We narrowed to 23,538 results for: Sis

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GST_LIM234

Plasmid

#182039

Purpose

For expression of human paxillin LIM domains 2-4 with N-terminal GST fusion protein in E. coli.

Depositor

Vesa Hytönen

Article

Ripamonti et al Commun Biol. 2021 Mar 29;4(1):380. doi:

Insert

LIM234 (PXN Human)

Tags

GST

Expression

Bacterial

Mutation

LIM domains 2-4 only

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO/MACROD1

Plasmid

#172578

Purpose

For generation of FlpIn lines with untagged MACROD1

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD1 (MACROD1 Human)

Tags

none

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MCS-BirA(R118G)-HA-TARG1

Plasmid

#172583

Purpose

For transient expression of C-terminal BirA-tagged TARG1

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

TARG1 (OARD1 Human)

Tags

BirA(R118G)-HA

Expression

Mammalian

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNanos3-del4-20_X

Plasmid

#147945

Purpose

Mammalian Expression of HsNanos3-del4-20

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos3-del4-20 (NANOS3 Human)

Expression

Mammalian

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-Ub-VPS13D

Plasmid

#176462

Purpose

expressing GFP-Ub marked VPS13D in mammalian cells. GFP-Ub will be cleaved by DUBs to express untagged VPS13D

Depositor

Richard Youle

Article

Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

VPS13D (VPS13D Human)

Tags

GFP-Ub

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-Ub-VPS13D N3521S

Plasmid

#176463

Purpose

expressing VPS13D patient mutant N3521S in mammalian cells

Depositor

Richard Youle

Article

Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

VPS13D (VPS13D Human)

Tags

GFP-Ub

Expression

Mammalian

Mutation

N3521S mutation

Promoter

CMV

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-14

Plasmid

#160714

Purpose

For C-terminal tags with Trichoderma optimised Synthetic 3X-c-Myc tag peptide (EQKLISEEDL) bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

Trichoderma optimised Synthetic 3X-c-Myc tag peptide (EQKLISEEDL)

Use

Synthetic Biology

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmMe31B-mut1_E

Plasmid

#146203

Purpose

Insect Expression of DmMe31B-mut1

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

DmMe31B-mut1 (me31B Fly)

Expression

Insect

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCASCADE-xylA

Plasmid

#158611

Purpose

Low phosphate inducible gRNA silencing the xylA promoter

Depositor

Michael Lynch

Article

Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

Insert

xylA guide array

Expression

Bacterial

Promoter

E . coli ugpB gene promoter, low phosphate induct…

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCASCADE-udhA-xylA

Plasmid

#158612

Purpose

Low phosphate inducible gRNA array to silence the xylA and udhA promoters

Depositor

Michael Lynch

Article

Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

Insert

udhA-xylA guide array

Expression

Bacterial

Promoter

E . coli ugpB gene promoter, low phosphate induct…

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCASCADE-gltA2-xylA

Plasmid

#158613

Purpose

Low phosphate inducible gRNA array to silence the xylA and gltA2 promoters

Depositor

Michael Lynch

Article

Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

Insert

gltA2-xylA guide array

Expression

Bacterial

Promoter

E . coli ugpB gene promoter, low phosphate induct…

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCASCADE-zwf-xylA

Plasmid

#158614

Purpose

Low phosphate inducible gRNA array to silence the xylA and zwf promoters

Depositor

Michael Lynch

Article

Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

Insert

zwf-xylA guide array

Expression

Bacterial

Promoter

E . coli ugpB gene promoter, low phosphate induct…

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-mSpata33-PA

Plasmid

#179129

Purpose

Expression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal.

Depositor

Masahito Ikawa

Article

Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

Insert

spermatogenesis associated 33 (Spata33 Mouse)

Tags

PA

Expression

Mammalian

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pET-epELM-5

Plasmid

#179391

Purpose

Express a peptide (reside from 550 through 600) of human ELMSAN1 (Q6PJG2) in bacteria

Depositor

Shiro Iuchi

Article

Iuchi et al Commun Biol. 2021 Dec 15;4(1):1399. doi:

Insert

ELMSAN1 (MIDEAS Human)

Tags

6x His

Expression

Bacterial

Promoter

T7 lac

Available Since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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Halo-WIPI2B K88E

Plasmid

#175028

Purpose

Expresses WIPI2B (K88E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1

Depositor

Andrea Stavoe

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2B (K88E) (WIPI2 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

Lysine 88 to Glutamate (AAG to GAG)

Promoter

CMV

Available Since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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Halo-WIPI2B I92E

Plasmid

#175029

Purpose

Expresses WIPI2B (I92E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1

Depositor

Andrea Stavoe

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2B (I92E) (WIPI2 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

Isoleucine 92 to Glutamate (ATC to GAA)

Promoter

CMV

Available Since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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Halo-WIPI2B C93E

Plasmid

#175030

Purpose

Expresses WIPI2B (C93E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1

Depositor

Andrea Stavoe

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2B (C93E) (WIPI2 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

Cysteine 93 to Glutamate (TGC to GAG)

Promoter

CMV

Available Since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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Halo-WIPI2B K128E

Plasmid

#175031

Purpose

Expresses WIPI2B (K128E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1

Depositor

Andrea Stavoe

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2B (K128E) (WIPI2 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

Lysine 128 to Glutamate (AAG to GAG)

Promoter

CMV

Available Since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCm N-term MBP SMT3 NikJC199U

Plasmid

#174363

Purpose

Expression plasmid with a ULP1 removable N-term MBP SUMO tag, a TEV removable C-term 8xHis tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

Depositor

Brandon Greene

Article

Caceres et al Methods Enzymol. 2022;662:119-141. doi:

Insert

nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]

Tags

8xHis, MBP, SMT3, and TEV

Expression

Bacterial

Mutation

C199U

Promoter

T7

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD2KN0899

Plasmid

#177092

Purpose

MoClo Level 2 plasmid containing multiple transcriptional units for transient expression of CrSTR, CrSLS, CrLAMT, CrTDC, and Cr7-DLH driven by 4x opaatB

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

CrSTR, CrSLS, CrLAMT, CrTDC, and Cr7-DLH

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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