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We narrowed to 67,652 results for: plasmid

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Showing: 1121 - 1140 of 67652 results

  1. pSL1521 (pSPIN, pSC101* backbone)

    Plasmid
    #160729
    Purpose
    Single-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.
    Depositor
    Samuel H. Sternberg
    Publication
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Expression
    Bacterial
    Promoter
    J23119
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    160729-plasmid-map-sequence-id-322926

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  2. pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (AAV1)

    Viral prep
    #162379-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (#162379). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8f-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of the ultrafast calcium sensor, GCaMP8f. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Publication
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    Syn
    Available since
    May 18, 2021
    Availability
    Academic Institutions and Nonprofits only

  3. pOTTC1596 - pAAV SYN1 HA-hM3D(Gq) (AAV5)

    Viral prep
    #121539-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pOTTC1596 - pAAV SYN1 HA-hM3D(Gq) (#121539). In addition to the viral particles, you will also receive purified pOTTC1596 - pAAV SYN1 HA-hM3D(Gq) plasmid DNA. Synapsin-driven, HA-tagged hM3D(Gq) for neuronal activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Christopher Richie
    Publication
    Galvan et al Eur J Neurosci. 2019 May 7. doi: 10.1111
    Promoter
    Syn
    Tags
    HA
    Available since
    Feb. 21, 2019
    Availability
    Academic Institutions and Nonprofits only

  4. pAAV-CaMKIIa-eNpHR 3.0-EYFP (AAV9)

    Viral prep
    #26971-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-CaMKIIa-eNpHR 3.0-EYFP (#26971). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-eNpHR 3.0-EYFP plasmid DNA. CaMKIIa-driven eNpHR 3.0-EYFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    CaMKII
    Tags
    EYFP
    Available since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only

  5. pEGFP-C1-FLAG

    Plasmid
    #46956
    Purpose
    Plasmid for cloning and expression of GFP-FLAG tagged proteins (N-terminal tag). Confers resistance to G418.
    Depositor
    Steve Jackson
    Publication
    Britton et al J Cell Biol. 2013 Jul 29.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FLAG tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    46956-plasmid-map-sequence-id-352459

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  12. hACE2

    Plasmid
    #1786
    Purpose
    Mammalian expression of human ACE2
    Depositor
    Hyeryun Choe
    Publication
    Li et al Nature. 2003 Nov 27. 426(6965):450-4.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    C9 (GTETSQVAPA)
    Expression
    Mammalian
    Available since
    Feb. 14, 2006
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1786-plasmid-map-sequence-id-275247

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  13. pAAV-CAG-GFP (AAV1 trial size)

    Viral prep
    #37825-AAV1.T
    Purpose
    Ready-to-use AAV1 trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Promoter
    CAG
    Tags
    GFP
    Available since
    May 29, 2019
    Availability
    Academic Institutions and Nonprofits only

  15. BPK764

    Plasmid
    #65767
    Purpose
    Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65767-plasmid-map-sequence-id-471631

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  16. pAAV-hSyn-EGFP (AAV9 trial size)

    Viral prep
    #50465-AAV9.T
    Purpose
    Ready-to-use AAV9 trial size particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    EGFP
    Available since
    May 29, 2019
    Availability
    Academic Institutions and Nonprofits only

  18. pENN.AAV.CMVs.Pl.Cre.rBG

    Plasmid
    #105537
    Purpose
    AAV expression of Cre from CMV promoter
    Depositor
    James M. Wilson
    Has service
    AAV1, AAV2, AAV5, AAV8, AAV9, and AAV rh10
    Insert
    Cre
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    105537-plasmid-map-sequence-id-202428

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  19. YopH (residues 164-468)

    Plasmid
    #79749
    Purpose
    This plasmid encodes the active catalytic domain (amino acid residues 164-468) of YopH. Inteded for co-expression with human Tyr kinase plasmids to enhance bacterial kinase expression.
    Depositor
    John Chodera, Nicholas Levinson, Markus Seeliger
    Publication
    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/
    Insert
    YopH (residues 164-468)
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    79749-plasmid-map-sequence-id-443904

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  20. pPP441

    Plasmid
    #158801
    Purpose
    Plasmid containing Homo sapiens codon optimized CasΦ-2 and a SapI-GG stuffer spacer for editing in human cells.
    Depositor
    Jennifer Doudna
    Publication
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CasPhi-2
    Tags
    2xSV40NLS-2xFLAG
    Expression
    Mammalian
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    158801-plasmid-map-sequence-id-309574

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Showing: 1121 - 1140 of 67652 results