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Plasmid#161784PurposeGateway destination vector with bidirectional CMV-PGK promoter for modest expression of gene of interest and TagBFP.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterBidirectional minhCMV and hPGKAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only
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p944-SWITCH-OVER insert: EF1a-Puro
Plasmid#217891Purposeinsert vector for Switch-OVER: Single loxP-STOP-EF1a-Puro-mU6DepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAL
Plasmid#161783PurposeGateway destination vector with bidirectional CMV-PGK promoter for modest expression of gene of interest and GFP.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterBidirectional minhCMV and hPGKAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV GG hUbV-EGFP
Plasmid#216161PurposeContains a Golden-Gate cloning cassette to express up to four gRNA and EGFPDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSB700 HL1gR4
Plasmid#124450PurposeExpresses gRNA HL1 gR4 in the pSB700 backboneDepositorInsertHL1 gR4
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pScalps eSpCas9_Zsgreen gRNA scaffold
Plasmid#188449PurposeExpression of eSpCas9 tagged with ZsgreenDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterEF1-alphaAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCsy-crRNA-EV
Plasmid#153944PurposeFor cloning and expression of Type I-F P. aeruginosa (PaeCascade) crRNA in mammalian cellsDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPKm-145
Plasmid#90505PurposeEmpty plasmid, pSIN-EF1-alpha-IRES-puroDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSECB
Plasmid#106305PurposeExpress spCas9 with sgRNA, blast resistanceDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCVL TAL(Nd154C63) SFFV Age-Xho/Xba-Sal linkers
Plasmid#50421PurposepTAL plasmid for cloning of megaTALs or other TAL-fusions and expression in mammalian cells. RVDs are cloned between Age-Xho sites and meganuclease/fusion is cloned between Xba-Sal sites.DepositorTypeEmpty backboneUseLentiviral and TALENTagsExpressionMammalianMutationPromoterSFFVAvailable sinceMay 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCVL TAL(Nd154C63) SFFV Xba-Sal linker
Plasmid#50420PurposepTAL plasmid compatible with the Golden Gate TALEN cloning system. For cloning of megaTALs or other TAL-fusions and expression in mammalian cells. Meganuclease/fusion is cloned between Xba-Sal sites.DepositorTypeEmpty backboneUseLentiviral and TALENTagsExpressionMammalianMutationPromoterSFFVAvailable sinceMay 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHAGE Flag HA ADAR2
Plasmid#237856PurposeLentiviral expression of ADAR2DepositorInsertADAR2 (ADARB1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
p931-SWITCH-OVER insert: EF1a-Blasti
Plasmid#217890Purposeinsert vector for Switch-OVER: Single loxP-STOP-EF1a-Blasti-mU6DepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-MCS-EnR_C-term
Plasmid#136885PurposeDoxycycline-inducible vector backbone containing a multiple cloning site (MCS), the engrailed (EnR) repressor domain and a STOP codonDepositorInsertengrailed repressor domain (en Fly)
UseLentiviralTagsNoneExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO5.U6.DR130(CasRX)_Empty.EFS.tRFP657
Plasmid#212961PurposeLentiviral plasmid for the cloning of mature gRNAs of the RfxCas13d (CasRx) CRISPR system. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.DepositorInsertDR1_CasRx_backbone
UseCRISPR and LentiviralTagsTagRFP657ExpressionMammalianMutationPromoterU6Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
lenti-sgRNA blast
Plasmid#104993PurposeThis 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and blasticidin S resistance from an EF-1a promoter.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
lenti-sgRNA puro
Plasmid#104990PurposeThis 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and puromycin resistance from an EF-1a promoter.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
lenti-sgRNA hygro
Plasmid#104991PurposeThis 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and hygromycin resistance from an EF-1a promoter.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
lenti-sgRNA neo
Plasmid#104992PurposeThis 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and G418 (neomycin) resistance from an EF-1a promoter.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-neo-YAP
Plasmid#166468PurposePlasmid coding for YAP and neomycin resistance in a lentiviral backbone.DepositorInsertYAP, Neo (YAP1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only