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pCAG-Golden-Gate-Esp3I-Destination

Plasmid

#51278

Purpose

CAG promoter-driven expression vector suitable for ligation of multiple inserts by Golden Gate Cloning using Esp3I

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Type

Empty backbone

Expression

Mammalian

Promoter

CAG

Available Since

March 4, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA-Golgi-ZapCY1

Plasmid

#36322

Depositor

Amy Palmer

Article

Qin et al Proc Natl Acad Sci U S A. 2011 May 3;108

Insert

Golgi-ZapCY1 high affinity Zn(II) sensor

Tags

GalT (first 60 amino acids)

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2012

Availability

Academic Institutions and Nonprofits only

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hTRIM65_EGFP_N1

Plasmid

#54320

Purpose

Mammalian expression vector for human TRIM65 fusion protein with c-terminal EGFP.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

TRIM65 (TRIM65 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2014

Availability

Academic Institutions and Nonprofits only

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FUW-KM

Plasmid

#20332

Depositor

Rudolf Jaenisch

Article

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Klf4-F2A-cMyc

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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854-Desmocollin 1a-myc

Plasmid

#32231

Depositor

Kathleen Green

Article

Ishii et al J Invest Dermatol. 2001 Jul;117(1):26-35

Insert

Desmocollin 1a (DSC1 Human)

Tags

Myc

Expression

Mammalian

Promoter

human beta-actin

Available Since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only
pBABE-puro-NLS-LSSmKate2

Plasmid

#34586

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

NLS-LSS-mKate2

Use

Retroviral

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

Available Since

Feb. 21, 2012

Availability

Academic Institutions and Nonprofits only

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HSD2-FLAG

Plasmid

#24097

Depositor

Moses Chao

Article

Jeanneteau et al Proc Natl Acad Sci U S A. 2008 Mar 25. 1

Insert

HSD2 (HSD11B2 Human)

Use

Lentiviral

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-D1cpV

Plasmid

#37479

Depositor

Amy Palmer, Roger Tsien

Article

Palmer et al Proc Natl Acad Sci U S A. 2004 Dec 14;10

Insert

D1cpV second generation cameleon (calcium sensor)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2012

Availability

Academic Institutions and Nonprofits only
pRAF-MutH

Plasmid

#86609

Purpose

E. coli MutH expression plasmid

Depositor

Richard Fishel

Article

Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

Insert

E. coli MutH

Tags

his-tag and ald-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pRAF-MutS

Plasmid

#86605

Purpose

E. coli MutS expression plasmid

Depositor

Richard Fishel

Article

Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

Insert

E. coli MutS (D835R,R840E)

Tags

his-tag and ald-tag

Expression

Bacterial

Mutation

D835R, R840E

Promoter

T7

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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Fus-BcLOV-mCh

Plasmid

#208277

Purpose

Expresses Fus-BcLOV fusion for optogenetic membrane recruitment. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Article

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

Fus-BcLOV-mCh

Expression

Mammalian

Promoter

pCMV

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CamKII-DIO-mCherry

Plasmid

#190241

Purpose

Expresses mCherry flanked by DIO

Depositor

Young J Yoon

Article

Nasrallah et al Proc Natl Acad Sci U S A. 2022 Aug 9;119

Insert

mCherry

Use

AAV

Tags

N/A

Promoter

mouse CamKII alpha

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pKPY514

Plasmid

#62598

Purpose

Encodes Thr251Gly-EcPheRS Under IPTG-Inducible (PT5) Control

Depositor

David Tirrell

Article

Yuet et al Proc Natl Acad Sci U S A. 2015 Feb 17. p

Insert

Thr251Gly-EcPheRS

Tags

6xHis

Expression

Bacterial

Mutation

Thr251Gly

Promoter

T5

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pRLuc110UGA

Plasmid

#41729

Depositor

James Inglese

Article

Auld et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

RLuc110UGA

Use

Luciferase

Expression

Mammalian

Mutation

in-frame UGA stop codon at codon 110 in the open …

Promoter

CMV

Available Since

June 20, 2013

Availability

Academic Institutions and Nonprofits only
FLM-PC1

Plasmid

#41567

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Tags

CD16.7

Expression

Mammalian

Mutation

sequence from 4078 - 4303 of PKD1

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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HSD11B1-FLAG

Plasmid

#24096

Depositor

Moses Chao

Article

Jeanneteau et al Proc Natl Acad Sci U S A. 2008 Mar 25. 1

Insert

HSD11beta1 (HSD11B1 Human)

Use

Lentiviral

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only
VV020: WT Arch-3 - eGFP in pET28b

Plasmid

#58488

Purpose

Expresses WT Archaerhodopsin-3 fused to eGFP (with 6x C-terminal His tag) in E. coli

Depositor

Adam Cohen

Article

Maclaurin et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

Insert

Archaerhodopsin-3

Tags

eGFP

Expression

Bacterial

Promoter

T7

Available Since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pNOC-stacked-aequorin

Plasmid

#101010

Purpose

Construct for expression of aequorin, calcium sensitive luciferase reporter and hygromycin resistance gene by a bidirectional promoter

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis

Promoter

Ribi

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pKI1

Plasmid

#199118

Purpose

Expresses N-terminally Hisx6-tagged, SNAP-tagged full-length RapA in E. coli cells, suitable for fluorescent labelling

Depositor

Jeff Gelles

Article

Inlow et al Proc Natl Acad Sci U S A. 2023 Jul 11;12

Insert

H6-SNAP-RapA

Tags

6xHis and SNAP-tag

Expression

Bacterial

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Y-RAN-GFP26

Plasmid

#106423

Purpose

Y-RAN-GFP26 (RANbody GFP26::Chicken IgY)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

Y-RAN-GFP26

Tags

HA and His

Expression

Mammalian

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pPRIME-CMV-GFP-recipient

Plasmid

#11657

Purpose

3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with CMV promoter controlling GFP expression

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Type

Empty backbone

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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pDisplay FLIPE-600n

Plasmid

#13545

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E WT

Tags

ECFP and Venus

Expression

Mammalian

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pCTcon2-5INS_16

Plasmid

#102292

Purpose

Yeast Surface Display of 5Ins_16 scFV

Depositor

Sarel Fleishman

Article

Baran et al Proc Natl Acad Sci U S A. 2017 Oct 10;11

Insert

Insulin binding scFV

Tags

3x(G4S) Linker, G3S Linker, HA, and Myc

Expression

Yeast

Promoter

GAL1

Available Since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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DdMCU

Plasmid

#67642

Purpose

DdMCU-Flag in pACT2 vector human codon optimised

Depositor

Vamsi Mootha

Article

Kovacs-Bogdan et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

DdMCU

Tags

Flag tag with linker

Expression

Yeast

Promoter

ADH1

Available Since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-ZapCY2

Plasmid

#36320

Depositor

Amy Palmer

Article

Qin et al Proc Natl Acad Sci U S A. 2011 May 3;108

Insert

ZapCY2 high affinity Zn(II) sensor

Expression

Mammalian

Mutation

see comment

Promoter

CMV

Available Since

May 18, 2012

Availability

Academic Institutions and Nonprofits only
pRLuc190UGA

Plasmid

#41047

Depositor

James Inglese

Article

Auld et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

RLuc190UGA

Use

Luciferase

Expression

Mammalian

Mutation

in-frame UGA stop codon at codon 190 in the open …

Promoter

CMV

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
NTS-PC1

Plasmid

#41574

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Tags

CD16.7 and Myc/Myc/His

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pSL-1

Plasmid

#180422

Purpose

Kan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes a low level RFP promoter.

Depositor

Nancy Moran

Article

Li et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

Red Chromprotein gene

Expression

Bacterial

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pFLAG-CMV4-ZIKV-NS4A

Plasmid

#178845

Purpose

Mammalian expression of ZIKV ORF

Depositor

Judy Lieberman

Article

Sen Santara et al Proc Natl Acad Sci U S A. 2021 Nov 23;11

Insert

ZIKV-NS4A

Expression

Mammalian

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pFLAG-CMV4-ZIKV-NS3

Plasmid

#178844

Purpose

Mammalian expression of ZIKV ORF

Depositor

Judy Lieberman

Article

Sen Santara et al Proc Natl Acad Sci U S A. 2021 Nov 23;11

Insert

ZIKV-NS3

Expression

Mammalian

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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