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pD649-HAsp-SEMA3F-Fc(DAPA)-AviTag-6xHis

Plasmid

#156977

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SEMA3F (SEMA3F Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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Flag‐mTim3 (del.Cyto)

Plasmid

#49209

Purpose

expresses Flag tagged mouse Tim3 fragment (no cytosolic domain)

Depositor

Lawrence Kane

Article

Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

Insert

Tim3 (del.Cyto) (Havcr2 Mouse)

Tags

CD8 signal sequence and FLAG

Expression

Mammalian

Mutation

cytosolic domain deleted, no Tim3 signal sequence…

Promoter

Ef1a

Available Since

Dec. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pHR RPS27A K113R-3xFlag_IRES-GFP

Plasmid

#198391

Purpose

RPS27A K113R (ubiquitin/ribosomal protein eS31 fusion) expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

RPS27A (RPS27A Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

ubiquitination site mutant (K113R)

Promoter

EF1A

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-Venus-cpVenus-FLARE-AKAR

Plasmid

#123337

Purpose

Plasma membrane-targeted yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Lyn-Venus-cpVenus-FLARE-AKAR

Tags

N-terminal targeting sequence from Lyn kinase, Ve…

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Venus-cpVenus-FLARE-CKAR

Plasmid

#123343

Purpose

Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Venus-cpVenus-FLARE-CKAR

Tags

6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4 3xFLAG Halo delta 122-130 nsp1

Plasmid

#175425

Purpose

Expresses delta 122-130 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV2 delta 122-130 nsp1

Tags

3XFLAG Halo

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-TNFRSF6B-COMP5AP-AviTag-9xHis

Plasmid

#157277

Purpose

Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

TNFRSF6B (TNFRSF6B Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pRC/CMV-TYK2K930R-FF-VSV

Plasmid

#139348

Purpose

Expression of kinase-dead TYK2-K930R missing the two tyrosines at the activation loop

Depositor

Sandra Pellegrini

Article

Gauzzi et al J Biol Chem. 1996 Aug 23;271(34):20494-5

Insert

TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)

Tags

VSV

Expression

Mammalian

Mutation

V362F-K930R-Y1054F-Y1055F

Promoter

CMV

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pRC/CMV-TYK21054-5FF-VSV

Plasmid

#139347

Purpose

Expression of TYK2 protein mutated in the two tyrosines (Y1054F/Y1055F) of the activation loop

Depositor

Sandra Pellegrini

Article

Gauzzi et al J Biol Chem. 1996 Aug 23;271(34):20494-5

Insert

TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)

Expression

Mammalian

Mutation

V362F-Y1054F-Y1055F

Promoter

CMV

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pMP108 Nanog-Venus-2a-mCherry

Plasmid

#159743

Purpose

Nanog targeting vector to insert fluorescent reporter of protein and gene expression levels from endogenous locus in mouse embryonic stem cells. Please see depositor comments for more detail.

Depositor

Sergi Regot

Article

Pokrass et al Dev Cell. 2020 Nov 9;55(3):328-340.e5. d

Insert

Nanog homeobox (Nanog Mouse)

Use

Mouse Targeting

Tags

Venus-2a-mCherry

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-LAIR2-Fc(DAPA)-AviTag-6xHis

Plasmid

#156520

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

LAIR2 (LAIR2 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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-161+80pCalb2/E2F2/NRF-1(-64)-like

Plasmid

#66742

Purpose

luciferase reporter for Calb2 promoter (-161to +80, mutant)

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

Insert

Calb2 promoter (-161bp+80) (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

CGCG(-64) to TACA

Promoter

Calb2

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pLJD-KLF4-ER

Plasmid

#34596

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Use

Retroviral

Tags

Myc epitope tag and estrogen receptor fusion

Expression

Mammalian

Promoter

LTR

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only
Flag‐mTim3 (T2 truncation)

Plasmid

#49211

Purpose

expresses Flag tagged mouse Tim3 (T2 truncation)

Depositor

Lawrence Kane

Article

Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

Insert

Tim3 T2 truncation (Havcr2 Mouse)

Tags

FLAG and signal sequence

Expression

Mammalian

Mutation

T2 truncation ( lacks all but one tyrosine, which…

Promoter

EF1a

Available Since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pOPINK-ALG13 (OTU, aa 216-353)

Plasmid

#61414

Purpose

Expresses human ALG13 (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

ALG13 (Putative bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13) (ALG13 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-215 and aa 354-1137.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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Flag‐mTim3 (T1 truncation)

Plasmid

#49210

Purpose

expresses Flag tagged mouse Tim3 (T1 truncation)

Depositor

Lawrence Kane

Article

Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

Insert

Tim3 T1 truncation (Havcr2 Mouse)

Tags

FLAG and signal sequence

Expression

Mammalian

Mutation

T1 truncation ( contains all but the three most C…

Promoter

EF1a

Available Since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only

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161+80pCalb2/mutCREB(-2)-like

Plasmid

#66751

Purpose

luciferase reporter for Calb2 promoter (-161to +80, mutant)

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

Insert

Calb2 promoter (-161bp +80) (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

GCGC (-2) to TAGA

Promoter

Calb2

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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-161+80pCalb2/mutNRF-1(-41)-like

Plasmid

#66744

Purpose

luciferase reporter for Calb2 promoter (-161to +80, mutant)

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

Insert

Calb2 promoter (-161bp+80) (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

CGC (-41) to ATT

Promoter

Calb2

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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-161+80pCalb2/dmutNRF-1(-41;-35)-like

Plasmid

#66741

Purpose

luciferase reporter for Calb2 promoter (-161to +80, mutant)

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

Insert

Calb2 promoter (-161bp+80) (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

CGCAGGCGC (-41;-35) to ATTAGGATT

Promoter

Calb2

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE-WDR12-S127F

Plasmid

#116707

Purpose

Lentiviral expression of WDR12 S127F

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

WDR12 (WDR12 Human)

Use

Lentiviral

Mutation

S127F

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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