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pcDNA3.2/NS4B

Plasmid

#120823

Purpose

Ectopic expression of ZIKV protein

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

NS4B ZIKV

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF3_P2A_Hygro_Barcode

Plasmid

#120490

Purpose

Barcoded lentiviral vector to express KLF3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF3 (KLF3 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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FUW-hR3-EGFP

Plasmid

#119231

Purpose

Plasmid encodes full-length human VEGFR-3 gene fused in-frame with EGFP cloned in lentiviral vector.

Depositor

Kari Alitalo

Insert

VEGFR3-EGFP (FLT4 Human)

Use

Lentiviral

Tags

EGFP

Promoter

P hUbC

Available Since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pLIC-SM-Myc

Plasmid

#111189

Purpose

High affinity Myc tagging of weakly expressed genes (optimized for Toxoplasma gondii)

Depositor

Silvia Moreno

Article

Hortua Triana et al J Eukaryot Microbiol. 2018 Apr 19. doi:

Type

Empty backbone

Use

Toxoplasma gondii expression

Tags

Spaghetti Monster Myc Tag

Promoter

Endogenous

Available Since

Aug. 2, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pEM705 Gs15

Plasmid

#109360

Purpose

GsX chimera with Gα15 C-terminus

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gs15 chimera

Tags

None

Expression

Mammalian

Promoter

CAG

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS-6020nes

Plasmid

#108656

Purpose

A JNK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

Depositor

Michiyuki Matsuda

Article

Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

Insert

hyBRET-JNK-EV

Tags

XFP

Expression

Mammalian

Available Since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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pLJM1-EGFP-BarcodeV2

Plasmid

#78632

Purpose

GESTALT V2 integrating barcode with Puro and EGFP

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT V2 insert

Use

Lentiviral

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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PT7-deGFP-MGapt

Plasmid

#67741

Purpose

T7 promoter expressing deGFP-MG aptamer fusion

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-deGFP-MGapt-T7term

Use

Synthetic Biology

Expression

Bacterial

Promoter

PT7

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pJZC595

Plasmid

#62322

Purpose

Expresses MCP-VP64 and dCas9 in Yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

MCP-VP64
dCas9

Tags

VP64

Expression

Yeast

Mutation

Has nuclease-inactivating mutations in the RuvC1 …

Promoter

Tdh3 and pAdh

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pICH51288

Plasmid

#50269

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter (double), 35s (Cauliflower Mosaic Virus) + 5'UTR, omega (Tobacco Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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BAZ2B

Plasmid

#39000

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

BAZ2B (BAZ2B Human)

Tags

His Tev

Expression

Bacterial

Mutation

codon-optimized

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pENTR/pTER shEGFP #1 (628-2)

Plasmid

#17470

Purpose

Gateway entry vector for EGFPshRNA#1 under H1/TO promoter

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Enhanced green fluorescent protein shRNA #1

Use

Gateway Cloning and RNAi

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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MXS_TagBFP

Plasmid

#62400

Purpose

MXS_chaining vector with TagBFP

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

TagBFP

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HaloAKAR2.2-T2A-EGFP

Plasmid

#216490

Purpose

Expression of chemigenetic PKA biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

Depositor

Jin Zhang

Article

Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

Insert

HaloAKAR2.2

Tags

T2A-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCnRed

Plasmid

#232908

Purpose

Electroporation vector for expression of the lambda Red recombineering system in Cupriavidus necator.

Depositor

Anita Emmerstorfer-Augustin

Article

Arhar et al ACS Synth Biol. 2025 Feb 24. doi: 10.102

Insert

gam (gam Synthetic, phage Lambda)

Use

Synthetic Biology

Promoter

Pbad-rha

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pIVT-Flp-2A-BleoR

Plasmid

#232752

Purpose

IVT template for making bicistronic modRNA encoding Flippase and Zeocin resistance gene

Depositor

Kate Galloway

Article

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Inserts

FlpO
BleoR

Use

In vitro transcription

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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lenti-mAmetrine-CAAX

Plasmid

#216132

Purpose

Lentiviral vector for expressing mAmetrine localizing to the plasma membrane.

Depositor

Stefan Kubicek

Article

Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

Insert

EF1a-mAmetrine-CAAX-WPRE

Use

Lentiviral

Expression

Mammalian

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-opto-mMLKL

Plasmid

#208784

Purpose

Inducibly expresses mouse MLKL fused to light-activatable Cry2

Depositor

Petr Broz

Article

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

mCherry mMLKL Cry2 (Mlkl Mouse)

Use

Lentiviral

Tags

Cry2 and mCherry

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAG1188 mCherry-cagedFIREtag

Plasmid

#205177

Purpose

Expresses mCherry-cagedFIREtag in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

mCherry-FIREtag-DEVDG-MoA

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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TagBFP2-PIP4K2A

Plasmid

#202726

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mTagBFP2:PIP4K2A (PIP4K2A Human)

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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