We narrowed to 1,221 results for: poli
-
-
pcDNA-dCas9-p300 Core
Plasmid#61357Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterCMVAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (D1399Y)
Plasmid#61358Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; D1399Y mutation i…PromoterCMVAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 S HexaPro
Plasmid#154754PurposeMammalian expression vector for expression of the SARS-CoV-2 spike HexaPro variantDepositorInsertSpike (HexaPro variant) (S Severe acute respiratory syndrome coronavirus 2)
UseTags2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …ExpressionMammalianMutationEctodomain only (AAs 1-1208); 682-685 (furin site…PromoterAvailable sinceJuly 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-Sport6-CD63-pHluorin
Plasmid#130901PurposeExpression of CD63-pHluorin for visualization of multivesicular body-plasma membrane fusion.DepositorInsertCD63-pHluorin (CD63 Aequorea victoria, Human)
UseTagspHluorinExpressionMammalianMutationpHluorin inserted between Gln-36 and Leu-37 in ex…PromoterCMVAvailable sinceOct. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Sport6-CD63-pHuji
Plasmid#130902PurposeExpression of CD63-pHuji for visualization of multivesicular body-plasma membrane fusion.DepositorInsertCD63-pHuji (CD63 Human, synthetic)
UseTagspHujiExpressionMammalianMutationpHuji inserted between Gln-36 and Leu-37 in extra…PromoterCMVAvailable sinceOct. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Sport6-CD81-pHuji
Plasmid#130904PurposeExpression of CD81-pHuji for visualization of multivesicular body-plasma membrane fusion.DepositorInsertCD81-pHuji (CD81 Synthetic, Human)
UseTagspHujiExpressionMammalianMutationpHuji inserted between Leu-49 and Gly-50 in extra…PromoterCMVAvailable sinceOct. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Sport6-CD9-pHluorin
Plasmid#130905PurposeExpression of CD9-pHluorin for visualization of multivesicular body-plasma membrane fusion.DepositorInsertCD9-pHluorin (CD9 Aequorea victoria, Human)
UseTagspHluorinExpressionMammalianMutationphluorin inserted between Asn-50 and Asn-51 in ex…PromoterCMVAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Sport6-CD81-pHluorin
Plasmid#130903PurposeExpression of CD81-pHluorin for visualization of multivesicular body-plasma membrane fusion.DepositorInsertCD81-pHluorin (CD81 aequorea victoria, Human)
UseTagspHluorinExpressionMammalianMutationpHluorin inserted between Leu-49 and Gly-50 in ex…PromoterCMVAvailable sinceOct. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Sport6-CD9-pHuji
Plasmid#130906PurposeExpression of CD9-pHuji for visualization of multivesicular body-plasma membrane fusion.DepositorInsertCD9-pHuji (CD9 Synthetic, Human)
UseTagspHujiExpressionMammalianMutationpHuji inserted between Asn50 and Asn51 in extrace…PromoterCMVAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (Y1467F)
Plasmid#61362Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inavtivating mutation Y1467F) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; Y1467F mutation i…PromoterCMVAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHIS-MDH1-HIS
Plasmid#184558PurposeBacterial expression of human MDH1 with HIS TagDepositorInsertMalate dehydrogenase 1 (MDH1 Human)
UseTags6xHISExpressionBacterialMutationPromoterAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (1645/1646 RR/EE)
Plasmid#61359Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation 1645/1646 RR/EE) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; 1645/1646 RR/EE m…PromoterCMVAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (1396/1397 SY/WW)
Plasmid#61363Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation 1396/1397 SY/WW) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; 1396/1397 SY/WW m…PromoterCMVAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (C1204R)
Plasmid#61361Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation C1204R) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; C1204R mutation i…PromoterCMVAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)
Plasmid#61364Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; H1415A/E1423A/Y14…PromoterCMVAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLPC-puromycin-binary-MDH1-3xFLAG-ME1- HA
Plasmid#184549PurposeRetroviral vector to co-express human MDH1 with 3xFLAG tag and human ME1 with HA tagDepositorUseRetroviralTags3xFLAG and HA tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUltra-MDH1-ME1
Plasmid#184465PurposeLentiviral vector for tri-cistronic expression of EGFP, MDH1 and ME1 (seperated by P2A and T2A)DepositorUseLentiviralTagsfused to T2AExpressionMammalianMutationdeletion of stop codonPromoterUbCAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_ spike_del19
Plasmid#155297PurposeExpression of spike with C-term deletion of 19 aa, used to generate high efficiency SARS-CoV-2-pseudotyped lentiviral particlesDepositorInsertSARS-Cov2 spike_deleted (S SARS-Cov2)
UseGeneration of sars-cov-2-pseudotyped lentiviral p…TagsExpressionMutationDeletion in C-term 19 aa of spikePromoterCMVAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLKO.1-blast shGFP
Plasmid#110419PurposeshGFP control, silence GFP gene, blasticidin selection.DepositorInsertGFP
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6 (RNA PolIII)Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only
