We narrowed to 31,018 results for: ica

1. pRSFDuet-3C-AcrIC8

   Plasmid

   #207410

   Purpose

   Protein purification vector to produce AcrIC8 in E. coli

   Depositor

   Yan Zhang

   Article

   Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

   Insert

   T7-HRV3C-AcrIC8
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1(-)-Green Falcan1

   Plasmid

   #199705

   Purpose

   green fluorescent indicator for cAMP imaging

   Depositor

   Tetsuya Kitaguchi

   Article

   Hiasa et al RSC Adv. 2023 May 22;13(23):15514-15520.

   Insert

   Green Falcan1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBad-HaloGFP-Ca0.5

   Plasmid

   #198632

   Purpose

   Green fluorescent Ca2+ ion indicator for bacterial expression.

   Depositor

   Robert Campbell

   Article

   Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

   Insert

   HaloGFP-Ca0.5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD promoter

   Available since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBad-HaloGFP-Ca0.2

   Plasmid

   #198631

   Purpose

   Green fluorescent Ca2+ ion indicator for bacterial expression.

   Depositor

   Robert Campbell

   Article

   Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

   Insert

   HaloGFP-Ca0.2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD promoter

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBad-HaloGFP-Ca1

   Plasmid

   #198633

   Purpose

   Green fluorescent Ca2+ ion indicator for bacterial expression.

   Depositor

   Robert Campbell

   Article

   Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

   Insert

   HaloGFP-Ca1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD promoter

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBR322-Pdp1_aa1-133-HiBiT

   Plasmid

   #198313

   Purpose

   Loads a truncated payload; for identification of Pdp1 packaging domain

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   Pdp1_aa1-133-HiBiT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBR322-Pdp1_aa1-289-HiBiT

   Plasmid

   #198311

   Purpose

   Loads a truncated payload; for identification of Pdp1 packaging domain

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   Pdp1_aa1-289-HiBiT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBR322-Pdp1_aa1-190-HiBiT

   Plasmid

   #198312

   Purpose

   Loads a truncated payload; for identification of Pdp1 packaging domain

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   Pdp1_aa1-190-HiBiT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAc5.1B-lambdaN-HA-DmGIGYF_1-640_AH

   Plasmid

   #148933

   Purpose

   Insect Expression of DmGIGYF1_1-640

   Depositor

   Elisa Izaurralde

   Article

   Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

   Insert

   DmGIGYF1_1-640 (Gyf Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAc5.1B-lambdaN-HA-DmGIGYF-Y58AY60AM65A_AH

    Plasmid

    #148940

    Purpose

    Insect Expression of DmGIGYF1-Y58AY60AM65A

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF1-Y58AY60AM65A (Gyf Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. hp-66%GC-40bp

    Plasmid

    #169200

    Purpose

    Force-activating hairpin for 3'-to-5' helicase

    Depositor

    Tom Perkins

    Article

    Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

    Insert

    66%GC-40bp-hairpin
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. hp-75%GC-20bp

    Plasmid

    #169201

    Purpose

    Force-activating hairpin for 3'-to-5' helicase

    Depositor

    Tom Perkins

    Article

    Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

    Insert

    75%GC-20bp-hairpin
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. hp-75%GC-40bp

    Plasmid

    #169202

    Purpose

    Force-activating hairpin for 3'-to-5' helicase

    Depositor

    Tom Perkins

    Article

    Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

    Insert

    75%GC-40bp-hairpin
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. hp-100%GC-20bp

    Plasmid

    #169203

    Purpose

    Force-activating hairpin for 3'-to-5' helicase

    Depositor

    Tom Perkins

    Article

    Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

    Insert

    100%GC-20bp-hairpin
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. hp-50%GC-Left-half-120bp

    Plasmid

    #169204

    Purpose

    Force-activating hairpin for 3'-to-5' helicase

    Depositor

    Tom Perkins

    Article

    Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

    Insert

    50%GC-Left-half-120bp-hairpin
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. hp-50%GC-Right-half-120bp

    Plasmid

    #169205

    Purpose

    Force-activating hairpin for 3'-to-5' helicase

    Depositor

    Tom Perkins

    Article

    Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

    Insert

    50%GC-Right-half-120bp-hairpin
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. hp-50-0-50%GC-Left-half-120bp

    Plasmid

    #169206

    Purpose

    Force-activating hairpin for 3'-to-5' helicase

    Depositor

    Tom Perkins

    Article

    Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

    Insert

    50-0-50%GC-Left-half-120bp-hairpin
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. hp-50%GC-20bp

    Plasmid

    #169196

    Purpose

    Force-activating hairpin for 3'-to-5' helicase

    Depositor

    Tom Perkins

    Article

    Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

    Insert

    50%GC-20bp-hairpin
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. hp-50%GC-40bp

    Plasmid

    #169197

    Purpose

    Force-activating hairpin for 3'-to-5' helicase

    Depositor

    Tom Perkins

    Article

    Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

    Insert

    50%GC-40bp-hairpin
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. hp-50%GC-40bp-Revbindsite

    Plasmid

    #169198

    Purpose

    Force-activating hairpin for 5'-to-3' helicase

    Depositor

    Tom Perkins

    Article

    Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

    Insert

    50%GC-40bp-hairpin-binding-site-reversed
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. hp-66%GC-20bp

    Plasmid

    #169199

    Purpose

    Force-activating hairpin for 3'-to-5' helicase

    Depositor

    Tom Perkins

    Article

    Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

    Insert

    66%GC-20bp-hairpin
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pNUT2

    Plasmid

    #175745

    Purpose

    Single-component photo-inducible (LOV2-based) nucleolus-targeting tool 2

    Depositor

    Yubin Zhou

    Article

    Tan et al Nucleic Acids Res. 2022 Mar 23. pii: 655

    Insert

    mCherry-LOV2-NoLS (human nuclear factor-κB inducing kinase)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Lyn-CFP-FRB1C_no-linker

    Plasmid

    #162434

    Purpose

    for use in CIT (chemically induced trimerization) system

    Depositor

    Takanari Inoue

    Article

    Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

    Insert

    Lyn-CFP-FRB1C_no_linker
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Lyn-YFP-Clover-FRB1N

    Plasmid

    #162439

    Purpose

    for use in CIT (chemically induced trimerization) system

    Depositor

    Takanari Inoue

    Article

    Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

    Insert

    Lyn-YFP-Clover-FRB1N
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET-11a (D3 - L39V)

    Plasmid

    #136607

    Purpose

    Expresses DANCER-3 mutant (reversion L39V)

    Depositor

    Roberto Chica

    Article

    Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

    Insert

    DANCER-3 mutant (L39V reversion)
    

    Use

    Tags

    5x-Histag

    Expression

    Bacterial

    Mutation

    Mutation from GB1 WT: Y3F-L7I-A34F-V54I

    Promoter

    T7 promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJ414 - DANCER-2

    Plasmid

    #136386

    Purpose

    Expresses DANCER-2 dynamic protein

    Depositor

    Roberto Chica

    Article

    Davey et al Nat Chem Biol. 2017 Dec;13(12):1280-1285

    Insert

    DANCER-2
    

    Use

    Tags

    6x-His tag (N-term of insert)

    Expression

    Bacterial

    Mutation

    Mutation from GB1 WT: Y3F-L5A-L7I-A34F-V39L

    Promoter

    T7 promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJ414 - NERD-S

    Plasmid

    #136388

    Purpose

    Expresses NERD-S static protein

    Depositor

    Roberto Chica

    Article

    Davey et al Nat Chem Biol. 2017 Dec;13(12):1280-1285

    Insert

    NERD-S
    

    Use

    Tags

    6x-His tag (N-term of insert)

    Expression

    Bacterial

    Mutation

    Mutation from GB1 WT: Y3F-L7I-A34F-V39I-V54I

    Promoter

    T7 promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJ414 - NERD-C

    Plasmid

    #136389

    Purpose

    Expresses NERD-C static protein

    Depositor

    Roberto Chica

    Article

    Davey et al Nat Chem Biol. 2017 Dec;13(12):1280-1285

    Insert

    NERD-C
    

    Use

    Tags

    6x-His tag (N-term of insert)

    Expression

    Bacterial

    Mutation

    Mutation from GB1 WT: Y3F-L7I-F30L-V39I

    Promoter

    T7 promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET-11a (D3 - I54V)

    Plasmid

    #136608

    Purpose

    Expresses DANCER-3 mutant (reversion I54V)

    Depositor

    Roberto Chica

    Article

    Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

    Insert

    DANCER-3 mutant (I54V reversion)
    

    Use

    Tags

    6x-Histag

    Expression

    Bacterial

    Mutation

    Mutation from GB1 WT: Y3F-L7I-A34F-V39L

    Promoter

    T7 promoter

    Available since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET-11a (D3 - F34A)

    Plasmid

    #136606

    Purpose

    Expresses DANCER-3 mutant (reversion F34A)

    Depositor

    Roberto Chica

    Article

    Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

    Insert

    DANCER-3 mutant (F34A reversion)
    

    Use

    Tags

    6x-Histag

    Expression

    Bacterial

    Mutation

    Mutation from GB1 WT: Y3F-L7I-V39L-V54I

    Promoter

    T7 promoter

    Available since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET-11a (D3 - I7L)

    Plasmid

    #136605

    Purpose

    Expresses DANCER-3 mutant (reversion I7L)

    Depositor

    Roberto Chica

    Article

    Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

    Insert

    DANCER-3 mutant (I7L reversion)
    

    Use

    Tags

    6x-Histag

    Expression

    Bacterial

    Mutation

    Mutation from GB1 WT: Y3F-A34F-V39L-V54I

    Promoter

    T7 promoter

    Available since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET-11a (D3 - F3Y)

    Plasmid

    #136604

    Purpose

    Expresses DANCER-3 mutant (reversion F3Y)

    Depositor

    Roberto Chica

    Article

    Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

    Insert

    DANCER-3 mutant (F3Y reversion)
    

    Use

    Tags

    6x-Histag

    Expression

    Bacterial

    Mutation

    Mutation from GB1 WT: L7I-A34F-V39L-V54I

    Promoter

    T7 promoter

    Available since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAV0517

    Plasmid

    #133492

    Purpose

    Expression of nucleo-cytoplasmic GFP in P-cells

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pHis5(StuI)-p(map3)-GFP-terminator(nmt)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAV0772

    Plasmid

    #133516

    Purpose

    Expression of sfGFP tagged beta-tubulin to monitor microtubules

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pAde6(PmeI)-p(atb2)-sfGFP-atb2-terminator(atb2)-hphMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAV0607

    Plasmid

    #133504

    Purpose

    Expression of mCherry targeted to the plasma membrane with an amphipathic helix of RitC

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pAde6(PmeI)-p(act1)-mCherry-RitC-terminator(ScADH1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAV0763

    Plasmid

    #133497

    Purpose

    Expression of nucleo-cytoplasmic mTagBFP2 in M-cells

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pAde6(PmeI)-p(mam1*)-mTagBFP2-terminator(ScADH1)-kanMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCfB4779

    Plasmid

    #106145

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_2, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCfB4784

    Plasmid

    #106148

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntF_2, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCfB4785

    Plasmid

    #106149

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntF_3, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCfB4783

    Plasmid

    #106146

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_3, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCfB4781

    Plasmid

    #106140

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntC_1, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCfB4788

    Plasmid

    #106139

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntA_1, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pTorPE-Y-GECO2m

    Plasmid

    #105066

    Purpose

    A yellow fluorescent Ca2+ indicator variant with medium binding affinity and kinetics for expression in bacterial cells

    Depositor

    Robert Campbell

    Article

    Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

    Insert

    Y-GECO2m
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. p3E-GFP-HA no-pA

    Plasmid

    #82589

    Purpose

    3' entry vector containing HA epitope fused to C-terminus of GFP without pA for imaging, epitope labeling/purification

    Depositor

    Kryn Stankunas

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    GFP-HA (no pA)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. BS.YxiN.Twin1.a207-368.wt

    Plasmid

    #13531

    Depositor

    David McKay

    Article

    Caruthers et al Acta Crystallograph Sect F Struct Biol C

    Insert

    B. subtilis DEAD-box helicase YxiN second domain
    

    Use

    Tags

    intein-chitin binding domain

    Expression

    Bacterial

    Mutation

    deleted amino acid residues 1-206 and 369-479

    Promoter

    Available since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pTorPE-Y-GECO2s

    Plasmid

    #105067

    Purpose

    A yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cells

    Depositor

    Robert Campbell

    Article

    Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

    Insert

    Y-GECO2s
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pTorPE-Y-GECO2.1s

    Plasmid

    #105068

    Purpose

    A yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cells

    Depositor

    Robert Campbell

    Article

    Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

    Insert

    Y-GECO2.1s
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pLenti_dCas9-KRAB-MeCP2_Blast

    Plasmid

    #221501

    Purpose

    Lentiviral construct for expression of the dCas9-KRAB-MeCP2 CRISPRi vector

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    dCas9-KRAB-MeCP2-T2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CMV

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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49. pdCas9-bacteria

    Plasmid

    #44249

    Purpose

    aTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    dCas9 (bacteria)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    pLtetO-1

    Available since

    April 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. T7-VEE-IRES-Puro

    Plasmid

    #58970

    Purpose

    noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

    Depositor

    Steven Dowdy

    Article

    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

    Type

    Empty backbone

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    26S subgenomic promoter

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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