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pAK004

Plasmid

#233641

Purpose

For bacterial expression of eSpCas9(1.1) c-terminally tagged with VitelloTagA.

Depositor

Zak Swartz

Article

Clarke et al Development. 2024 Oct 15;151(20):dev2028

Insert

eSpCas9(1.1) with c-terminal VitelloTagA

Tags

6-His

Expression

Bacterial

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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pCFD5_w

Plasmid

#112645

Purpose

Ubiquitous expression of one or multiple sgRNAs in Drosophila. Selection marker: Mini-white.

Depositor

Michael Boutros

Insert

U6:3-tRNA-sgRNA expression cassette

Expression

Insect

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pSUP-(2xCouRS-tRNAopt)

Plasmid

#197930

Purpose

Machinery plasmid expressing constitutive and inducible 7HCou synthetase and cognate amber suppressing tRNA. Incorporates (7-hydroxy-4-coumarin-yl) ethylglycine

Depositor

Kenneth Johnson

Article

Dangerfield et al J Biol Chem. 2020 Dec 11;295(50):17265-1

Inserts

CouRS
tRNA opt

Expression

Bacterial

Promoter

glnS' and lambda(ts) and proK

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1ab-Prkd1

Plasmid

#139314

Purpose

Diacylglycerol biosensor

Depositor

Gerry Hammond

Article

Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

mCherry:Mus musculus Prkd1(138-343) (Prkd1 Mouse)

Expression

Mammalian

Mutation

T208A, N313D

Available Since

April 8, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST53-LRRK2-G2019S

Plasmid

#25045

Depositor

Mark Cookson

Article

Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

Insert

LRRK2 (LRRK2 Human)

Tags

GFP

Expression

Mammalian

Mutation

Glycine 2019 mutated to Serine

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

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pLenti6.2-3xFLAG-V5-ccdB

Plasmid

#87072

Purpose

Lentiviral Gateway-compatible expression vector backbone with a N-terminal 3xFLAG-V5 tag

Depositor

Susan Lindquist, Mikko Taipale

Article

Taipale et al Cell. 2014 Jul 17;158(2):434-48. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

3xFLAG-V5

Expression

Mammalian

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-GATA2

Plasmid

#125028

Purpose

doxycycline-inducible expression of human GATA2 in mammalian cells

Depositor

Filipe Pereira

Article

Gomes et al Cell Rep. 2018 Dec 4;25(10):2821-2835.e7

Insert

GATA binding protein 2 (GATA2 Human)

Use

Lentiviral

Tags

None

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pICH41822

Plasmid

#48021

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pRSFDuet1_CoiA1_CoiB

Plasmid

#208761

Purpose

Precursor peptide CoiA1 and synthase CoiB, for the co-expression of CoiA1 in E.coli.

Depositor

Wilfred van der Donk

Article

Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

Inserts

CoiA1
CoiB

Tags

Hisx6 Tag and SUMO

Expression

Bacterial

Mutation

Changed Ser 20 to Asn, Changed Arg 84 to Lys, bot…

Promoter

T7

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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ABE8e-NG

Plasmid

#226856

Purpose

Expresses ABE8e-NG base editor in mammalian cells with eGFP marker

Depositor

Alexis Komor

Article

Osgood et al Methods in Enzymology, February 5, 2025

Insert

ABE8e-NG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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