We narrowed to 59,253 results for: {{{s}}}

1. pCMV-mGold-Mito-N-7

   Plasmid

   #158007

   Purpose

   Visualization of mitochondria in mammalian cells using mGold

   Depositor

   Francois St-Pierre

   Article

   Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

   Insert

   Mito (COX8A Human)
   

   Use

   Tags

   mGold

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3 c-SRC (151-536)

   Plasmid

   #42207

   Depositor

   Robert Lefkowitz

   Article

   Miller et al J Biol Chem. 2000 Apr 14;275(15):11312-9

   Insert

   c-SRC (151-536) (SRC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   contains amino acids 151-536

   Promoter

   CMV

   Available Since

   Feb. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pCAG-Bmpr2ΔKinase gRNA resistant

   Plasmid

   #163411

   Purpose

   Expresses BMPR-2ΔKinase resistant to the gRNAs(#163388-90) in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Bmpr2 (Bmpr2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   The kinase domain (202-500 aa) is deleted. gRNA-t…

   Promoter

   Available Since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSUPER-retro-puro-Smad4

   Plasmid

   #89829

   Purpose

   Knockdown human Smad4 expression in human mammalian cells

   Depositor

   Shengyu Yang

   Article

   Sun et al J Biol Chem. 2011 Nov 11;286(45):38865-7

   Insert

   Smad4 shRNA (SMAD4 Mouse, Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV CMV-driven AcrIIA4-2xmiR-122 target sites

   Plasmid

   #120298

   Purpose

   AAV vector for expression of AcrIIA4 with two miR-122 binding sites

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

   Insert

   AcrIIA4-2xmiR-122 binding sites
   

   Use

   AAV and CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

6. pDECKO_mCherry_non-targeting

   Plasmid

   #140109

   Purpose

   CRISPR-Cas9 library validation (negative control)

   Depositor

   Roderic Guigo

   Article

   Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   gRNA1 under U6 and gRNA2 under H1

   Available Since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEL030

   Plasmid

   #137899

   Purpose

   STU2.0 SpyCas9 Csy4 system for plant genome editing

   Depositor

   Yong Zhang

   Article

   Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Aug. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCS2+ Zf ctnna1L344P

   Plasmid

   #185407

   Purpose

   Zebrafish ctnna1 bearing a point mutation in the vinculin binding site. For in vitro transcription (SP6) or expression through CMV

   Depositor

   Nicolas David

   Article

   Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

   Insert

   ctnna1 (ctnna1 Zebrafish)
   

   Use

   In vitro synthesis of mrna

   Tags

   Expression

   Mammalian

   Mutation

   changed Leucine 344 to Proline

   Promoter

   Available Since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. TRP1MX6-ins4

   Plasmid

   #195041

   Purpose

   pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference

   Depositor

   Gloria Brar

   Article

   Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

   Insert

   TRP1
   

   Use

   Yeast genomic targeting

   Tags

   S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. SPACE-ΔUGI (pRZ1836)

    Plasmid

    #140243

    Purpose

    CMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    SPACE-ΔUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V82G in TadA*, D10A in Cas9, R187W in pmCDA1

    Promoter

    CMV

    Available Since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. XLone-Puro Cas13d-eGFP U6 SOX17 g2

    Plasmid

    #155188

    Purpose

    PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and SOX17 gRNA2

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biomaterials. 2022 May 6;285:121569. doi

    Insert

    Cas13d SOX17 gRNA2
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSI-359

    Plasmid

    #131131

    Purpose

    gRNA expression vector for A-to-G base editing reporter

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    A-to-G reporter activation gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEL033

    Plasmid

    #137902

    Purpose

    STU2.0 rAPOBEC1-SpyCas9(D10A) for plant genome base editing

    Depositor

    Yong Zhang

    Article

    Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-C1-IFT54

    Plasmid

    #218726

    Purpose

    Expresses N-terminally EGFP-tagged IFT54 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Hiyamizu et al J Cell Sci. 2023 Mar 1;136(5):jcs260462.

    Insert

    IFT54 (TRAF3IP1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEL029

    Plasmid

    #137898

    Purpose

    STU2.0 SpyCas9 RZ system for plant genome editing

    Depositor

    Yong Zhang

    Article

    Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1(+)-cpPKN-mRuby2-GFP1-10

    Plasmid

    #181859

    Purpose

    Part of FluoSTEP-RhoA biosensor; must be expressed with GFP11-tagged RhoA plasmid (Addgene #181860) or in GFP11-RhoA gene-edited cells.

    Depositor

    Jin Zhang

    Article

    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

    Insert

    cp-PKN-mRuby2-GFP(1-0)
    

    Use

    Tags

    mRuby2 and sfGFP(1-10)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKLV2-U6gRNA5(GFP)-PGKpuro2ABFP-W

    Plasmid

    #200475

    Purpose

    Lentiviral vector expressing gRNA targeting GFP

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. MAC-TMPRSS4

    Plasmid

    #172422

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

    Insert

    Transmembrane protease serine 4 (TMPRSS4 Human)
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available Since

    Nov. 4, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pcDNA3 c-SRC (91-536)

    Plasmid

    #42206

    Depositor

    Robert Lefkowitz

    Article

    Miller et al J Biol Chem. 2000 Apr 14;275(15):11312-9

    Insert

    c-SRC (91-536) (SRC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    contains amino acids 91-536

    Promoter

    CMV

    Available Since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pLV Reep3-V5

    Plasmid

    #175119

    Purpose

    Lentiviral expression of mouse Reep3-V5

    Depositor

    Larry Gerace

    Article

    Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

    Insert

    Reep3 (Reep3 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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