We narrowed to 11,731 results for: Emb;
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Plasmid#108713PurposeEF1-CBR2opt-T2A-copGFP (construct used for lentiviral transduction)DepositorInsertCBR2opt-copGFP fusion
UseLentiviralTagsCBR2opt fused to copGFPMutationPromega used directed evolution to create CBR (Cl…Promoterelongation factor-1Available SinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
hSERT-N1-pEYFP
Plasmid#70105Purposemammalian expression of hSERT with C-terminal YFP fusionDepositorAvailable SinceNov. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CHRNA7-mCherry
Plasmid#62632Purposemammalian expression of human Alpha7 (CHRNA7) with mCherry fused into M3-M4 loopDepositorInserthAlpha7 (CHRNA7 Human)
ExpressionMammalianMutationmCherry inserted into M3-M4 loopPromoterCMVAvailable SinceMarch 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Tubulin K40Q
Plasmid#105302PurposeTo study effects of acetyl-mimetic (hyper-acetylated) tubulinDepositorAvailable SinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCZGY2849
Plasmid#74112PurposeEnhanced mutant version of membrane targeted PH-miniSOG (Q103L) entry clone for more efficient ablation of cells when expressed under desired promoter.DepositorInsertminiSOG(Q103L)
UseGateway topo cloningTagsPHAvailable SinceApril 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMU-GAr
Plasmid#61172PurposeFluorescent reporter for Golgi apparatus GmMAN49-mCherry expressed under AtUBQ10 promoterDepositorInsertGmMAN49-mCherry
ExpressionPlantPromoterAtUBQ10Available SinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
Dead Streptavidin-SpyCatcher
Plasmid#59547PurposepET21-DCatch encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyCatcher, for generation of chimeric SpyAvidin tetramersDepositorInsertDead-dloop-Streptavidin-SpyCatcher
TagsN-terminally shortened SpyCatcherExpressionBacterialMutationPolyAsp in L3/4 loop for ion-exchangeAvailable SinceOct. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pCMV-eGFP-NMHC-IIAdeltaIQ2
Plasmid#35690DepositorInsertMHC IIA (MYH9 Human)
TagsEGFP and GFPGenotRWExpressionMammalianMutationThe coding segment for aa 804-834 (corresponding …PromoterCMVAvailable SinceApril 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-hATG2A
Plasmid#114462PurposeExpresses human ATG2A with GFP in mammalian cellsDepositorInserthuman ATG2A (ATG2A Human)
UseRetroviralTagsGFPExpressionMammalianMutationP656R (please see depositors comment below)PromoterCMVAvailable SinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p1-GST-Rnf31
Plasmid#63134PurposeExpression of human RNF31/HOIP codon optimized for E. coli and insect cellsDepositorInsertRnf31 (RNF31 Human, Synthetic)
TagsGSTExpressionBacterialMutationSynthetic based on human protein sequencePromotertacAvailable SinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGIP*35
Plasmid#26776PurposeEnables the creation of a chromosomally integrated mutant EGFP target sequence by lentiviral transduction to assess the efficiency of HR-mediated gene targetingDepositorInsertGFP*35
UseLentiviralExpressionMammalianAvailable SinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TRE3G-BCCP-PupE-IRES-BFP
Plasmid#113405PurposeExpress inducible BCCP-PupE in mammalian cellDepositorInsertBCCP-PupE-IRES-BFP
UseLentiviralExpressionMammalianAvailable SinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcU6_3 sgRNA
Plasmid#92359PurposeChicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter.DepositorInsertchick U6.3 promoter and gRNA cloning cassette
UseCRISPRExpressionMammalianPromoterchick U6.3Available SinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGB 35S:Renilla:Tnos (GB0109)
Plasmid#68215PurposeTranscriptional unit for Renilla Luciferase Protein plant expression driven by the 35S promoterDepositorInsertRenilla
UseLuciferase and Synthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable SinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
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GFPZFN1.4-B2H
Plasmid#26791DepositorInsertGFP-ZFN1
ExpressionMammalianAvailable SinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
Capping Protein
Plasmid#89950PurposeF-actin-capping proteins bind in a Ca2+-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends.DepositorInsertsTags6*HisExpressionBacterialMutationV222IAvailable SinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSJ1321 (pRS315-NOP1pr-GFP11-mCherry-PUS1)
Plasmid#86413Purposenuclear split GFP11 reporter with mCherryDepositorInsertNop1pr-GFP11-mCherry-PUS1
TagsGFP11 and mCherryExpressionYeastPromoterNOP1Available SinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only