We narrowed to 28,468 results for: CAL

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pCLC3sFlag

Plasmid

#52422

Purpose

mammalian expression of flag tagged rat CLC3 short transcript

Depositor

Steven Weinman

Article

Shimada et al Am J Physiol Gastrointest Liver Physiol.

Insert

CLC3 (Clcn3 Rat)

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

May 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGL5

Plasmid

#48745

Purpose

Expression of luciferase driven by truncated mPer2 promoter region (bases -1128 to +2064 with respect to transcription start site at +1)

Depositor

Joseph Takahashi

Article

Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

Insert

truncated mPer2 promoter/enhancer region (-1128 to +2064)

Use

Luciferase

Mutation

truncated promoter region (see pGL6)

Available Since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only
pT7-AsCas12a_crRNA-site3 (RTW549)

Plasmid

#160138

Purpose

T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #3

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Insert

AsCas12a crRNA with spacer #3 (spacer=CTGATGGTCCATGTCTGTTACTC)

Use

In vitro transcription of sgrna from t7 promoter

Promoter

T7

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA6/ERGIC2-WT

Plasmid

#51479

Purpose

Expresses ERGIC2-WT in mammalian cells

Depositor

Simon Kwok

Article

Kwok et al DNA Cell Biol. 2001 Jun;20(6):349-57.

Insert

ERGIC2-WT (ERGIC2 Human)

Tags

none

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

March 6, 2014

Availability

Academic Institutions and Nonprofits only
pFX-GalT-Venus

Plasmid

#174472

Purpose

Express Venus with golgi apparatus-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Venus with golgi apparatus-targeting sequence

Tags

β1,4-galactose transferase

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-Venus-KRasCT

Plasmid

#175834

Purpose

Express Venus with plasma membrane-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Venus with plasma membrane-targeting sequence

Tags

C-terminal hypervariable region of K-Ras 4B

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-Tom20-Venus

Plasmid

#174187

Purpose

Express Venus with mitochondria-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Venus with mitchondria-targeting sequence

Tags

the mitochondria-targeting sequence of TOM20

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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Human T (BRACHYURY) sgRNA

Plasmid

#59726

Purpose

Plasmid encoding sgRNA to generate T (BRACHYURY) knock-out mutant human cells

Depositor

Jacob Hanna

Article

Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

Insert

hT sgRNA (TBXT Human)

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 29, 2014

Availability

Academic Institutions and Nonprofits only
EGFP-bSV1_1-1009

Plasmid

#129471

Purpose

Mammalian expression of the N-terminus + central domain of bovine SV1

Depositor

Elizabeth Luna

Article

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

Supervillin (SVIL Bovine)

Expression

Mammalian

Mutation

contains amino acids 1-1009

Promoter

CMV

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pHH0103_NCK1-1/3

Plasmid

#91472

Purpose

Protein expression and purification of human SH3 domain construct NCK1-1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NCK1-1/3 (NCK1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-SAC1-HLx8

Plasmid

#108139

Purpose

SAC1 with extended linker

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:SACM1L(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)

Expression

Mammalian

Mutation

SACM1L(1-520):[EAAAR]8:SACM1L(521-587)

Promoter

CMV

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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CLDN8_pCSdest

Plasmid

#53811

Purpose

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

CLDN8 (CLDN8 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
JPF0522

Plasmid

#124013

Purpose

Encodes crippled pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB_pEF1ac-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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p21 promoter F

Plasmid

#16467

Depositor

Bert Vogelstein

Article

el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

Insert

p21 promoter (CDKN1A Human)

Expression

Mammalian

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pPKm-243

Plasmid

#90506

Purpose

mOrange and mitochondrial-tagged sfGFP (pSIN-OFP-mitosfGFP)

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

mOrange

Use

Lentiviral

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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JPF0454

Plasmid

#124039

Purpose

Encodes pPGK1 driving expression of spCas9 P2A mAzamiGreen in a 2nd gen. Lenti Virus destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pHR_pPGK1-spCAS9-P2A-mAzamiGreen

Expression

Mammalian

Available Since

Feb. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3 humanN-Flag SadBS

Plasmid

#66881

Purpose

Mammalian expression vector for expressing N-terminally Flag-tagged short isoform of SadB

Depositor

Maria Alvarado-Kristensson

Article

Eklund et al J Biol Chem. 2014 Aug 1;289(31):21360-73

Insert

SadB (BRSK1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only
pGEL065

Plasmid

#124896

Purpose

For genome editing in plant by SpCas9 variant(SpCas9-NGv1)

Depositor

Yong Zhang

Article

Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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KHBD00486

Plasmid

#39591

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG5965 (woc Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only
pCAG-ChroME(F243Y)-mRuby2-ST

Plasmid

#109127

Purpose

Modified cation channelrhodopsin ChroME fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

ChroME(F243Y)-mRuby2-ST

Expression

Mammalian

Promoter

CAG

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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