We narrowed to 51,543 results for: des.1
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Plasmid#162796PurposeAll-in-one prime editor piggyBac transposonDepositorInsertSpCas9_ H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
UseCRISPR and Synthetic Biology; Piggybac transposonTagsSV40 NLSExpressionMammalianPromoterCAGAvailable SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCW-Cdt1ΔPIP-mCherry-Puro
Plasmid#193765PurposeDoxycyline-inducible (TetOn) human Cdt1ΔPIP (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)DepositorInsertCdt1ΔPIP-mCherry (CDT1 Human)
UseLentiviralTagsmCherryMutationPIP degron removed (a.a.1-19 removed)PromoterTREAvailable SinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delZn2
Plasmid#173935PurposeBacterial expression of PARP1 lacking Zn2 domainDepositorInsertPARP1delZn2 (PARP1 Human)
Tags6xHisExpressionBacterialMutationDeletion of residues 97-213 (V762A)Available SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONOR223 RpH-LAMP1-3xFLAG
Plasmid#163019Purposegateway donor vector encoding ratiometric sensor of lysosomal lumenal pHDepositorAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
MiniCoopR mitfa:BRAF
Plasmid#118846PurposeExpresses human BRAF V600E mutant and zebrafish mitfa specifically in zebrafish melanocytesDepositorAvailable SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-ART
Plasmid#174810PurposeBacterial expression of isolated PARP1 ART subdomainDepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-BRCT
Plasmid#174807PurposeBacterial expression of isolated PARP1 BRCT domainDepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-WGR
Plasmid#174808PurposeBacterial expression of isolated PARP1 WGR domainDepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
lenti-EGFR-L858R-T790M-dual-epegRNA
Plasmid#214100PurposeLentiviral vector expressing epegRNAs for the induction of EGFR L858R and T790M mutations, through tandem U6 expression of the two epegRNAs using independent U6 promoters.DepositorInsertEGFR L858R epegRNA/EGFR T790M epegRNA
UseLentiviralExpressionMammalianPromoterhU6Available SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
SGyA
Plasmid#160292PurposeDrives expression of Cas9 and a TdTomato fluorescent markerDepositorInsertsVas promoter
hSpCas9
tdTomato
CTCF insulator
Gypsy insulator
Left homology arm Y chromosome
Right homology arm Y chromosome
TagsT2A GFPExpressionInsectAvailable SinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
R2pH-LAMPx3ALFA
Plasmid#225136PurposeR2pH-LAMP1-labelled lysosomes with 3xLFADepositorInsertLamp1 (Lamp1 Mouse)
ExpressionMammalianAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
Opto-M1R
Plasmid#106069PurposeExpression of a chimeric putative G-protein coupled receptor (Rhodopsin-M1R; Opto-M1R; F11; 722)DepositorInsertOpto-M1R (CHRM1 Human, Bovine)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCHMP2A-NS3-blue
Plasmid#223532PurposeMammalian expression of CHMP2A fused to the hepatitis C virus protease NS3 through a short linker containing the NS3 cleavage site, along with a blue fluorescent protein.DepositorInsertCHMP2A (CHMP2A Human)
TagsNS3 Hepatitis C protease and mTurquoiseExpressionMammalianPromoterCMVAvailable SinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHMP2A-NS3-Green
Plasmid#223440PurposeMammalian expression of CHMP2A fused to the hepatitis C virus protease NS3 through a short linker containing the NS3 cleavage site, along with a green fluorescent protein.DepositorInsertCHMP2A (CHMP2A Human)
TagsNS3 Hepatitis C protease and mNeonGreenExpressionMammalianPromoterCMVAvailable SinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pICSL11060
Plasmid#68264PurposeLevel 1 Golden Gate Cassette: Cas9 expression cassette for plants (exemplified in Brassica oleracea)DepositorInsertPromoter/5UTR: CsVMV+ CDS:SpCas9 + 3UTR/terminator:35s
UseSynthetic BiologyExpressionPlantAvailable SinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFPN1-N-gamma-tubulin (334-449)
Plasmid#87859PurposeFluorescent fragment of gamma-tubulin containing gamma-tubulin DNA binding domain and NLSDepositorInsertgamma-tubulin 1 (TUBG1 Human)
TagsGFPExpressionMammalianMutationfragment from aa 334 to 449Available SinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR146
Plasmid#106047PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR146; Opto-GPR146; E1)DepositorInsertOpto-GPR146 (GPR146 Human, Bovine)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-A2AR
Plasmid#106066PurposeExpression of a chimeric putative G-protein coupled receptor (Rhodopsin-A2AR; Opto-A2AR; F8; 719)DepositorInsertOpto-A2AR (ADORA2A Human, Bovine)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delCAT
Plasmid#173942PurposeBacterial expression of PARP1 lacking CAT domainDepositorInsertPARP1delCAT (PARP1 Human)
Tags6xHisExpressionBacterialMutationDeletion of residues 661-1014Available SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMXs-WT-ATPIF1
Plasmid#85403PurposeRetroviral vector expressing wild type human ATPIF1DepositorAvailable SinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only