We narrowed to 178,163 results for: Des

1. pNQL006-NANOG-FKBPV-HA2-P2A-eGFP targeting construct

   Plasmid

   #175554

   Purpose

   Targeting vector to introduce an FKBPV-HA2-P2A-eGFP cassette at the mouse NANOG locus. Degradation Tag (dTAG) system.

   Depositor

   Elzo de Wit

   Article

   Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

   Insert

   NANOG
   

   Use

   Mouse Targeting

   Tags

   FKBPV-HA2-P2A-eGFP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLPC TRF2 deltaB deltaM

   Plasmid

   #18008

   Depositor

   Titia de Lange

   Article

   Karlseder et al Science. 2002 Mar 29. 295(5564):2446-9.

   Insert

   hTRF2 (TERF2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Deleted Basic Domain (aa1-44) and Myb Domain (aa4…

   Promoter

   Available since

   May 29, 2008

   Availability

   Academic Institutions and Nonprofits only

3. VPS13D^EGFP

   Plasmid

   #174109

   Purpose

   The insert is a codon optimized DNA sequence encoding VPS13D with an internal EGFP.

   Depositor

   Pietro De Camilli

   Article

   Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

   Insert

   Codon optimized VPS13D
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   With an internal EGFP tag in aminoacid position 1…

   Promoter

   CMV

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBAMD1-4

   Plasmid

   #61565

   Purpose

   Standardized mini-Tn5 delivery plasmid, streptomycin/spectinomycin resistant

   Depositor

   Víctor de Lorenzo

   Article

   Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

   Type

   Empty backbone

   Use

   Synthetic Biology; Mini-tn5 vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. iRFP-PH-PLCdelta1

   Plasmid

   #66841

   Purpose

   expression of near-infrared PI(4,5)P2 biosensor

   Depositor

   Pietro De Camilli

   Article

   Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

   Insert

   PH-PLCdelta1
   

   Use

   Tags

   iRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLENTI_hACE2_PURO

   Plasmid

   #155295

   Purpose

   Lentiviral vector to generate hACE2 stable expressing cell line

   Depositor

   Raffaele De Francesco

   Insert

   hACE2 (ACE2 Human)
   

   Use

   Lentiviral

   Tags

   C9

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pEGFPN1-human Dynamin 1aa K44A

   Plasmid

   #22197

   Depositor

   Pietro De Camilli

   Article

   Lee et al Proc Natl Acad Sci U S A. 2002 Jan 8. 99

   Insert

   human Dynamin 1aa K44A (DNM1 Human)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   K44A

   Promoter

   Available since

   Nov. 2, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. VPS13A^Halo

   Plasmid

   #118759

   Purpose

   VPS13A internally tagged with Halo at residue 1372.

   Depositor

   Pietro De Camilli

   Article

   Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

   Insert

   VPS13A (VPS13A Human)
   

   Use

   Tags

   Halo tag inserted at position 1372

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. SETD2-GFP

   Plasmid

   #80653

   Purpose

   mammalian expression of SETD2-GFP

   Depositor

   Sérgio De Almeida

   Article

   Carvalho et al Elife. 2014 May 6;3:e02482. doi: 10.7554

   Insert

   SETD2 (SETD2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEGFPN1-human Dynamin 1aa

    Plasmid

    #22163

    Depositor

    Pietro De Camilli

    Article

    Lee et al Proc Natl Acad Sci U S A. 2002 Jan 8. 99

    Insert

    human Dynamin 1aa (DNM1 Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    D744N

    Promoter

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBAMD1-6

    Plasmid

    #61566

    Purpose

    Standardized mini-Tn5 delivery plasmid, gentamicin resistant

    Depositor

    Víctor de Lorenzo

    Article

    Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

    Type

    Empty backbone

    Use

    Synthetic Biology; Mini-tn5 vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBS Zpapc

    Plasmid

    #25774

    Depositor

    Edward De Robertis

    Article

    Yamamoto et al Development. 1998 Sep . 125(17):3389-97.

    Insert

    Papc (pcdh8 Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pCS2 GFP-GSK3-MAPK

    Plasmid

    #29689

    Purpose

    GFP reporter protein fused to three GSK3 phosphorylation sites

    Depositor

    Edward De Robertis

    Article

    Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

    Insert

    GFP glycogen synthase kinase 3 recognition site primed by MAPK
    

    Use

    Tags

    Flag and Flag-EGFP

    Expression

    Mammalian

    Mutation

    recognition sites only, and E3 ligase site

    Promoter

    SP6

    Available since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCS2 GFP-GSK3mut-MAPK

    Plasmid

    #29690

    Purpose

    GFP reporter protein fused to three mutated GSK3 phosphorylation sites

    Depositor

    Edward De Robertis

    Article

    Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

    Insert

    GFP glycogen synthase kinase 3 recognition site mutated primed by MAPK
    

    Use

    Tags

    Flag and Flag-EGFP

    Expression

    Mammalian

    Mutation

    recognition sites only, and E3 ligase site, GSK3 …

    Promoter

    SP6

    Available since

    Jan. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. eMagAF-EGFP-PM

    Plasmid

    #162247

    Purpose

    Blue-light dependent heterodimerization system

    Depositor

    Pietro De Camilli

    Article

    Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

    Insert

    eMagAF-EGFP-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LV-LIN28A

    Plasmid

    #117057

    Purpose

    Overexpression of mouse LIN28A

    Depositor

    Michele De Palma

    Article

    Baer et al Nat Cell Biol. 2016 Jul;18(7):790-802. d

    Insert

    LIN28A (Lin28a Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hPGK promoter

    Available since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pVDL9.3

    Plasmid

    #168299

    Purpose

    Secretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyA

    Depositor

    Víctor de Lorenzo

    Article

    Tzschaschel et al Nat Biotechnol. 1996 Jun;14(6):765-9. do

    Insert

    Segment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    LacI-Plac

    Available since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLPC-NFLAG TRF1

    Plasmid

    #16058

    Depositor

    Titia de Lange

    Article

    Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

    Insert

    hTRF1 (TERF1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mutation

    aa2-439

    Promoter

    Available since

    Nov. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

19. mCherry-CRY2-iSH2

    Plasmid

    #66839

    Purpose

    Expression of a light-inducible PI3-kinase, generated by fusing the iSH2 domain of human p85α (residues 420–615) to mCherry-CRY2. Binds endogenous PI3-kinase p110α catalytic subunits constitutively.

    Depositor

    Pietro De Camilli

    Article

    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

    Insert

    iSH2
    

    Use

    Tags

    mCherry-CRY2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mCherry- eMagBF - 5ptaseOCRL

    Plasmid

    #162254

    Purpose

    Blue-light dependent heterodimerization system

    Depositor

    Pietro De Camilli

    Article

    Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

    Insert

    mCherry- eMagBF - 5ptaseOCRL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge