224,033 results

1. pcDNA3.1-hLbCpf1(TYCV) (pY230)

   Plasmid

   #89355

   Purpose

   Expresses humanized LbCpf1 variant that recognizes TYCV PAMs

   Depositor

   Feng Zhang

   Article

   Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

   Insert

   Cpf1, RR variant
   

   Use

   CRISPR

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   G532R/K595R

   Promoter

   CMV

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. TXNRD1

   Plasmid

   #38863

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   TXNRD1 (Txnrd1 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. TXNRD1

   Plasmid

   #38863

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   TXNRD1 (Txnrd1 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHFS:SEP-rGluA2 TKIT donor

   Plasmid

   #169440

   Purpose

   Donor DNA for TKIT knockin of SEP to GluA2 in rat

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Insert

   Super ecliptic pHluorin (SEP)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pROS10

   Plasmid

   #107924

   Purpose

   URA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

   Depositor

   Jean-Marc Daran

   Article

   Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

   Insert

   gRNA-CAN1.Y, gRNA-ADE2.Y
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLIK-Neo

   Plasmid

   #25735

   Purpose

   3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Neomycin resistance gene coexpression.

   Depositor

   Iain Fraser

   Article

   Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLIK-Neo

   Plasmid

   #25735

   Purpose

   3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Neomycin resistance gene coexpression.

   Depositor

   Iain Fraser

   Article

   Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBHM2329

   Plasmid

   #171686

   Purpose

   pRS316-PCnU6-scaffold, contains template for amplification of PCnU6 and scaffold for fusion PCR

   Depositor

   Hiten Madhani

   Article

   Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

   Insert

   PCnU6-sgRNA_scaffold
   

   Use

   CRISPR

   Expression

   Bacterial and Yeast

   Promoter

   C. neoformans U6

   Available Since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTRIPZ-hDDX5/17

   Plasmid

   #71307

   Purpose

   Inducible shRNA knockdown of both human DDX5 and DDX17 genes

   Depositor

   Allan Brasier

   Article

   Yang et al Mol Cell Proteomics. 2015 Oct;14(10):270

   Insert

   DDX5/17 shRNA (DDX17 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   TetO

   Available Since

   Dec. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTRIPZ-hDDX5/17

    Plasmid

    #71307

    Purpose

    Inducible shRNA knockdown of both human DDX5 and DDX17 genes

    Depositor

    Allan Brasier

    Article

    Yang et al Mol Cell Proteomics. 2015 Oct;14(10):270

    Insert

    DDX5/17 shRNA (DDX17 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    TetO

    Available Since

    Dec. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-HA-VprBP

    Plasmid

    #133586

    Purpose

    Expresses human VprBP wildtype in mammalian cells

    Depositor

    Yue Xiong

    Article

    Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

    Insert

    Vpr (HIV-1) binding protein (DCAF1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Wildtype human VprBP (Dcaf1)

    Available Since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. W118-1_hZBTB7B

    Plasmid

    #180380

    Purpose

    lentiviral transduction of human ZBTB7B gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

    Insert

    ZBTB7B (ZBTB7B Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHSN6A01

    Plasmid

    #50586

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    dCas9-VP64
    gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Tags

    3xFLAG, HA, and NLS

    Mutation

    dCas9 = defective in DNA cleavage; 4×minimal VP16…

    Promoter

    2×35Sp and AtU6-26p

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHSN6A01

    Plasmid

    #50586

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    dCas9-VP64
    gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Tags

    3xFLAG, HA, and NLS

    Mutation

    dCas9 = defective in DNA cleavage; 4×minimal VP16…

    Promoter

    2×35Sp and AtU6-26p

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pACAGW-oChiEFtdTomato-AAV

    Plasmid

    #32846

    Purpose

    AAV expression of mammalian codon optimized ChIEF for optogenetic excitation.

    Depositor

    Rory McQuiston

    Article

    Jurgens et al PLoS One. 2012;7(9):e45717. doi: 10.1371

    Insert

    oChiEF
    

    Use

    AAV

    Tags

    tdTomato

    Promoter

    CAGG

    Available Since

    March 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pACAGW-oChiEFtdTomato-AAV

    Plasmid

    #32846

    Purpose

    AAV expression of mammalian codon optimized ChIEF for optogenetic excitation.

    Depositor

    Rory McQuiston

    Article

    Jurgens et al PLoS One. 2012;7(9):e45717. doi: 10.1371

    Insert

    oChiEF
    

    Use

    AAV

    Tags

    tdTomato

    Promoter

    CAGG

    Available Since

    March 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

    Plasmid

    #139987

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. tetO-SOX9

    Plasmid

    #170703

    Purpose

    doxycycline-inducible overexpression of SOX9

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    SOX9 (SOX9 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet-ON

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. CoV2-Spike-D614G

    Plasmid

    #177960

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )
    

    Expression

    Mammalian

    Mutation

    D614G

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBAMD1-2

    Plasmid

    #61564

    Purpose

    Standardized mini-Tn5 delivery plasmid, kanamycin resistant

    Depositor

    Víctor de Lorenzo

    Article

    Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

    Type

    Empty backbone

    Use

    Synthetic Biology; Mini-tn5 vector

    Available Since

    Jan. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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