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iDamID plasmid. To stably and ubiquitously express the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
circular permutation-based fluorescence resonance energy transfer (cpFRET) biosensor toolkit: generate and optimize genetically encoded, unimolecular FRET sensors for ratiometric measurements
Ready-to-use Lentiviral Prep particles produced from Human CRISPRi sgRNA library Dolcetto in backbone XPR_050, Set A (#92385). In addition to the viral particles, you will also receive purified Human CRISPRi sgRNA library Dolcetto in backbone XPR_050, Set A plasmid DNA.
Ready-to-use AAV Retrograde particles produced from pAAV-Syn-ChR2(H134R)-GFP (#58880). In addition to the viral particles, you will also receive purified pAAV-Syn-ChR2(H134R)-GFP plasmid DNA.
Humanized channelrhodopsin H134R mutant fused to GFP, under the control of the Synapsin promoter.
These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
Ready-to-use AAV1 particles produced from pAAV.hSyn1.Twitch2B.WPRE.SV40 (#100040). In addition to the viral particles, you will also receive purified pAAV.hSyn1.Twitch2B.WPRE.SV40 plasmid DNA.
Synapsin-driven expression of (FRET)-based calcium biosensor Twitch2B.
These AAV preparations are suitable purity for injection into animals.