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pCMV-Tag3B-CCre

Plasmid

#106369

Purpose

C-terminal part of the split-Cre System

Depositor

Johannes Hirrlinger

Article

Hirrlinger et al PLoS One. 2009;4(1):e4286. doi: 10.1371/

Insert

CCre

Use

Cre/Lox

Tags

c-myc

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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aCKAR

Plasmid

#124427

Purpose

FRET-based reporter for monitoring aPKC activity in cells

Depositor

Alexandra Newton

Article

Kajimoto et al Sci Signal. 2019 Jan 1;12(562). pii: 12/

Insert

aCKAR

Tags

mCFP and mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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mTurquoise2-LaminB

Plasmid

#98830

Purpose

In vivo visualization of the nuclear envelope (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

LaminB

Tags

mTurquoise2

Expression

Mammalian

Mutation

*(see below)

Promoter

CMV

Available Since

Nov. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pBMN DHFR(DD)-YFP

Plasmid

#29325

Depositor

Thomas Wandless

Article

Iwamoto et al Chem Biol. 2010 Sep 24. 17(9):981-8.

Insert

DHFR(DD)-YFP

Use

Retroviral

Tags

HA and YFP

Mutation

Change Arg 12 to Tyr (R12Y),Gly 67 to Ser (G67S) …

Available Since

July 1, 2011

Availability

Academic Institutions and Nonprofits only

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pH6HTC_ENO1-Halo

Plasmid

#175330

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

ENO1 (ENO1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pET15bpATnp

Plasmid

#121137

Purpose

This vector encode a His6-tagged proteinA-Tnp(hyperactive form) fusion protein.

Depositor

Keji Zhao

Article

Carter et al Nat Commun. 2019 Aug 20;10(1):3747. doi:

Insert

Protein A-Tnp

Tags

his

Expression

Bacterial

Promoter

T7

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pDA077

Plasmid

#131388

Purpose

L1RP expression

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

L1RP

Expression

Mammalian

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pDAS12395_PEAR-GFP_2.0-preedited

Plasmid

#177185

Purpose

PEAR-GFP3 control plasmid with split EGFP and intact 5' splice site

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Insert

EGFP split with an intron between amino acids 95-96

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pBbA5c-MevT(CO)-T1-MBIS(CO, ispA)

Plasmid

#35152

Depositor

Jay Keasling, Taek Soon Lee

Article

Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

Insert

AtoB(co)-HMGS(co)-HMGR(co)-pTrc-MK(co)-PMK(co)-PMD-Idi-IspA

Use

Synthetic Biology

Expression

Bacterial

Mutation

R364S in Mevalonate Kinase

Promoter

LacUV5, pTrc

Available Since

May 16, 2012

Availability

Academic Institutions and Nonprofits only

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p-G4PID-HA

Plasmid

#209639

Purpose

Expression G4PID-HA in mammalian cells

Depositor

Xiang Zhou

Article

Lu et al Nucleic Acids Res. 2024 Mar 7:gkae126. d

Insert

G4PID (DHX36 Human)

Expression

Mammalian

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-cMSP26

Plasmid

#199769

Purpose

Expresses split-intein fused MSP26 for circularization during expression (internal His-Tag)

Depositor

Franz Hagn

Article

Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

Insert

cMSP26

Tags

His6-Tag

Expression

Bacterial

Mutation

internal His-Tag

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-cMSPdH4-6

Plasmid

#199766

Purpose

Expresses split-intein fused MSPdH4-6 for circularization during expression (internal His-Tag)

Depositor

Franz Hagn

Article

Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

Insert

cMSPdH46

Tags

His6-Tag

Expression

Bacterial

Mutation

internal His-Tag

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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Tol2-UAS-Dendra2

Plasmid

#97145

Purpose

For Gal4/UAS expression system

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

Dendra2

Use

Zebrafish

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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p-G4PID

Plasmid

#209638

Purpose

Expression G4PID in mammalian cells

Depositor

Xiang Zhou

Article

Lu et al Nucleic Acids Res. 2024 Mar 7:gkae126. d

Insert

G4PID (DHX36 Human)

Expression

Mammalian

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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p201N Cas9

Plasmid

#59175

Purpose

Cas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

Depositor

Wayne Parrott

Article

Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

Inserts

Cas9
nptII

Use

CRISPR

Promoter

2x35S and StUbi-3P

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pACS141

Plasmid

#204597

Purpose

Histone H3.3-V5

Depositor

Tom Misteli

Article

Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

Insert

human histone H3.3 (H3-3A Human)

Tags

human histone H3.3 - V5

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-IvfChr-citrine

Plasmid

#221614

Purpose

A recombinant AAV2 plasmid encoding vfChrimson-citrine with trafficking enhancement.

Depositor

John Lin

Article

Mermet-Joret et al eLife 2023 12:RP90327

Insert

vfChrimson-citrine with membrane trafficking enhancement

Use

AAV

Mutation

vfChrimson with membrane trafficking enhancement

Promoter

Human Synapsin

Available Since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-EF1A-Gephyrin.FingR-eGFP-CCR5TC

Plasmid

#125692

Purpose

Global labeling of inhibitory synapses (green fluorescence)

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

Gephyrin.FingR-eGFP-CCR5TC

Use

AAV

Expression

Mammalian

Promoter

EF1A

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET-His-SCov2-nsp7

Plasmid

#154757

Purpose

Expression of SARS-CoV-2 nsp7

Depositor

Patrick Cramer

Article

Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

Insert

SARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-Lifeact-mKate2

Plasmid

#109493

Purpose

Muscle specific zebrafish expression of mKate2 fused to the actin binding lifeact sequence

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actc1b-Lifeact-mKate2

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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