We narrowed to 14,318 results for: cas9

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pEN017-SpCas9-hiNLS-t-M4

Plasmid

#234455

Purpose

Expresses hiNLS variant t-M4 inside 0C triNLS-SpCas9-NGG

Depositor

Ross Wilson

Publication

Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

Insert

SpCas9 hiNLS t-M4

Use

CRISPR

Tags

His6, Nucleoplasmin, SV40, and c-Myc

Expression

Bacterial

Mutation

Cys at positions 80 and 574 have been mutated to …

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCas9_soc_crRNA

Plasmid

#257705

Purpose

Bacterial expression plasmid encoding Cas9 nuclease, tracrRNA, and crRNA targeting the soc gene of T4 phage

Depositor

Juhong Chen

Publication

He et al Anal Chem. 2024 Nov 12;96(45):18301-1831

Insert

SpCas9

Use

CRISPR

Expression

Bacterial

Available since

June 16, 2026

Availability

Academic Institutions and Nonprofits only

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pBBR_Cas9_sp1

Plasmid

#154851

Purpose

Expresses SpCas9 and sgRNA that targets the upp gene in Rhodobacter sphaeroides genome

Depositor

John van der Oost

Publication

Mougiakos et al Microb Cell Fact. 2019 Nov 25;18(1):204.

Inserts

Spcas9 gene
sgRNA scaffold

Use

CRISPR

Tags

6xHis

Mutation

Codon harmonized variant for expression in Rhodob…

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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AAVS1-idCas9-vpr

Plasmid

#89985

Purpose

inducible CRISPR-ON system for controllable gene activation; this plasmid is used to insert dCas9-VPR casette in one allele of AAVS1 locus

Depositor

Jie Na

Publication

Guo et al Protein Cell. 2017 Jan 23. doi: 10.1007/

Insert

dCas9-VPR

Expression

Mammalian

Promoter

TRE-tight

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pT7-PE7 for IVT

Plasmid

#214813

Purpose

Template for in vitro transcription of PE7

Depositor

Brittany Adamson

Publication

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7

Use

Template for in vitro transcription

Tags

SV40 bpNLS and c-Myc NLS

Promoter

T7 (inactivated)

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJSC052 - Bacterial expression plasmid for SpCas9, REC3 FRET variant

Plasmid

#101201

Purpose

Bacterial expression plasmid for SpCas9, REC3 FRET variant

Depositor

Jennifer Doudna, Keith Joung

Publication

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant C80S/C574S/S701C/S960C

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

C80S, C574S, S701C and S960C

Promoter

T7

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pJSC033 - Bacterial expression plasmid for SpCas9, REC2 FRET variant

Plasmid

#101200

Purpose

Bacterial expression plasmid for SpCas9, REC2 FRET variant

Depositor

Jennifer Doudna, Keith Joung

Publication

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant C80S/C574S/E60C/D273C

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

C80S, C574S, E60C and D273C

Promoter

T7

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pLentiV2-dCas9-VP64

Plasmid

#141104

Purpose

Expression of gRNA and dCas9-VP64 from the same plasmid for CRISPRa

Depositor

Igor Ulitsky

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET-Octo_VirD2_NLS-Cas9-6xHis

Plasmid

#232296

Purpose

Expresses Cas9 protein fused with octopine-type VirD2-originating NLS

Depositor

Hee-Sik Kim

Publication

Le et al Proc Natl Acad Sci U S A. 2025 Mar 11;12

Insert

Octo_VirD2_NLS-Cas9-6xHis

Use

CRISPR

Tags

6xHis and Octopine-type VirD2-derived NLS

Expression

Bacterial

Promoter

T7

Available since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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YN2_1_IL50_HOlocus

Plasmid

#194426

Purpose

Expresses Cas9 and sgRNA cassettes for targeting the HO locus in yeast Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Publication

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Insert

Cas9

Expression

Yeast

Mutation

NA

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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YN2_1_LT84_RPL13A

Plasmid

#177712

Purpose

Expresses Cas9 and sgRNA cassette for targeting RPL13A in yeast Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Publication

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Insert

Cas9

Use

CRISPR

Expression

Yeast

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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px330 Rosa26 AscI LH291Gu1 (LH500)

Plasmid

#99628

Purpose

Plasmid used to target the pre-modified Rosa26 allele with Cas9

Depositor

Rui Benedito

Publication

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Cas9

Use

CRISPR

Tags

3xFLAG

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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PiggyBac dCas9-VPR

Plasmid

#191269

Purpose

piggyBac integration and dox-inducible expression of VPR-dCas9-HA-tagBFP

Depositor

Joanna Wysocka

Publication

Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

Inserts

VPR-dCas9-HA-tagBFP
rtTA3-IRES-PURO

Use

CRISPR

Tags

HA and tagBFP

Expression

Mammalian

Available since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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evoCas9

Plasmid

#138558

Purpose

Expresses human codon-optimized evolved SpCas9 and blasticidin resistance: EFS promoter-evoCas9-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Publication

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

evoCas9

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

M495V, Y515N, K526E, R661Q

Promoter

EFS

Available since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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PiggyBac dCas9-KRAB

Plasmid

#191314

Purpose

piggyBac integration and dox-inducible expression of KRAB-dCas9-HA-tagBFP

Depositor

Joanna Wysocka

Publication

Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

Inserts

KRAB-dCas9-HA-tagBFP
rtTA3-IRES-PURO

Use

CRISPR

Tags

HA and tagBFP

Expression

Mammalian

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Cas9C-VPR

Plasmid

#80933

Purpose

Expresses Cas9C-VPR in mammalian cells.

Depositor

George Church

Publication

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9C-VPR

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pHS26_10xHis_MBP_TEV_VRER-Cas9

Plasmid

#244829

Purpose

Bacterial expression of VRER Cas9 for protein purification

Depositor

Jennifer Doudna

Publication

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

VRER Cas9

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Mutation

D1135V/G1218R/R1335E/T1337R

Promoter

T7

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHS23_10xHis_MBP_TEV_SpG-Cas9

Plasmid

#244826

Purpose

Bacterial expression of SpG Cas9 for protein purification

Depositor

Jennifer Doudna

Publication

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpG Cas9

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Mutation

D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

Promoter

T7

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHS25_10xHis_MBP_TEV_EQR-Cas9

Plasmid

#244828

Purpose

Bacterial expression of EQR Cas9 for protein purification

Depositor

Jennifer Doudna

Publication

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

EQR Cas9

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Mutation

D1135E/R1335Q/T1337R

Promoter

T7

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCas9-beta

Plasmid

#169240

Purpose

Cas9 and beta expression plasmid for performing gene knockouts via oligo recombineering

Depositor

Brian Pfleger

Publication

Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

Inserts

cas9
beta

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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