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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 31,303 results for: grna

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Showing: 1141 - 1160 of 31303 results

  1. LentiGuide-10X-CS1-3'-puro

    Plasmid
    #212011
    Purpose
    Deliver gRNA through lentivirus (compatible with 10x capture Sequence 1)
    Depositor
    Danwei Huangfu
    Insert
    gRNA scaffold
    Use
    Lentiviral
    Available since
    March 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    212011-plasmid-map-sequence-id-419682

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  9. PB-sgRNA(MS2)

    Plasmid
    #102560
    Purpose
    Piggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.
    Depositor
    Ying Liu
    Publication
    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    102560-plasmid-map-sequence-id-198595

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  11. eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA

    Plasmid
    #86613
    Purpose
    Vector for tandem expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G3 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via hdr using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    9 Articles
    86613-plasmid-map-sequence-id-168851

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  20. pDIRECT_25H

    Plasmid
    #91145
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers
    Use
    CRISPR
    Expression
    Plant
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    91145-plasmid-map-sequence-id-182281

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Showing: 1141 - 1160 of 31303 results