We narrowed to 1,472 results for: phr

1. pSLQ1852-3 pHR: U6-SpsgCD95-2 CMV-EGFP

   Plasmid

   #84252

   Purpose

   Expresses Sp sgCD95-2 gRNA with an EGFP fluorescent marker

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Insert

   Sp sgCD95-2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available Since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR-SIN-GFP-PTEN-Y16C

   Plasmid

   #30542

   Depositor

   Todd Waldman

   Article

   Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

   Insert

   GFP-PTEN-Y16C (PTEN Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   GFP-PTEN-Y16C

   Promoter

   Available Since

   Aug. 11, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pHR-SFFV-KRAB-dCas9-P2A-mCherry

   Plasmid

   #60954

   Purpose

   2nd Generation Lentiviral vector. Expresses an N-terminal KRAB-dCas9 fusion protein and mCherry

   Depositor

   Jonathan Weissman

   Article

   Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

   Insert

   KRAB-dCas9-P2A-mCherry fusion
   

   Use

   CRISPR and Lentiviral

   Tags

   2x NLS, HA, KRAB domain, and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available Since

   Jan. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR myc-MBP-SspB-iRFP-NLS

   Plasmid

   #223286

   Purpose

   An SspB-tagged maltose binding protein (MBP) with an infrared fluorescent protein for visualization. Used to demonstrate condensate dissolution using the OptoMBP system.

   Depositor

   Jared Toettcher

   Article

   Brumbaugh-Reed et al bioRxiv 2024.01.16.575860

   Insert

   Myc-MBP-SspB-irFP-NLS
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-CMV-TetO2_3C-Avi-His6__IRES-EmGFP

   Plasmid

   #113888

   Purpose

   Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic EmGFP

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Avi-His6

   Expression

   Mutation

   Promoter

   CMV-MIE-TetO2

   Available Since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. M23: pHR-SFFVp-FUSN::mCherry::SspB

   Plasmid

   #122148

   Purpose

   N terminal domain of FUS (1-214) tagged with SspB and mCherry.

   Depositor

   Clifford Brangwynne

   Article

   Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

   Insert

   FUS (FUS Human)
   

   Use

   Lentiviral

   Tags

   mCherry-sspB

   Expression

   Mammalian

   Mutation

   Contains only amino acids 1-214

   Promoter

   SFFV

   Available Since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-scFv-GCN4-sfGFP-GB1-dWPRE

   Plasmid

   #60907

   Purpose

   The plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP for imaging

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

   Insert

   scFv-GCN4
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. M43: pHR-SFFVp-iLID::EGFP::FTH1

   Plasmid

   #122149

   Purpose

   Self-assebled 24-mer tagged with iLID, and EGFP. Upon blue-light activation can bind up to 24 SspB-modified proteins.

   Depositor

   Clifford Brangwynne

   Article

   Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

   Insert

   FTH1 (FTH1 Human)
   

   Use

   Lentiviral

   Tags

   NLS-iLID-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available Since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLQ5428_pHR_EF1a-mCherry-P2A-Rfx_Cas13d-2xNLS-3xFLAG

   Plasmid

   #155305

   Purpose

   Lentiviral vector encoding Rfx Cas13d fused with 2xNLS, 3xFLAG, and 2A-tagged mCherry.

   Depositor

   Stanley Qi

   Article

   Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

   Insert

   mCherry 2A-tagged to Ruminococcus flavefaciens XPD3002 Cas13d
   

   Use

   Lentiviral

   Tags

   2xNLS-3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR hEF1A-BFP-P2A-rtTA-WPRE

    Plasmid

    #223288

    Purpose

    A lentiviral vector expressing BFP and the TetON transcription factor rtTA.

    Depositor

    Jared Toettcher

    Article

    Brumbaugh-Reed et al bioRxiv 2024.01.16.575860

    Insert

    BFP-P2A-rtTA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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