Addgene

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Showing: 2801 - 2850 of 49422 results

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pOG2002

Plasmid

#49514

Purpose

TALEN backbone for expression of TALE(deltaRDV)-FokI AS in C. elegans

Depositor

Guangshuo Ou

Article

Cheng et al Nat Biotechnol. 2013 Oct;31(10):934-7. d

Type

Empty backbone

Use

Tags

3xFLAG, FokI-AS, and SV40 NLS

Expression

Worm

Mutation

Promoter

hsp-16.2

Available since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pGEL854

Plasmid

#214254

Purpose

Expression eTd-CBE in rice cells

Depositor

Yong Zhang

Article

Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB11785

Plasmid

#212699

Purpose

gRNA targeting to the intergradtion site E4

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site E4

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB10769

Plasmid

#212694

Purpose

gRNA targeting to the intergradtion site E2

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site E2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB12719

Plasmid

#212702

Purpose

gRNA targeting to the intergradtion site F3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site F3

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB11787

Plasmid

#212695

Purpose

gRNA targeting to the intergradtion site E2

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site E2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB6637

Plasmid

#212696

Purpose

gRNA targeting to the intergradtion site E3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site E3

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB8860

Plasmid

#212697

Purpose

gRNA targeting to the intergradtion site E3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site E3

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB8856

Plasmid

#212701

Purpose

gRNA targeting to the intergradtion site C3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site C3

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB8866

Plasmid

#212703

Purpose

gRNA targeting to the intergradtion site F3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site F3

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB6630

Plasmid

#212700

Purpose

gRNA targeting to the intergradtion site C3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site C3

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB6638

Plasmid

#212698

Purpose

gRNA targeting to the intergradtion site E4

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site E4

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-KinK

Plasmid

#139596

Purpose

expresses KinK in E. coli

Depositor

Emily Balskus

Article

Wang et al Nat Commun. 2018 Sep 11;9(1):3687. doi:

Insert

kinK

Use

Tags

hexahistidine

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-KinL

Plasmid

#139595

Purpose

expresses KinL in E. coli

Depositor

Emily Balskus

Article

Wang et al Nat Commun. 2018 Sep 11;9(1):3687. doi:

Insert

kinL

Use

Tags

hexahistidine

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-KinM

Plasmid

#139594

Purpose

expresses KinM in E. coli

Depositor

Emily Balskus

Article

Wang et al Nat Commun. 2018 Sep 11;9(1):3687. doi:

Insert

kinM

Use

Tags

hexahistidine

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-KinN

Plasmid

#139593

Purpose

expresses KinN in E. coli

Depositor

Emily Balskus

Article

Wang et al Nat Commun. 2018 Sep 11;9(1):3687. doi:

Insert

kinN

Use

Tags

hexahistidine

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCasPA

Plasmid

#113347

Purpose

Bacterial expression of Cas9 nuclease and λ-Red system in Pseudomonas aeruginosa

Depositor

Quanjiang Ji

Article

Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pACRISPR

Plasmid

#113348

Purpose

A sgRNA expression plasmid for genome editing in Pseudomonas aeruginosa

Depositor

Quanjiang Ji

Article

Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF6a-CD28-PafA

Plasmid

#113400

Purpose

Expresses CD28-PafA in mammalian cell

Depositor

Min Zhuang

Article

Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

Insert

CD28-PafA

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

EF-1α

Available since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Frb-EGFP-HOTag6

Plasmid

#106919

Purpose

Express Frb-EGFP-HOTag6 fusion in mammalian cells.

Depositor

Xiaokun Shu

Article

Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

Insert

Frb-EGFP-HOTag6

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-FKBP-EGFP-HOTag3

Plasmid

#106924

Purpose

Expresses FKBP-EGFP-HOTag3 in mammalian cells

Depositor

Xiaokun Shu

Article

Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

Insert

FKBP-EGFP-HOTag3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
px330-mcherry

Plasmid

#98750

Purpose

Cas9 from S.pyogenes with CMV-mcherry cassette, and cloning backbone for sgRNA

Depositor

Jinsong Li

Article

Wu et al Cell Stem Cell. 2013 Dec 5;13(6):659-62.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-AsCas12f1

Plasmid

#171614

Purpose

AsCas12f1-based Human cell genome editing

Depositor

Quanjiang Ji

Article

Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

Insert

AsCas12f1 and sgRNA_v1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEVOL-pylT-AznLRS

Plasmid

#172482

Purpose

For E.Coli expression, 306L309L348I384F411W

Depositor

Wenshe Liu

Article

Wang et al Angew Chem Int Ed Engl. 2017 Feb 1;56(6)

Insert

AznLRS and pylTcua

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p15a-AsCas12f-blaZ

Plasmid

#171609

Purpose

AsCas12f1-based E. coli genome editing

Depositor

Quanjiang Ji

Article

Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

Insert

AsCas12f1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-PKA-SPARK

Plasmid

#106920

Purpose

Express PKA-SPARK, an activity reporter of protein kinase A in mammalian cells.

Depositor

Xiaokun Shu

Article

Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

Insert

PKA-SPARK

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-ERK-SPARK

Plasmid

#106921

Purpose

Express ERK-SPARK, an activity reporter of ERK in mammalian cells.

Depositor

Xiaokun Shu

Article

Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

Insert

ERK-SPARK

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-NHERF1

Plasmid

#31650

Depositor

Edward Weinman

Article

Weinman et al Biochemistry. 2003 Nov 4. 42(43):12662-8

Insert

solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1 Rabbit)

Use

Tags

His & S tag

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Feb. 14, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pMCP-RecT

Plasmid

#183827

Purpose

pLenti-EF1A-MCP-EXTEN-RecT-NLS

Depositor

Le Cong

Article

Wang et al Nat Cell Biol. 2022 Feb;24(2):268-278. d

Insert

MCP-RecT_NLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF6a-BCCP-PupE

Plasmid

#113399

Purpose

Expresses BCCP-PupE in mammalian cell

Depositor

Min Zhuang

Article

Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

Insert

BCCP-PupE

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF-1α

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pV1F(Spec)

Plasmid

#205086

Purpose

Prokaryotic expressing of AtVIP1 fused with a sequence encoding a T4SS translocation tag in A. tumefaciens.

Depositor

Suomeng Dong

Article

Wang et al PLoS Pathog. 2023 Apr 21;19(4):e1011346.

Insert

AtVIP1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pUASattB-ERK-SPARK

Plasmid

#106923

Purpose

Express ERK-SPARK, an activity reporter of ERK in Drosophila

Depositor

Xiaokun Shu

Article

Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

Insert

ERK-SPARK

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX6p-1-BCCP-PupE

Plasmid

#113403

Purpose

Expresses BCCP-PupE in bacteria

Depositor

Min Zhuang

Article

Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

Insert

BCCP-PupE

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
miniCMV-iRFP670-tDeg

Plasmid

#185403

Purpose

iRFP670-tDeg fluorogenic protein

Depositor

Jian-Hui Jiang

Article

Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

Insert

iRFP670-tDeg

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p15a-AsCas12f-apmR

Plasmid

#171610

Purpose

AsCas12f1-based K. pneumoniae genome editing

Depositor

Quanjiang Ji

Article

Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

Insert

AsCas12f1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCpf1

Plasmid

#122185

Purpose

Bacterial genome editing plasmid with kanamycin resistance marker

Depositor

Cheng-Cai Zhang

Article

Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF6a-FKBP-CD28

Plasmid

#113401

Purpose

Expresses FKBP-CD28 in mammalian cell

Depositor

Min Zhuang

Article

Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

Insert

FKBP-CD28

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF-1α

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCpf1-sp

Plasmid

#122186

Purpose

Bacterial genome editing plasmid with spectinomycin resistance marker

Depositor

Cheng-Cai Zhang

Article

Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSBY1_FnCpf1cg

Plasmid

#104622

Purpose

CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn CPf1

Depositor

Sheng Yang

Article

Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

Insert

FnCpf1

Use

CRISPR

Tags

Expression

Bacterial

Mutation

codon-optimized C.glutamicum

Promoter

hsp60

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX6p-1-FRB-PafA

Plasmid

#113404

Purpose

Expresses FRB-PafA in bacteria

Depositor

Min Zhuang

Article

Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

Insert

FRB-PafA

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSBY3_ligD

Plasmid

#104624

Purpose

CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD

Depositor

Sheng Yang

Article

Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

Insert

FnCpf1

Use

CRISPR

Tags

Expression

Bacterial

Mutation

FnCpf1 codon-optimized C.glutamicum

Promoter

hsp60

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
PET-3a-chpBK

Plasmid

#155201

Purpose

To express recombinant ChpBK protein

Depositor

Guanzheng Luo

Article

Zhang et al Sci Adv. 2019 Jul 3;5(7):eaax0250. doi:

Inserts

chpS-chpBK-6×His-Tag
GyrA-CBD-10xHis-Tag

Use

Tags

10xHis and 6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pUASattB-PKA-SPARK

Plasmid

#106922

Purpose

Express PKA-SPARK, an activity reporter of protein kinase A in Drosophila

Depositor

Xiaokun Shu

Article

Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

Insert

PKA-SPARK

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-SUMO-AsCas12f-2NLS

Plasmid

#171613

Purpose

NLS-AsCas12f1-NLS heterologous expression plasmid for RNP

Depositor

Quanjiang Ji

Article

Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

Insert

NLS-AsCas12f1-NLS

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-EF1aL-nlsGFP-W

Plasmid

#126688

Purpose

Constitutive, ubiquitous, nuclear-localized GFP expression.

Depositor

Darrell Kotton

Article

Tang et al Nat. Phys. 18, 1371–1378 (2022).

Insert

nlsGFP

Use

Lentiviral

Tags

6xHIS and NLS

Expression

Mutation

Promoter

EF1aL

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
gRNAU6

Plasmid

#135963

Purpose

Contains tracrRNA-hU6 sequence for double sgRNA cloning

Depositor

Yongming Wang

Article

Xie et al Sci Rep. 2017 May 24;7(1):2320. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSBY2_ligD(FnCpf1)

Plasmid

#104623

Purpose

CRISPR system used to genome editing in Mycobacterium smegmatis, expresses crRNA to ligD

Depositor

Sheng Yang

Article

Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

Insert

crRNA to ligD

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Pj23119

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
miniCMV-tdTomado-tDeg

Plasmid

#185402

Purpose

tdTomado-tDeg fluorogenic protein

Depositor

Jian-Hui Jiang

Article

Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

Insert

tdTomado-tDeg

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEL636

Plasmid

#189830

Purpose

The T-DNA backbone of Cas12j2 for transcriptional activation in rice

Depositor

Yong Zhang

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

pZmUbi1

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX6p-1-PafA-XIAP

Plasmid

#113402

Purpose

Expresses PafA-XIAP in bacteria

Depositor

Min Zhuang

Article

Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

Insert

PafA-XIAP

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

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