We narrowed to 6,025 results for: plasmid dna
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Plasmid#113458PurposeNanoLuc-BCL2L1 expression vector (positive control together with pcDNA3.1 PA-mCit-BAD)DepositorInsertBCL2 like 1 (BCL2L1 Human)
UseLuciferaseTagscmyc-NanoLucExpressionMammalianMutationPromoterCMVAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MF3958 Heavy chain-SpyTag003
Plasmid#216303PurposeExpresses anti-HER2 MF3958 Heavy chain-SpyTag003 in mammalian cells. To be paired with MF3958 light chain to form the anti-HER2 MF3958 FabDepositorInsertMF3958 Heavy chain-SpyTag003
UseAffinity Reagent/ AntibodyTagsHis6 tag, Signal peptide, and SpyTag003ExpressionMammalianMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-39S Heavy chain-SpyTag003
Plasmid#216296PurposeExpresses anti-HER2 39S Heavy chain-SpyTag003 in mammalian cells. To be paired with 39S light chain to form the anti-HER2 39S FabDepositorInsert39S Heavy chain-SpyTag003
UseAffinity Reagent/ AntibodyTagsHis6 tag, Signal peptide, and SpyTag003ExpressionMammalianMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgL-FLAG (TB40/E)
Plasmid#136449PurposeMammalian expression plasmid for HCMV glycoprotein L (TB40/E strain) with FLAG tagDepositorInsertglycoprotein L (UL115 Human betaherpesvirus 5)
UseTagsShort SGGSG linker and FLAG tagExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgH-8his (Merlin)
Plasmid#136447PurposeMammalian expression plasmid for HCMV glycoprotein H (Merlin strain) extracellular domain with 8his purification tagDepositorInsertglycoprotein H (UL75 Human betaherpesvirus 5)
UseTagsShort GGSG linker and 8his tagExpressionMammalianMutationExtracellular domain (a.a. 1-716). Codon-optimize…PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-Tet3 CD Mut
Plasmid#72222PurposeExpress flag-tagged mouse Tet3 catalytic domain - catalytic dead mutant.DepositorInsertTen-Eleven Translocation 3
UseTagsflagExpressionMammalianMutationThe plasmid expresses TET3 catalytic domain (cata…PromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA_SZ1-RGG-GFP-RGG
Plasmid#124932PurposeExpression of SZ1-RGG-GFP-RGGDepositorInsertSYNZIP1-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag
Plasmid#154970PurposeExpresses N-terminally GFP-tagged EBA175 (residues 1284-1462) from pcDNA3.1. Please note: Plasmid contains a Stop codon prior to the C-terminal Flag tag.DepositorInsertPfEBA175
UseTagsGFP and signal peptide (residues 1 to 32) from TG…ExpressionMammalianMutationinsert codes for EBA175 residues 1284 to 1462PromoterCMVAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gα15-244-NLuc
Plasmid#207154PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA15 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNA15 (GNA15 Human)
UseTagsExpressionMammalianMutationNLuc inserted at position E(244)NPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgO-8his (Merlin)
Plasmid#136454PurposeMammalian expression plasmid for HCMV glycoprotein O (Merlin strain) with an 8his tagDepositorInsertglycoprotein O (UL74 Human betaherpesvirus 5)
UseTagsLinker and 8his tag (GGSGHHHHHHHH, includes BamHI…ExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gαq-124-NLuc
Plasmid#207153PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAQ tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAQ (GNAQ Human)
UseTagsExpressionMammalianMutationNLuc inserted at position F(124)EPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/HP + AUG-nLuc-3XFLAG
Plasmid#127322PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with hairpin in 5' UTRDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-Lin28A-mCCHC
Plasmid#51372DepositorInsertLin28A (LIN28A Human)
UseTagsFLAGExpressionMammalianMutationCCHC domain mutant (H147A, H169A)PromoterCMVAvailable sinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 mir-1-1 reporter mir30-5
Plasmid#46692DepositorInsertmir30-5 (MIR30A Synthetic, Human)
UseRNAiTagsV5 and hsa-mir-1-1ExpressionMammalianMutationsee sequence and publicationPromoterCMVAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-mNeonGreen-NUT
Plasmid#204693PurposeThis plasmid allows for inducible expression of full-length NUT tagged with mNeonGreen, in mammalian cells.DepositorInsertNUT (NUTM1 Human)
UseTagsmNeonGreenExpressionMammalianMutationn/aPromoterCMVAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/HP + GUG-nLuc-3XFLAG
Plasmid#127323PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with hairpin in 5' UTRDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 mir-1-1 reporter mir193b-3
Plasmid#46733DepositorInsertmir193b-3 (MIR193B Synthetic, Human)
UseRNAiTagsV5 and hsa-mir-1-1ExpressionMammalianMutationsee sequence and publicationPromoterCMVAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 mir-1-1 reporter mir193b-2
Plasmid#46732DepositorInsertmir193b-2 (MIR193B Synthetic, Human)
UseRNAiTagsV5 and hsa-mir-1-1ExpressionMammalianMutationsee sequence and publicationPromoterCMVAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 mir-1-1 reporter mir193b-1
Plasmid#46731DepositorInsertmir193b-1 (MIR193B Synthetic, Human)
UseRNAiTagsV5 and hsa-mir-1-1ExpressionMammalianMutationsee sequence and publicationPromoterCMVAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 mir-1-1 reporter mir30-7
Plasmid#46694DepositorInsertmir30-7 (MIR30A Synthetic, Human)
UseRNAiTagsV5 and hsa-mir-1-1ExpressionMammalianMutationsee sequence and publicationPromoterCMVAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only