We narrowed to 17,708 results for: URE

1. pFOSGE_COF3H09

   Plasmid

   #71003

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   SNF4Agamma (SNF4Agamma Fly)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pFOSGE_COF3C07

   Plasmid

   #70985

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   14-3-3zeta (14-3-3zeta Fly)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pFOSGE_COF2B09

   Plasmid

   #70918

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   gro (gro Fly)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pFOSGE_COF3C03

   Plasmid

   #70959

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   Taf4 (Taf4 Fly)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

5. VP12

   Plasmid

   #72247

   Purpose

   Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A and Q926A

   Promoter

   CMV

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. p6XHis_NLS-SaCas9

   Plasmid

   #101086

   Purpose

   Express SaCas9 in bacteria with a 6xHis tag for purification

   Depositor

   Rick Tarleton

   Article

   Soares Medeiros et al MBio. 2017 Nov 7;8(6). pii: e01788-17. d

   Insert

   CRISPR-associated protein Cas9/Csn1
   

   Use

   CRISPR

   Tags

   TrxA-6xHis-STag

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available Since

   Dec. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. (559) pAAV EF-1a KRAB-SadCas9 U6-gRNA

   Plasmid

   #163024

   Purpose

   Expression of CRISPRi with U6-gRNA (Bsa1 sites)

   Depositor

   Tonia Rex

   Article

   Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

   Insert

   Mammalian codon-optimized SadCas9
   

   Use

   AAV

   Tags

   KRAB, SV40 polyA, and myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   hEF-1a

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. BPK4410 - human expression plasmid for SpCas9 Cluster 1 (HypaCas9)

   Plasmid

   #101178

   Purpose

   Human expression plasmid for SpCas9 Cluster 1 variant (HypaCas9): CMV-T7-hSpCas9-Cluster1(N692A, M694A, Q695A, H698A)-NLS(SV40)-3xFLAG

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster1(N692A/M694A/Q695A/H698A)
   

   Use

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   N692A/M694A/Q695A/H698A

   Promoter

   CMV

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAnc80L65

   Plasmid

   #68837

   Purpose

   Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct. Note that an improved version is available: Addgene plasmid# 92307. See comments for details

   Depositor

   Luk Vandenberghe

   Article

   Zinn et al Cell Rep. 2015 Jul 28. pii: S2211-1247(1

   Insert

   Ancestral AAV Capsid Anc80L65
   

   Use

   AAV and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Rep2

   Available Since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX6P1-HA-MEK1

    Plasmid

    #53159

    Purpose

    Expresses GST-HA tagged human MEK1 from bacterial expression vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK1 (MAP2K1 Human)
    

    Use

    Tags

    GST and HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSECC

    Plasmid

    #60820

    Purpose

    3rd generation vector. Expresses a sgRNA of interest, Cas9 and Cre

    Depositor

    Tyler Jacks

    Article

    Sanchez-Rivera et al Nature. 2014 Oct 22. doi: 10.1038/nature

    Inserts

    Cas9
    Cre
    

    Use

    CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    BsmBI site eliminated by C->A; E308G

    Promoter

    EFS and EFS (after Cas9-2A)

    Available Since

    Nov. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX neo PFKL-GFP

    Plasmid

    #138289

    Purpose

    Mammalian Expression, Lentiviral. Expression of human phosphofructokinase, liver isoform PFKL-GFP fusion protein

    Depositor

    Gaudenz Danuser

    Article

    Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

    Insert

    PFKL (PFKL Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJSC114 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant

    Plasmid

    #101215

    Purpose

    Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant K848A/K1003A/R1060A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    K848A, K1003A and R1060A

    Promoter

    T7

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti CMV SNAP MDC1

    Plasmid

    #227375

    Purpose

    SNAP–MDC1 expression

    Depositor

    David Pellman

    Article

    Papathanasiou et al Nature. 2023 Jul;619(7968):184-192. doi:

    Insert

    MDC1, mediator of DNA damage checkpoint 1 (MDC1 Human)
    

    Use

    Lentiviral

    Tags

    SNAP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDEST-FRT/T0-GFP-BRCA1

    Plasmid

    #71116

    Purpose

    mammalian expression of GFP-BRCA1 siRNA resistant construct

    Depositor

    Daniel Durocher

    Article

    Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TO

    Available Since

    Dec. 9, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pLVX neo ACTN1-GFP

    Plasmid

    #138293

    Purpose

    Mammalian Expression, Lentiviral. Expression of human alpha-actinin-1-GFP fusion protein

    Depositor

    Gaudenz Danuser

    Article

    Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

    Insert

    ACTN1 (ACTN1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pINDUCER-21-SPI1

    Plasmid

    #97039

    Purpose

    Expresses SPI1 in mammalian cells

    Depositor

    George Daley

    Article

    Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

    Insert

    Spi-1 Proto-Oncogene (SPI1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet on

    Available Since

    Sept. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

18. (565) pAAV Alb-AAT KRAB-SadCas9 U6-gSTOP

    Plasmid

    #163030

    Purpose

    Expression of CRISPRi with gSTOP gRNA

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Mammalian codon-optimized SadCas9
    

    Use

    AAV

    Tags

    KRAB, SV40 pA, and myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Alb-AAT

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJSC173 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant

    Plasmid

    #101218

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant N692A/M694A/Q695A/H698A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    N692A, M694A, Q695A and H698A

    Promoter

    T7

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDEST-FRT/T0-Flag-BRCA1

    Plasmid

    #71117

    Purpose

    mammalian expression of Flag-BRCA1 siRNA resistant construct

    Depositor

    Daniel Durocher

    Article

    Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TO

    Available Since

    Nov. 12, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge