163,991 results

1. AAV-pCbh-SpRY[C-term]-gRNAentry (CA20)

   Plasmid

   #197511

   Purpose

   Cbh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpRY and BsmBI entry cassette to clone SpCas9 gRNA spacer

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   AAV-[ITR]-pCbh-BPNLS-NpuC-SpRY[C-term]-BPNLS-gRNA[BsmBI]-pU6-[ITR]
   

   Use

   AAV and CRISPR

   Tags

   BPNLS and BPNLS-NpuC(intein)

   Expression

   Mutation

   C-terminal mutations of SpRY(L1111R/D1135L/S1136W…

   Promoter

   Cbh

   Available since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pg-HIF-1alpha-EGFP

   Plasmid

   #87204

   Purpose

   C-terminally EGFP-tagged human HIF-1a

   Depositor

   Violaine Sée

   Article

   Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64.

   Insert

   HIF-1a (HIF1A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   L40F, M88V, Q814R

   Promoter

   CMV

   Available since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC1-CMV-DAAO-NES

   Plasmid

   #141107

   Purpose

   Expresses DAAO with nuclear export signal in mammalian cells

   Depositor

   Vsevolod Belousov

   Article

   Bogdanova et al Curr Protoc Chem Biol. 2017 Jun 19;9(2):

   Insert

   DAAO-NES
   

   Use

   Tags

   Nuclear export signal

   Expression

   Mammalian

   Mutation

   deleted DAAO amino acids 367-368

   Promoter

   CMV

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-DN-hCUL4B-FLAG

   Plasmid

   #15822

   Depositor

   Wade Harper

   Article

   Jin et al Methods Enzymol. 2005;399:287-309. doi:

   Insert

   Cullin4B (CUL4B Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Dominant negative: N594

   Promoter

   Available since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST (AAV9)

   Viral Prep

   #174007-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST (#174007). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST plasmid DNA. Syn-driven, Cre-dependent expression of calcium sensor GCaMP8s and soma-targeted ChrimsonR. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Mark Histed

   Article

   LaFosse et al eNeuro. 2023 Mar 29;10(3):ENEURO.0378-22

   Promoter

   Syn

   Tags

   None

   Available since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3-DN-hCUL5-FLAG

   Plasmid

   #15823

   Depositor

   Wade Harper

   Article

   Jin et al Methods Enzymol. 2005;399:287-309. doi:

   Insert

   Cullin5 (CUL5 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Dominant negative: N441

   Promoter

   Available since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-CMV-Nrf2

   Plasmid

   #67636

   Purpose

   AAV vector expressing Nrf2 gene

   Depositor

   Connie Cepko

   Article

   Xiong et al J Clin Invest. 2015 Apr;125(4):1433-45.

   Insert

   Nrf2 (Nfe2l2 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-hMC4R

   Plasmid

   #179315

   Purpose

   Express human melanocortin 4 receptor (GFP tagged) in mammalian cells

   Depositor

   Christian Gruber

   Article

   Muratspahic et al ACS Pharmacol Transl Sci. 2023 Sep 26;6(

   Insert

   melanocortin 4 receptor (MC4R Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKS1

   Plasmid

   #84079

   Purpose

   Expresses the non-toxic fragment C of tetanus toxin

   Depositor

   Neil Fairweather

   Article

   Sinha et al Mol Microbiol. 2000 Sep;37(5):1041-51.

   Insert

   Tetanus neurotoxin fragment C
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSL1521 (pSPIN, pSC101* backbone)

    Plasmid

    #160729

    Purpose

    Single-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins, crRNA, and donor DNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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