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pAAV-DIO-SYP-HRP

Plasmid

#117186

Purpose

Plasmid name in publication: pAAV-DIO-SV-HRP. Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Article

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

SYP-HRP

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1a

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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aav-CAG-tdNfsB(F124W)-mCherry (eNTR)

Plasmid

#113761

Purpose

AAV-mediated expression of bacterial tdNfsB-mCherry (eNTR) under the CAG promoter

Depositor

Luke Lavis

Article

Gruber et al ACS Chem Biol. 2018 Aug 29. doi: 10.1021

Insert

tdNfsB(F124W)-mCherry

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

F124W

Promoter

CAG

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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CD4-C-GFP HDRT Source (pTR 152)

Plasmid

#112018

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with GFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CD4-GFP HDRT (CD4 Human)

Use

CRISPR and Synthetic Biology

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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sgPBRM1-2- LentiCRISPRv2

Plasmid

#107404

Purpose

Lentiviral expression of Cas9 and gRNA targeting PBRM1

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

gRNA targeting PBRM1 (PBRM1 Human)

Use

Lentiviral

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEM705 Gsi (Cys)

Plasmid

#109373

Purpose

GsX chimera with Gαi C-terminus (Pertussis toxin sensitive)

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gsi chimera

Tags

None

Expression

Mammalian

Mutation

Cys at position 391 (Pertussis Toxin Sensitive)

Promoter

CAG

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pSR49

Plasmid

#69259

Purpose

phlh-8::tagBFP2

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Insert

TagBFP2

Use

Cre/Lox

Expression

Worm

Mutation

codon-optimzed index 1.0, TagBFP2 contains 2 sing…

Promoter

hlh-8

Available Since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-V96 ELP

Plasmid

#67853

Purpose

GFP Fluorescent Elastin-like Polypeptide(VPGVG) with 96 repeats containing Valine guest residues

Depositor

Andrew MacKay

Article

Pastuszka et al Biomacromolecules. 2012 Nov 12;13(11):34

Insert

GFP fused to ELP (VPGVG) repeated 96 times

Tags

GFP fused to N-terminal of ELP

Expression

Mammalian

Promoter

T7

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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TNF-SBP-mCherry_puromycin

Plasmid

#65285

Purpose

TNF reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

TNF (TNF Human)

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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miR-34aP1

Plasmid

#50826

Purpose

luciferase reporter for human miR-34a

Depositor

Yun Bai, Xueqing Xu

Article

Li et al BMC Mol Biol. 2012 Jan 31;13:4.

Insert

miR-34A promoter (MIR34A Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

miR34a

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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pBRPyCAG-fmKlf4-DsRed-Ip

Plasmid

#26274

Depositor

Rudolf Jaenisch

Article

Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

Insert

Klf4 (Klf4 Mouse)

Use

Cre/Lox

Expression

Mammalian

Available Since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only

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pVETL-Cmcs-Flag-Ataxin3Q80

Plasmid

#22120

Depositor

Henry Paulson

Article

Todi et al J Biol Chem. 2007 Oct 5. 282(40):29348-5

Insert

Ataxin 3 (ATXN3 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
pL106P-LUC-ENTR1A(w451-1)

Plasmid

#17455

Purpose

Gateway entry vector, Degron N-term Luciferase

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

FKBP-Luciferase fusion (MTOR firefly)

Use

Gateway Cloning and Luciferase

Tags

FKBP12DD

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pSuper mouse DGCR8-3

Plasmid

#14774

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

DGCR8 shRNA (Dgcr8 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3-myc-MmUbc7/2776

Plasmid

#11488

Depositor

Allan Weissman

Article

Tiwari et al J Biol Chem. 2001 May 11. 276(19):16193-

Insert

MmUbc7 (Ube2g2 Mouse)

Tags

myc

Expression

Mammalian

Mutation

n/a

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
pLX317-RBFOX1

Plasmid

#115442

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

RBFOX1 (RBFOX1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-LifeAct-Venus

Plasmid

#87613

Purpose

Actin-targeted Venus (YFP).

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

LifeAct-Venus

Tags

17-aa sequence from Abp140 (aka "Lifeact&quo…

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pU6-(BbsI)sgRNA_CAG-Cas9-venus-bpA

Plasmid

#86986

Purpose

For cloning and expression of sgRNA together with expression of a Cas9-Venus fusion protein

Depositor

Ralf Kuehn

Article

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Insert

Cas9-venus

Tags

Cas9-Venus fusion protein

Expression

Mammalian

Promoter

CAG

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only
pPB-CAG-Tfe3::ERT2-pgk-hph

Plasmid

#48756

Purpose

PiggyBac backbone, CAG promoter driving murine Tfe3-ERT2 fusion, Hygromycin selection

Depositor

Austin Smith

Article

Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

Insert

Tfe3::ERT2 (Tcfe3 Mouse)

Use

Piggybac

Tags

ERT2

Expression

Mammalian

Promoter

CAG

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
GLDC-RES

Plasmid

#72882

Purpose

overexpression

Depositor

David Sabatini

Article

Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

Insert

GLDC (GLDC Human)

Use

Lentiviral

Expression

Mammalian

Mutation

silent mutations in site corresponding to shGLDC_2

Promoter

CMV

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only
ORAI1-hBACCS2

Plasmid

#67626

Purpose

Mammalian expression of ORAI1-hBACCS2, a fusion protein of ORAI1 and human blue light-activated Ca2+ channel switch

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

ORAI1-hBACCS2

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2015

Availability

Academic Institutions and Nonprofits only

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