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Plasmid#205988PurposeVector containing FRT and FRT-F3 sitesDepositorTypeEmpty backboneUsePcr vectorTagsExpressionMutationPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only
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pCHAP8
Plasmid#206857PurposeA plasmid containing a 11,944 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP7
Plasmid#206853PurposeA plasmid containing a 13,748 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPt_Sap
Plasmid#206856PurposeA plasmid containing the whole P. tricornutum chloroplast genome, cloned with a pCC1BAC-based backbone. It contains a single SapI site located in the URA3 marker, which was integrated into the genome.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP6
Plasmid#206852PurposeA plasmid containing a 15,671 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP5
Plasmid#206851PurposeA plasmid containing a 17,639 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP3_URA
Plasmid#206848PurposeA plasmid containing a 17,271 bp fragment of the P. tricornutum chloroplast genome. The yeast marker URA3 was integrated into the chloroplast fragment, which is flanked by SapI sites.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpH-CtDCP2_1-267_X
Plasmid#148004PurposeBacterial Expression of CtDCP2_1-267DepositorInsertCtDCP2_1-267
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NvH-CtNot1_925-2193_X
Plasmid#147972PurposeBacterial Expression of CtNot1_925-2193DepositorInsertCtNot1_925-2193
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNB649
Plasmid#60136PurposeFirefly luciferase (Promega) driven by MET17 promoter.DepositorInsertFLuc
UseTagsExpressionYeastMutationA4VPromoterMET17prAvailable sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-blast-cfRab8b_1
Plasmid#26708DepositorInsertdog Rab8b RNAi
UseLentiviral and RNAiTagsExpressionMammalianMutationDog Rab8b RNAiPromoterAvailable sinceDec. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
mCherry- SEC61B
Plasmid#121160PurposeMammalian expression of mCherry tagged SEC61B, used as a general ER markerDepositorInsertSEC61B (SEC61B )
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET15b-AavLEA1
Plasmid#53093PurposeAavLEA1 expression (in BL21 DE3 E. coli strain)DepositorInsertAavLEA1
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceJune 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-blast
Plasmid#26655Purpose3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses blasticidin for selection.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMV306G13+FFluc
Plasmid#26157DepositorInsertG13 promoter + Firefly luciferase
UseMycobacteria integrating vectorTagsExpressionMutationCodon optimized for Mycobacterium tuberculosis. …PromoterAvailable sinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pNOC-ARS-stacked-NeoR-mNeonGreen
Plasmid#101249PurposeConstruct for fusion expression of mNeonGreen reporter and G418 resistance gene by a bidirectional promoterDepositorTypeEmpty backboneUseAlgae, nannochloropsisTagsExpressionMutationPromoterRibiAvailable sinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
p426MET25_sfpHluorin (MRV55)
Plasmid#115697Purposesuperfolder pHluorin for intracellular pH measurements in S. cerevisiae (improved performance when targeted to the ER or subsequent compartments)DepositorInsertsfpHluorin
UseTagsExpressionYeastMutationfor introduced mutations see publicationPromoterMET25Available sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
NES-mCherry-P4Mx1
Plasmid#108143PurposePI4P biosensorDepositorInsertX.leavis map2k1.L(32-44):mCherry:L. penomophila SidM(546-647)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
5HT6-mCherry-G-GECO1.0
Plasmid#47500PurposeFluorescent reporter for Ca2+ inside primary cilia with mCherry referenceDepositorInsertG-GECO1.0
UseTags5HT6 and mCherryExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMC-1-StUbi-6
Plasmid#197710PurposePhytobrick (MoClo) Level 0 PartDepositorInsertStUbi promoter
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only