We narrowed to 5,214 results for: GST

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pGEX-4T-1 3xHA-MEK1-DD

Plasmid

#47576

Purpose

Bacterial expression plasmid for GST-3xHA-tagged constitutively active MEK1

Depositor

Kevin Janes

Article

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Insert

MEK1-DD (Map2k1 Mouse)

Tags

3xHA and GST

Expression

Bacterial

Mutation

Ser218 to Asp, Ser221 to Asp

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
pGEX-4T-1 3xHA-MKK6-EE

Plasmid

#47579

Purpose

Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK6

Depositor

Kevin Janes

Article

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Insert

MKK6-EE (MAP2K6 Human)

Tags

3xHA and GST

Expression

Bacterial

Mutation

Ser207 to Glu, Thr211 to Glu

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
pGEX4T-1-Optimized E6AP

Plasmid

#165100

Purpose

ecoli optimized expression of GST-fusion of human E6AP

Depositor

Arthur Haas

Article

Ronchi et al J Biol Chem. 2013 Apr 12;288(15):10349-6

Insert

E6AP isoform 3 (UBE3A Human)

Tags

GST from plasmid.

Expression

Bacterial

Mutation

codon optimized for E. coli expression

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-SUMO1

Plasmid

#185637

Purpose

expression of GST-tagged SUMO1 in bacterial cells

Depositor

Irina Bezsonova

Article

Bregnard et al Biophys Chem. 2022 Aug;287:106827. doi:

Insert

small ubiquitin-like modifier 1 (SUMO1 Human)

Tags

GST

Expression

Bacterial

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pYO1125

Plasmid

#235742

Purpose

Protein expression of GST-tagged PX domain (Cys at 2) in bacteria

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

p40 PX domain (NCF4 Human)

Tags

GST-TEV

Expression

Bacterial

Mutation

aa 2-149 with Cys at 2

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pETM33_PDCD6IP_ALIX V-domain

Plasmid

#178483

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

PDCD6IP_ALIX V-domain (PDCD6IP Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-2T_EV-D68_VP1

Plasmid

#202072

Purpose

Bacterial expression of VP1 protein from enterovirus D68 (EV-D68). Contains N-terminal GST-tag and C-terminal His6-tag.

Depositor

Vesa Hytönen

Article

Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

Insert

EV-D68 VP1 protein with Histag

Tags

6xHis-tag and GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-2/hGGA3(GAT)

Plasmid

#79436

Purpose

Expresses GST-tagged GAT domain of GGA3 (107-286 aa) in E.coli

Depositor

Kazuhisa Nakayama

Article

Takatsu et al Biochem J. 2002 Jul 15;365(Pt 2):369-78.

Insert

GGA3 (GGA3 Human)

Tags

GST

Expression

Bacterial

Mutation

amino acids 107-286

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-optimizedNedd4.2d

Plasmid

#165103

Purpose

bacterial expression of a GST fusion of Human Nedd4.2d

Depositor

Arthur Haas

Article

Todaro et al J Biol Chem. 2017 Nov 24;292(47):19521-1

Insert

Nedd4.2d (NEDD4L Human)

Tags

GST from plasmid.

Expression

Bacterial

Mutation

codon optimized for E. coli expression

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-2/hGGA1(GAT)

Plasmid

#79435

Purpose

Expresses GST-tagged GAT domain of GGA1 (141-327 aa) in E.coli

Depositor

Kazuhisa Nakayama

Article

Takatsu et al Biochem J. 2002 Jul 15;365(Pt 2):369-78.

Insert

GGA1 (GGA1 Human)

Tags

GST

Expression

Bacterial

Mutation

amino acids 141-327

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pBacPAK p110a RBD DM

Plasmid

#55723

Purpose

His3 GST tagged RBD double mutant p110 alpha T207D, K227A

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

p110 alpha (Pik3ca Mouse)

Tags

His3 GST

Mutation

T207D, K227A

Promoter

polyhedrin promoter

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pGEX-6P-2_dCrk

Plasmid

#131146

Purpose

Expresses GST::dCrk (based on crk isoform RA).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

Crk (Crk Fly)

Tags

GST

Expression

Bacterial

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX2T VPS4B, residues 1-86

Plasmid

#180639

Purpose

Bacterial expression for VPS4B MIT domain, residues 1-86. Has N-terminal GST tag. Internal ID: WISP04-155.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

VPS4B (VPS4B Human)

Tags

GST

Expression

Bacterial

Mutation

Residues 1-86

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6p-hDcp2 E147/8Q

Plasmid

#72217

Purpose

bacterial expression of GST-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)

Depositor

Megerditch Kiledjian

Article

Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

Insert

Dcp2 (DCP2 Human)

Tags

GST

Expression

Bacterial

Mutation

E147Q, E148Q and F404L (please see depositor'…

Promoter

tac

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
pGEX-SF3B1-NTerm

Plasmid

#97425

Purpose

Expresses human SF3B1 (AA 1-500) with N-term GST tag for inducible expression in E.coli

Depositor

Alex Minella

Article

Murthy et al J Biol Chem. 2018 May 15. pii: RA118.001

Insert

SF3B1-N-term (AA1-500)

Tags

GST

Expression

Bacterial

Mutation

Silent mutation at nt876, aa289 (AGG to AGA)

Promoter

tac

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pETM33_ZYMND11_coiled-coil MYND

Plasmid

#178493

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

ZYMND11_coiled-coil MYND (ZMYND11 Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pETM33_PTK2_Focal-AT

Plasmid

#178485

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

PTK2_Focal-AT (PTK2 Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX6P1 UBXN11

Plasmid

#169017

Purpose

Expresses GST-UBXN11 (human) in bacteria

Depositor

Hemmo Meyer

Article

Kracht et al J Mol Biol. 2020 Nov 20;432(23):6061-607

Insert

UBXN11 (UBXN11 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX4T1-ORF24-NTD

Plasmid

#138464

Purpose

Expresses GST-tagged ORF24-NTD in bacteria

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Tags

GST

Expression

Bacterial

Mutation

Amino acids 1-201

Promoter

tac

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX6P1‐Afu‐rnhBΔPIP

Plasmid

#108696

Purpose

For expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with C-terminal PIP box deletion

Depositor

Andrew Jackson, Martin Reijns

Article

Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

Insert

rnhB

Tags

GST

Expression

Bacterial

Mutation

C-terminal truncation (7aa) removing PIP box

Promoter

tac

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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