We narrowed to 3,113 results for: GAI

1. pFBOH-MHL.EED

   Plasmid

   #125174

   Purpose

   Expresses human EED in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.

   Depositor

   Chen Davidovich

   Article

   Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

   Insert

   Polycomb protein EED (EED Human)
   

   Use

   Tags

   His-TEV

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   May 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. px458-CCND1

   Plasmid

   #172630

   Purpose

   Expresses a gRNA against CCND1 and Cas9 from S. pyogenes with 2A-EGFP

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   gRNA against CCND1 (CCND1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. shERK2b-mlpx-puro

   Plasmid

   #65230

   Purpose

   encodes a shRNA against ERK2

   Depositor

   Gerardo Ferbeyre

   Article

   Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

   Insert

   shRNA against ERK2 (MAPK1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Aug. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. JDW 836 (pCS2-V5-mScarlet-I-hsKRAS4A-WT)

   Plasmid

   #156411

   Purpose

   pCS2 CMV expression vector driving V5-tagged mScarlet-I-fused human KRAS4A

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   human wild type KRAS4A-WT (KRAS Human)
   

   Use

   Tags

   mScarlet-I

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. shERK2a-mlpx-puro

   Plasmid

   #65229

   Purpose

   encodes a shRNA against ERK2

   Depositor

   Gerardo Ferbeyre

   Article

   Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

   Insert

   shRNA against ERK2 (MAPK1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFBOH-MHL.EZH2

   Plasmid

   #125171

   Purpose

   Expresses human EZH2 in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.

   Depositor

   Chen Davidovich

   Article

   Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

   Insert

   EZH2 (EZH2 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   May 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. JDW 763 (pCS2-EYFP-hsKRAS4B-WT)

   Plasmid

   #156408

   Purpose

   pCS2 CMV expression vector driving EYFP fused human KRAS4B

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   human wild type KRAS4B-WT (KRAS Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. shERK1a-mlpx-puro

   Plasmid

   #65228

   Purpose

   encodes a shRNA against ERK1

   Depositor

   Gerardo Ferbeyre

   Article

   Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

   Insert

   shRNA against ERK2 (MAPK1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pENTR1A-Yap S127A

   Plasmid

   #46050

   Depositor

   Xin Chen

   Article

   Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

   Insert

   Yes-kinase associated protein (YAP1 Human)
   

   Use

   Entry vector

   Tags

   2X Flag tag

   Expression

   Mutation

   S127A

   Promoter

   Available since

   July 2, 2013

   Availability

   Academic Institutions and Nonprofits only

10. PX458-sgEIF4EBP1

    Plasmid

    #128097

    Purpose

    CRISPR gRNA against human EIF4EBP1 (4EBP1) with Cas9 from S. pyogenes and 2A-EGFP

    Depositor

    Chi Van Dang

    Article

    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

    Insert

    CRISPR sgRNA against human 4EBP1 (EIF4EBP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBRPy CAGGS-Mbd3-IRES-PURO

    Plasmid

    #52376

    Purpose

    Mammalian expression vector for constitutive expression of Mbd3

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    Mbd3 (Mbd3 Mouse)
    

    Use

    Tags

    2xFlag (DYKDDK)

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. shERK2b-mlpx-neo

    Plasmid

    #65231

    Purpose

    encodes a shRNA against ERK2

    Depositor

    Gerardo Ferbeyre

    Article

    Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

    Insert

    shRNA against ERK2 (MAPK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Ikaros-CRISPR-Nick2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

    Plasmid

    #75243

    Purpose

    CRISPR/Cas9 NICKASE plasmid against human Ikaros (2/2)

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against human Ikaros (IKZF1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. JDW 822 (pDONR221-V5-mScarlet-I-KRAS4A-WT)

    Plasmid

    #156405

    Purpose

    Gateway middle entry clone encoding V5-tagged mScarlet-I-fused to human KRAS4A, includes a stop codon

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human wild type KRAS4A-WT (KRAS Human)
    

    Use

    Gateway cloning

    Tags

    mScarlet-I

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Ikaros-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

    Plasmid

    #75242

    Purpose

    CRISPR/Cas9 NICKASE plasmid against human Ikaros (1/2)

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against human Ikaros (IKZF1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGMC00030 (aka pRC0707)

    Plasmid

    #209673

    Purpose

    Lentiviral vector expressing PP7-P65-HSF1

    Depositor

    Raj Chari

    Article

    Yang et al Sci Adv. 2024 Feb 16;10(7):eadj2445. doi

    Insert

    PP7-P65-HSF1 (HSF1, RELA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. px458-AMBRA1 (optimized for N-terminal knock-in)

    Plasmid

    #172608

    Purpose

    Expresses a gRNA against AMBRA1 and Cas9 from S. pyogenes with 2A-EGFP. This gRNA is suitable for the N-terminal knock-in of AMBRA1.

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    gRNA against AMBRA1 (AMBRA1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLY095-EFS-ERBB2 HER2-Blast-WPRE

    Plasmid

    #192196

    Purpose

    Lenti-HER2-OE-Blast

    Depositor

    Sidi Chen

    Article

    Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

    Insert

    Lenti-HER2-OE-Blast (ERBB2 Synthetic)
    

    Use

    Lentiviral; Mammalian expression

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTwist-SARS-CoV-2 Δ18 B.1.617.2v1

    Plasmid

    #179905

    Purpose

    Encodes SARS-CoV-2 B.1.1.529 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2022 Jan 6. pii: S0092-8674(21)014

    Insert

    SARS-CoV-2 B.1.617.2v1 Spike truncated to remove 18 amino acids (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T19R; G142D; E156G; Δ157-158; L452R; T478K; D614G…

    Promoter

    CAG

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.3_SARS2_BQ.1.1

    Plasmid

    #194493

    Purpose

    expressing SARS-CoV-2 BQ.1.1 spike protein for pseudovirus production

    Depositor

    David Nemazee

    Article

    Zhou et al Immunity. 2023 Mar 14;56(3):669-686.e7.

    Insert

    Spike (BQ.1.1) (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R346T, K444T, N460K and c-terminal 18aa del on th…

    Promoter

    CMV

    Available since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge