We narrowed to 23,079 results for: ESC

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AA334

Plasmid

#216068

Purpose

Fragmid fragment: (2A Selection) Stop; IRES2; Start; BlastR; Stop

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Stop; IRES2_v1; Start; BlastR_v1.1; Stop

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRDB_051

Plasmid

#216081

Purpose

for stable fly cell lines; attL and attR sequences for genome integration by phiC31, destination vector

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

attL and attR sequences for genome integration by phiC31

Use

Destination

Expression

Insect

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA151

Plasmid

#215938

Purpose

Fragmid fragment: (guide cassette) for dual expression sgRNA

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; BsmBI_v0; BsmBI_v4; rev{H1_v1}

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA375

Plasmid

#216054

Purpose

Fragmid fragment: (C' terminus) HIV nuclear export sequence; HA tag

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

HIV-NES_v1.1; HIV-NES_v1.2; 3xHA_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA400

Plasmid

#215958

Purpose

Fragmid fragment: (guide cassette) guide expression

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; DR_v1 [Rfx]; BsmBI_v1; BsmBI_v12

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA401

Plasmid

#215959

Purpose

Fragmid fragment: (guide cassette) guide expression

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; DR_v1 [Dj]; BsmBI_v1; BsmBI_v12

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA048

Plasmid

#216016

Purpose

Fragmid fragment: (Cas protein) deactivated Cas

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

dCas12a (D908A)_v1.1 [EnAs]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRDB_131

Plasmid

#216095

Purpose

Cas12a [EnAs] CRISPRa targeting CD97, CD4, CD26, CD274, positive control

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD97, CD4, CD26, CD274 guides

Use

CRISPR and Lentiviral; Positive controls

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRDA_816

Plasmid

#216098

Purpose

CRISPRa, EF1a-driven ALFA-dCas12a-VP64 (Cas only)

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

Cas12a [EnAs]

Use

CRISPR and Lentiviral; Assembled vector

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-mScarlet-I-eDHFR-ORP5(ΔPH)

Plasmid

#214277

Purpose

Mammalian expression of ORP5(ΔPH) fused to mScarlet-I-tagged E. coli DHFR

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

mScarlet-I-eDHFR-ORP5(253-879) (OSBPL5 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-myr-rsEGFP2- LDLR

Plasmid

#213399

Purpose

double floxed, myristoylation site (myr) and LDLR, fused to reversibly switchable EGFP rsEGFP2

Depositor

Katrin Willig

Article

Willig et al Cell Rep. 2021 Jun 1;35(9):109192. doi:

Insert

rsEGFP2

Use

AAV

Tags

C-terminal (Ct) cytoplasmic domains of low densit…

Expression

Mammalian

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_EF1a_DIO_HA/FLAG_muMASS_LF

Plasmid

#213394

Purpose

Loss of function variant of uMASS_1 opioid sensor. AAV virus production for Cre dependent expression.

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

uMASS_LF

Use

AAV and Cre/Lox

Expression

Mammalian

Available Since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_hsyn_HA_Flag-uMASS_LF

Plasmid

#209761

Purpose

AAV virus production for neuonal expression of uMASS (loss-of-function) under hSyn promoter

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

uMASS_LF

Use

AAV

Expression

Mammalian

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pNKX2-5-GFP-T2A

Plasmid

#203608

Purpose

Donor plasmid to create 5' knockin of NKX2-5 with GFP-T2A

Depositor

Matthew Birket

Article

Douglas et al Stem Cell Res. 2023 Dec 3;74:103262. doi

Insert

NKX2-5-GFP-T2A-NKX2-5 (NKX2-5 Human)

Use

Human targeting

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pET30b jGCaMP8f L27A

Plasmid

#204138

Purpose

Production of recombinant calcium sensor jGCaMP8f L27A fast decay variant in E.coli

Depositor

Katalin Torok

Article

Tran et al Front Cell Neurosci. 2023 May 18;17:1155

Insert

jGCaMP8f L27A

Tags

N-terminal His tag

Expression

Bacterial

Mutation

Leucine 27 changed to Alanine

Promoter

T7

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-dMASS_LF_WPRE

Plasmid

#209766

Purpose

AAV virus production for neuronal expression of dMASS_LF (loss-of-function) under hSyn promoter

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

dMASS_LF

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_hsyn_HA-Flag-uMASS_1_WPRE

Plasmid

#209760

Purpose

AAV virus production for neuronal expression of uMASS_1 under hSyn promoter

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

uMASS_1

Use

AAV

Expression

Mammalian

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-R-iLACCO1.2

Plasmid

#208021

Purpose

Biosensor for intracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

R-iLACCO1.2

Expression

Bacterial

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-R-diLACCO1

Plasmid

#208022

Purpose

Control biosensor for intracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

R-diLACCO1

Expression

Bacterial

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-deLACCO1

Plasmid

#208018

Purpose

Control biosensor for extracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

deLACCO1

Expression

Bacterial

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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