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CIRTS-Gas5

Plasmid

#213172

Purpose

CIRTS RNA targeting system for targeted knockdown in neurons. CIRTS-Calm3 and Gas5 guide RNA is expressed under human Syn1 and U6 promoter, respectively.

Depositor

Timothy Bredy

Article

Liau et al Nat Commun. 2023 Nov 22;14(1):7616. doi:

Insert

CIRTS-Calm3-U6-Gas5 gRNA

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

Syn1, U6

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-RVR-A3A-N57G

Plasmid

#193657

Purpose

Expression of base editor RVR-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

RVR-dLbCas12a-hA3A-N57G, EGFP

Expression

Mammalian

Mutation

dLbCas12a-G532R/K538V/Y542R, hAPOBEC3A-N57G

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-A3B

Plasmid

#193642

Purpose

Expression of base editor dCas12a-A3B in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-hA3B, EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-A3G

Plasmid

#193643

Purpose

Expression of base editor dCas12a-A3G in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-hA3G, EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-ACE2 receptor 19-615

Plasmid

#167012

Purpose

Gateway-compatible Entry vector encoding ACE2 receptor (aa19-615) interacting with spike (S1) RBD domain from SARS-CoV-2

Depositor

Spyros Petrakis

Article

Gkekas et al J Chem Inf Model. 2024 Nov 13. doi: 10.1

Insert

ACE2 (ACE2 Human)

Expression

Mammalian

Mutation

Amino acids 19-615, No stop codon

Available Since

March 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV_Donor/Reporter-COL4A5

Plasmid

#130280

Purpose

COL4A5 mutation-specific sgRNA under the control of U6 promoter; mCherry-sgRNA target-(out of frame)EGFP expression cassette; COL4A5 donor template

Depositor

Silvestro Conticello

Article

Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

Insert

mCherry-eGFP

Use

AAV

Promoter

CMV

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX_307-SOD1E133insTT

Plasmid

#83446

Purpose

Mammalian expression of SOD1E133insTT.

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

SOD1 (SOD1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

E133insTT (after GA)

Promoter

EF-1α

Available Since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pLEX_307-SOD1E133Δ

Plasmid

#83445

Purpose

Mammalian expression of SOD1E133Δ.

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

SOD1 (SOD1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

E133Δ

Promoter

EF-1α

Available Since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pSOD1E133K-AcGFP1

Plasmid

#83442

Purpose

Expresses SOD1E133K in mammalian cells.

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

SOD1 (SOD1 Human)

Tags

AcGFP1

Expression

Mammalian

Mutation

E133K

Promoter

CMV

Available Since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pBK- Ma PylRS WT

Plasmid

#197573

Purpose

Expresses wild-type Methanomethylophilus alvus pyrrolysine tRNA synthetase under GlnS promoter. Used for Pyl tRNA-synthetase selections.

Depositor

Ryan Mehl

Article

Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

Insert

M. alvus PylRS WT

Expression

Bacterial

Promoter

GlnS (constitutive)

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pUG-UBA1

Plasmid

#104665

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

AtUBA1 (UBA1 Mustard Weed)

Use

Synthetic Biology

Mutation

BpiI/BsaI sites removed by point-mutation

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pSB819-PKR-gib

Plasmid

#20031

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

Protein kinase R

Expression

Yeast

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pET29a-hNABP2

Plasmid

#128307

Purpose

Express hNABP2 (a.k.a hSSB1 or SOSSB1) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

Human Nucleic Acid Binding Protein 2 (hNABP2) (NABP2 Human)

Tags

His

Expression

Bacterial

Mutation

Y85C- please see depositor comments below

Promoter

T7

Available Since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs-hs-HRAS

Plasmid

#52730

Purpose

retroviral expression of human HRAS

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

HRAS (HRAS Human)

Use

Retroviral

Expression

Mammalian

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only
pUC19_CoV-2 frD_ΔrE/GFP

Plasmid

#253120

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of envelope (residual AA). GFP is inserted in the locus of envelope

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

Fragment D2 SARS-CoV-2 ΔE-GFP

Expression

Bacterial

Available Since

June 9, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD ΔS/VSVG ΔE67 3*

Plasmid

#253173

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of spike, envelope, orf6, orf7a;stop codon at AA213 in orf3a; VSV glycoprotein replaces spike

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD with VSV-G

Expression

Bacterial

Available Since

June 9, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_ frD_Δ3E/VSVG Δ67a

Plasmid

#253172

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of envelope, orf6, orf7a;stop codon at AA213 in orf3a; VSV glycoprotein replaces orf3a and envelope

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD with VSV-G

Expression

Bacterial

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_ frD_Δ3E/N1 PR8 Δ67a

Plasmid

#253169

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of orf3a, envelope, orf6, orf7a; Influenza N1 (PR8) replaces orf3a and envelope

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD with Influenza N1 PR8

Expression

Bacterial

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_ frD_Δ3E/H1 PR8 Δ67a

Plasmid

#253168

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of orf3a, envelope, orf6, orf7a; Influenza H1 (PR8) replaces orf3a and envelope

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD with Influenza H1 PR8

Expression

Bacterial

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD2 ΔE7 3* Δ6/H1 PR8

Plasmid

#253147

Purpose

For recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), with deletion of envelope, orf6, orf7a;stop codon at AA213 in orf3a; Influenza H1 (PR8) replaces orf6

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD2 with Influenza H1 PR8

Expression

Bacterial

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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