac) in mammalian cells

Depositor

Tobias Cantz

Article

Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

iSM

Use

CRISPR

Expression

Mammalian

Promoter

EF-1a

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pET50del_SynPspA_R44K/E126Q/E179Q

Plasmid

#223819

Purpose

Bacterial expression of SynPspA R44K/E126Q/E179Q with N-terminal 6xHis-Tag and 3C cleavage site

Depositor

Carsten Sachse

Article

Junglas et al Nat Struct Mol Biol. 2025 Jan;32(1):23-3

Insert

SynPspA

Tags

6xHis

Expression

Bacterial

Mutation

R44K, E126Q, E179Q

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYTRW10K_0x5

Plasmid

#177278

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Type

Empty backbone

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pN3-sTRAIL

Plasmid

#154246

Purpose

Plasmid compatible with non-viral delivery for secretion of trimeric human TRAIL

Depositor

Jordan Green

Article

Vaughan et al Mol Ther Oncolytics. 2021 Apr 16;21:377-

Insert

Secretable Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand

Expression

Mammalian

Promoter

CMV Promoter

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pHS1625

Plasmid

#239834

Purpose

OrufIscB-REC E. coli expression for recombinant purification

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

Insert

OrufIscB-REC

Tags

14xHis-TwinStrep-bdSUMO

Expression

Bacterial

Mutation

Insertion of REC domain from NbaCas9-1

Promoter

T7

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pHS1505

Plasmid

#239830

Purpose

OrufIscB-REC mammalian expression

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

Insert

OrufIscB-REC

Tags

Nucleoplasmin NLS-3xHA and SV40 NLS

Expression

Mammalian

Mutation

Insertion of REC domain from NbaCas9-1

Promoter

CMV

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac1-ScVip1-186-1107

Plasmid

#238316

Purpose

ScVip1-186-1107 protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-186-1107

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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1pBB3-ScVip1-536-1107-C793A

Plasmid

#238324

Purpose

1ScVip1-536-1107-C793A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-C793A

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-186-522

Plasmid

#238311

Purpose

ScVip1-186-522 protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-186-522

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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