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Plasmid#60152PurposeFirefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by LEU1 promoter.DepositorInsertFLuc-yEVenus-PEST
UseTagsPEST degronExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterLEU1prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB789
Plasmid#60166PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by RNR1 promoter.DepositorInsertFLuc-yEVenus-PEST
UseTagsPEST degronExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterRNR1prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pet23a_RBPMS_20_101
Plasmid#65733Purposeplasmid for bacterial overexpression of RBPMSDepositorInsertRBPMS (RBPMS Human)
UseTagsHis TagExpressionBacterialMutationdeletion construct encoding amino acids 20-101PromoterT7Available sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR4_FUS_G2A_R521H
Plasmid#26370DepositorInsertFUS (FUS Human)
UseEntry vectorTagsExpressionMutationR521HPromoterAvailable sinceSept. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHR-LS
Plasmid#205438PurposeExpression of Gaussia luciferase (gLuc) and spectinomycin resistance (Spec) genesDepositorInsertsGaussia luciferase gene
spectinomycin resistance gene
UseCre/Lox and Luciferase; Chlamydomonas expressionTagsExpressionMutationPromoterHeat shock protein 70A (HSP70A)/ribulose bisphosp…Available sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJUMP24_T24_pRham-ilvA-relE_RBS3_CB2
Plasmid#201533PurposeExpressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)DepositorInsertilvA-relE_RBS3_CB2
UseSynthetic BiologyTagsExpressionMutationrhaR L128+1bp (frameshift), ilvA G455CPromoterPrhaBAD (rhamnose)Available sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJUMP24_T24_pRham-ilvA-relE_RBS3_CB4
Plasmid#201535PurposeExpressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)DepositorInsertilvA-relE_RBS3_CB4
UseSynthetic BiologyTagsExpressionMutationrhaS ΔM1-H5, PrhaSR Δ179bpPromoterPrhaBAD (rhamnose)Available sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
ptAD-seq-ubi63E-Gal4-DBD
Plasmid#111930PurposeVector to express tAD candidates from a library cloned from fragmented transcription factor coding sequences fused to the Gal4 DNA binding domain.DepositorInserttAD-seq Gal4-DBD
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsDCP1a-withDmTD_H
Plasmid#146504PurposeMammalian Expression of HsDcp1a-withDmTDDepositorInsertHsDcp1a-withDmTD (DCP1A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsDCP1a-withDmTD_H
Plasmid#146506PurposeMammalian Expression of HsDCP1a-withDmTDDepositorInsertHsDCP1a-withDmTD (DCP1A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-HsDCP1a_539-582 (MCSII)-mut1_G
Plasmid#146408PurposeBacterial Expression of HsDCP1a_539-582-mut1DepositorInsertHsDCP1a_539-582-mut1 (DCP1A Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-HsDCP1a_539-582 (MCSII)-mut3_G
Plasmid#146410PurposeBacterial Expression of HsDCP1a_539-582-mut3DepositorInsertHsDCP1a_539-582-mut3 (DCP1A Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsDCP1a_539-582_G
Plasmid#146412PurposeMammalian Expression of HsDcp1a_539-582DepositorInsertHsDcp1a_539-582 (DCP1A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsDCP1a-del155-168_G
Plasmid#146413PurposeMammalian Expression of HsDcp1a-del155-168DepositorInsertHsDcp1a-del155-168 (DCP1A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsDCP1a-del539-582_G
Plasmid#146414PurposeMammalian Expression of HsDcp1a-del539-582DepositorInsertHsDcp1a-del539-582 (DCP1A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsDCP1a-mut2_G
Plasmid#146416PurposeMammalian Expression of HsDCP1a-mut2DepositorInsertHsDCP1a-mut2 (DCP1A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsDCP1a-mut3_G
Plasmid#146417PurposeMammalian Expression of HsDCP1a-mut3DepositorInsertHsDCP1a-mut3 (DCP1A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmEDC3-4mut-siRNAres_E
Plasmid#146193PurposeInsect Expression of DmEDC3-4mut-siRNAresDepositorInsertDmEDC3-4mut-siRNAres (Edc3 Fly)
UseTagsExpressionInsectMutationone silent mutation A456G and three mutations N16…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmEDC3-5mut-siRNAres_E
Plasmid#146194PurposeInsect Expression of DmEDC3-5mut-siRNA resDepositorInsertDmEDC3-5mut-siRNA res (Edc3 Fly)
UseTagsExpressionInsectMutationone silent mutation A456G and three mutations N16…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmEDC3-ADF_E
Plasmid#146195PurposeInsect Expression of DmEDC3-ADFDepositorInsertDmEDC3-ADF (Edc3 Fly)
UseTagsExpressionInsectMutationone silent mutation A456G and three mutations N16…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only