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pCI-BCD7-lacI-ssrA P62U18C50T16 v40-4a (496)

Plasmid

#70737

Purpose

plasmid for building 5n1

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

lacI-ssrA

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only

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pQacR-BCD7-tetR-ssrA P68U18C45T13 v40-5 (569)

Plasmid

#70739

Purpose

plasmid for building 5n2

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

tetR-ssrA

Available Since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pSrpR-BCD20-Cerulean-ssrA P65U23C89T19 v50-2a (587)

Plasmid

#70744

Purpose

for building 3-color reporter plasmid

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

Cerulean-ssrA

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pSrpR-BCD7-betI-ssrA P65U18C64T14 v50-2 (567)

Plasmid

#70734

Purpose

plasmid for building 3n1, 5n2

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

betI-ssrA

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pBetI-BCD7-qacR-ssrA P69U18C63T16 v50-2 (568)

Plasmid

#70732

Purpose

plasmid for building 5n2

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

phlF-ssrA

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pBetI-BCD7-phlF-ssrA P69U18C51T15 v40-3 (570)

Plasmid

#70731

Purpose

plasmid for building 3n1

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

phlF-ssrA

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-BCD7-Citrine P62U18C68T11 v40-1 (523)

Plasmid

#70726

Purpose

Citrine reporter for transfer fxns

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

Citrine

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pBetI-BCD7-Citrine P69U18C68T11 v40-1 (532)

Plasmid

#70723

Purpose

Citrine reporter for transfer fxns

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

Citrine

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX mPtch-Cterm

Plasmid

#58704

Purpose

used for anti-mouse Patched antibody production (for injection into animals)

Depositor

Matthew Scott

Article

Rohatgi et al Science. 2007 Jul 20;317(5836):372-6.

Insert

Ptch (Ptch1 Mouse)

Tags

GST

Expression

Bacterial

Mutation

contains only Cterminus (aa ~1238-1413)

Promoter

tac

Available Since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only
BS.YxiN.Twin1.a1-368.wt

Plasmid

#13528

Depositor

David McKay

Article

Karginov et al J Biol Chem. 2005 Oct 21. 280(42):35499-

Insert

B. subtilis DEAD-box helicase YxiN first two domains

Tags

intein-chitin binding domain

Expression

Bacterial

Mutation

deleted amino acid residues 369-479

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

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TM1442-pET-21a

Plasmid

#12650

Depositor

SeWon Suh

Article

Lee et al Proteins. 2004 Jul 1. 56(1):176-9.

Insert

Putative anti-sigma factor antagonist (TM1442 T. maritima)

Expression

Bacterial

Mutation

N/A

Available Since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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BS.YxiN.Twin1.a404-479.wt

Plasmid

#13529

Depositor

David McKay

Article

Karginov et al J Biol Chem. 2005 Oct 21. 280(42):35499-

Insert

B. subtilis DEAD-box helicase YxiN specific RNA binding domain

Tags

intein-chitin binding domain

Expression

Bacterial

Mutation

delete amino acid residues 1-403

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

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pLIB-RSP1

Plasmid

#176690

Purpose

Expression of Chlamydomonas reinhardtii RSP1 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP1

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

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pLIB-RSP2

Plasmid

#176691

Purpose

Expression of Chlamydomonas reinhardtii RSP2 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP2

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

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pLIB-RSP6

Plasmid

#176695

Purpose

Expression of Chlamydomonas reinhardtii RSP6 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP6

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-RSP7

Plasmid

#176696

Purpose

Expression of Chlamydomonas reinhardtii RSP7 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP7

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-RSP9

Plasmid

#176697

Purpose

Expression of Chlamydomonas reinhardtii RSP9 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP9

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

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pLIB-RSP10

Plasmid

#176698

Purpose

Expression of Chlamydomonas reinhardtii RSP10recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP10

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-RSP11

Plasmid

#176699

Purpose

Expression of Chlamydomonas reinhardtii RSP11 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP11

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

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pLIB-RSP12

Plasmid

#176700

Purpose

Expression of Chlamydomonas reinhardtii RSP12 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP12

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge